口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (3): 228-233.DOI: 10.13701/j.cnki.kqyxyj.2016.03.005

• 基础研究论著 • 上一篇    下一篇

负载CGRP及SP的明胶缓释微球用于修复家兔骨质疏松模型骨缺损的研究

刘伟, 陈杰, 胡开进, 张林林, 丁宇翔*   

  1. 第四军医大学军事口腔医学国家重点实验室口腔外科 陕西 西安 710032
  • 收稿日期:2015-08-13 出版日期:2016-03-28 发布日期:2016-03-29
  • 通讯作者: 丁宇翔,E-mail:yxding@fmmu.edu.cn
  • 作者简介:刘伟(1988~ ),男,山东人,博士,主治医师,主要从事口腔颌面外科临床研究工作。
  • 基金资助:
    国家自然科学基金项目(编号:81271103)

Sustain-released Gelatin Microspheres Loaded with CGRP/SP for Repairing Bone Defect of Osteoporosis Rabbits.

LIU Wei, CHEN Jie, HU Kai-jin, ZHANG Lin-lin, DING Yu-xiang*   

  1. State Key Laboratory of Military Stomatology, Department of Oral Surgery,School of Stomatology, The Fourth Military Medical University,Xi'an 710032, China
  • Received:2015-08-13 Online:2016-03-28 Published:2016-03-29

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摘要： 目的: 将负载不同浓度的降钙素基因相关肽(CGRP)及P物质(SP)的明胶缓释微球植入骨质疏松兔的股骨缺损中,观察其对骨缺损修复的影响。方法: 通过去势方法建立兔骨质松模型,利用乳化交联的方法制备负载不同浓度的CGRP及SP的明胶缓释微球。将24只骨质疏松兔随机分组,于股骨髁部制备骨缺损并分别植入负载10-6 mol/L、10-8 mol/L和10-10 mol/L的SP以及CGRP的明胶微球,3个月后处死观察结果。结果: 负载不同浓度的CGRP及SP均能有效地促进成骨,影像学结果示SP在10-6 mol/L时其骨体积分数最高,并且能有效提高骨小梁数量,降低骨小梁间的间距(P<0.05),CGRP在浓度为和10-8 mol/L时效果即达到上述效果;而组织学结果示SP和CGRP均在浓度为10-6 mol/L时其新生骨组织比例最高(P<0.05)。结论: 负载不同浓度的CGRP及SP均能有效地促进骨质疏松兔骨缺损的成骨,在所研究的浓度中,高浓度的效果优于低浓度的效果。

关键词: 降钙素基因相关肽, P物质, 明胶微球, 骨质疏松, 骨缺损

Abstract: Objective: To evaluate the impact of sustain-released gelatin microspheres loaded with CGRP/SP on the osteogenesis of the bone defect in osteoporosis rabbits. Methods: New Zealand white rabbit model was established by ovariectomy. The DEXA was used to detect the rabbits lumbar vertebrae BMD. Sustain-released gelatin microspheres loaded with 10-6mol/L, 10-8mol/L and 10-10mol/L of CGRP/SP were prepared via emulsion cross-linking method. Twenty-four rabbit models were randomly assigned to receive sustain-released gelatin microspheres loaded with different concentrations of CGRP/SP into the bone defects. The release in vitro within 1 month was measured by ELISA method. The rabbits were killed 3 months after the implanting to evaluate the impact of osteogenesis. Results: Gelatin microspheres loaded with CGRP/SP had better effect than the blank control group. The effect reaches the best when the concentration of SP was 10-6mol/L and CGRP was 10-8mol/L. And the effect reached the best when the concentration of SP and CGRP was 10-6mol/L. Conclusion: Gelatin microspheres loaded with CGRP/SP could improve the new bone formation significantly in contrast to the blank control group. Higher concentration was better.

Key words: Calcitonin gene related peptide , Substance P , Gelatin microspheres, Bone osteoporosis , Bone defect

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