浓缩生长因子促进骨再生的实验研究

doi:10.13701/j.cnki.kqyxyj.2017.09.011

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (9): 954-957.DOI: 10.13701/j.cnki.kqyxyj.2017.09.011

浓缩生长因子促进骨再生的实验研究

罗婷苑，张森林^*

南方医科大学南京临床医学院,南京军区南京总医院口腔科江苏南京 210002

收稿日期:2017-03-01 出版日期:2017-09-20 发布日期:2017-09-27
通讯作者: 张森林,E-mail: doczhangsl@126.com
作者简介:罗婷苑(1990~ ),女,广东惠州人,硕士,主要从事口腔颌面外科临床治疗工作。
基金资助:
南京军区医药卫生科研基金课题(编号:11MA091)南京军区医学科技创新课题(编号:15DX020)

Research on Concentrated Growth Factor in Bone Regeneration

LUO Ting-yuan, ZHANG Sen-lin^*

Department of Stomatology, Jinling Hospital, Nanjing School of Clinical Medicine, Southern Medical University, Nanjing 210002, China.

Received:2017-03-01 Online:2017-09-20 Published:2017-09-27

摘要/Abstract

摘要： 目的:评价珊瑚羟基磷灰石(CHA)联合浓缩生长因子(CGF)修复骨缺损的效果。方法:选24只新西兰兔,随机分为3组,每组8只,在每只兔的两侧颅骨上各制备1个直径10 mm圆形缺损。第1组动物两侧颅骨缺损分别植入CHA/CGF和CGF,第2组分别植入CHA/CGF和空白对照,第3组分别植入CGF和空白对照。术后6周和12周取材,通过Micro-CT及组织学观察评价骨修复效果。结果:Micro-CT观察显示,术后6周和12周,CHA/CGF组新骨形成量和骨密度最高,CGF次之,空白对照组最小(P<0.05)。组织学观察显示,术后6周,在CHA/CGF组整个缺损区的CHA孔隙内有散在的新骨形成,CGF组在邻近骨床的缺损区新骨长入;术后12周,CHA/CGF组的CHA孔隙内有大量新骨形成并相互融合,部分CHA降解吸收,而CGF组的缺损区新生骨进一步向缺损中央长入,但缺损中央区新生骨质较薄或仅有结缔组织充填。结论:将CHA与CGF联合使用能有效地促进骨愈合。

关键词: 珊瑚羟基磷灰石, 浓缩生长因子, 骨缺损, 修复

Abstract: Objective: To evaluate local bone formation following surgical implantation of coralline hydroxyapatite (CHA) in combination with concentrated growth factor (CGF) using a rabbit calvarial defect model. Methods: Twenty-four rabbits were included in this randomized study. A circular defect in a diameter of 10mm was prepared on the parietal bone. The first group was filled with CHA/CGF and CHA. The second group was filled with CHA/CGF and void. The third group was filled with CGF and void. The bone formation was analyzed with microscopic computed tomography (micro-CT) and histological examination after 6 and 12 weeks. Results: With micro-CT analysis, bone volume and bone mineral density in the CHA/CGF group were the highest, followed by CGF group, and the blank control group was the lowest (P<0.05). Histological examination showed that new bone was distributed throughout the CHA scaffold in the specimens of CGF/CHA group after 6 weeks, and new bone appeared in the periphery region of the defects in the CGF group. The bone defects of CGF/CHA group were repaired fully with bone after 12 weeks, while those of the CGF group were repaired partly with bone. Conclusion: Combination of CHA and CGF could enhance the bone healing considerably.

Key words: Coralline hydroxyapatite, Concentrated growth factors, Bone defect, Repairment

中图分类号:

R782.1

罗婷苑，张森林. 浓缩生长因子促进骨再生的实验研究[J]. 口腔医学研究, 2017, 33(9): 954-957.

LUO Ting-yuan, ZHANG Sen-lin. Research on Concentrated Growth Factor in Bone Regeneration[J]. Journal of Oral Science Research, 2017, 33(9): 954-957.

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浓缩生长因子促进骨再生的实验研究

Research on Concentrated Growth Factor in Bone Regeneration

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