口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (3): 258-261.DOI: 10.13701/j.cnki.kqyxyj.2017.03.006

• 基础研究论著 • 上一篇    下一篇

大豆苷元对骨质疏松模型大鼠拔牙后牙槽窝骨修复的影响

张雨1，林赳1#，高巍1，胡懿1，高一1，熊文碧2，潘剑1*   

  1. 1. 四川大学口腔疾病研究国家重点实验室,华西口腔医院牙槽外科 四川 成都 610041;
    2. 四川大学基础与法医学系 四川 成都 610041
  • 收稿日期:2016-09-02 出版日期:2017-03-20 发布日期:2017-03-22
  • 通讯作者: 潘剑,E-mail:jianpancn@163.com
  • 作者简介:张雨(19~ ),女,四川成都人,学士,主要从事牙槽外和种植骨生成研究。林赳(19~ ),男,湖南邵阳人,学士,主要从事牙槽外、种植骨生成和牙周组织再生研究。
  • 基金资助:
    成都市科技局人口与健康计划基金资助项目(编号:12PPYB044SF-002)四川大学创新创业训练计划资助项目(编号:20140310)

Effects of Daidzein on Bone Rebuiling of Alveolar Fossa after Tooth Extraction in Osteoporotic-model Rats.

ZHANG Yu1, LIN JIU1#, GAO Wei1, HU Yi1, GAO Yi1, XIONG Wen-bi2, PAN Jian1*.   

  1. 1. State Key Laboratory of Oral Diseases, Department of Alveolar Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China;
    2. West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China
  • Received:2016-09-02 Online:2017-03-20 Published:2017-03-22

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摘要： 目的:研究大豆苷元(Daidzein, Da)对骨质疏松模型大鼠拔牙后牙槽窝处骨修复的影响。方法:24只3月龄大鼠随机均分为4组:假手术组(Sham)、雌激素组(EG)、大豆苷元组(Da)(75 mg/kg·d-1)和去卵巢组(Ovx)。摘除卵巢1周后,所有大鼠拔去双侧上颌第一磨牙,第12周检测血清钙、磷含量及总碱性磷酸酶(alkaline phosphatase, ALP)活性,制作左侧上颌骨拔牙窝处骨组织切片,于光镜下观察牙槽窝骨修复情况。结果:去卵巢后大鼠血清钙含量(2.10±0.04)及ALP活性(3.47±0.36)显著性降低(P<0.01),大豆苷元能显著提高去卵巢后大鼠血清钙含量(2.27±0.05)及ALP活性(4.49±0.38)(P<0.01)。骨形态观察表明大豆苷元对于牙槽窝的骨保存功能与雌激素相似,与Sham组骨形态结构较接近。结论:大豆苷元能有效改善骨质疏松模型大鼠拔牙后血清钙含量和ALP水平,减少牙槽窝骨吸收并恢复骨形态。

关键词: 大豆苷元, 骨质疏松, 微量元素, 拔牙, 骨修复

Abstract: Objective: To explore the effect of Daidzein (Da) on the alveolar bone rebuilding after tooth extraction in osteoporotic-model rats. Methods: Twenty-four 3-months-old female SD rats were randomly divided into four groups: sham group (Sham), estrogen group (EG), ovariectomized group (Ovx), ovariectomized and Daidzein group (Da). Bilateral maxillary first molars of all rats were extracted at the 7th day post ovarian surgery. The values of serum calcium, phosphorus and alkaline phosphatase (ALP) were recorded at the 12th week. Meanwhile, bone tissue sections of the left side of alveolar bone were made to observe bone rebuilding. Results: The value of serum calcium(2.49±0.02) and ALP(5.31±0.5) of ovariectomized rats decreased significantly(P<0.01), Daidzein could significantly improve the value of calcium(2.27±0.05)and the activity of ALP(4.49±0.38) in serum of ovariectomized rats(P< 0.01).Morphological observation in alveolar bone tissue around the tooth fossa showed that the function of bone preservation of Daidzein was similar to estrogen, and was relatively similar to that of Sham group. Conclusion: Daidzein can remarkably improve the value of calcium and ALP in serum, reduce resorption of alveolar bone around the tooth fossa, and rebuild the bone morphology after extraction in osteoporotic-model rats.

Key words: Daidzein , Osteoporosis , Trace elements, Tooth extraction, Bone rebuilding

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