RAGE阻断剂FPS-ZM1对高糖环境人牙周膜成纤维细胞增殖、迁移的影响

doi:10.13701/j.cnki.kqyxyj.2017.12.005

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (12): 1258-1261.DOI: 10.13701/j.cnki.kqyxyj.2017.12.005

RAGE阻断剂FPS-ZM1对高糖环境人牙周膜成纤维细胞增殖、迁移的影响

郭玲,张云水,詹聃婷,李汪阳,丁农乐^*

西南医科大学附属医院口腔修复科四川泸州 646000

收稿日期:2017-07-05 出版日期:2017-12-20 发布日期:2018-01-03
通讯作者: 丁农乐,E-mail:372083745@qq.com
作者简介:郭玲(1977~ ),女,重庆人,博士,副教授,主要从事口腔种植修复临床、教学和科研工作。
基金资助:
四川省2017年省级科技计划应用基础研究项目(编号:2017JY0111)；四川省卫生和计划生育委员会课题(编号:16PJ174)

Effects of RAGE Inhibitor FPS-ZM1 on hPDLFs Proliferation and Migration in High Glucose.

GUO Ling, ZHANG Yuan-shui, ZHAN Dan-ting, LI Wang-yang, DING Nong-le^*.

Department of Prosthodontics, Orofacial Reconstruction and Regeneration Laboratory of Southwest Medical University, School/Hospital of Stomatology, Southwest Medical University, Luzhou 646000, China.

Received:2017-07-05 Online:2017-12-20 Published:2018-01-03

摘要/Abstract

摘要： 目的: 通过FPS-ZM1封闭RAGE功能,观察拮抗RAGE对于高糖环境下hPDLFs增殖、迁移的影响,探讨将RAGE作为药物靶点改善高糖环境下牙周膜损伤修复的可行性。方法: 采用Western Blot检测高糖环境中hPDLFs细胞RAGE的表达,利用不同浓度FPS-ZM1体外培养hPDLFs细胞,分别通过CCK-8实验和划痕实验测定细胞的增殖能力和迁移能力。结果: 高糖环境下hPDLFs的RAGE表达明显升高。FPS-ZM1对hPDLFs的增值有明显的促进作用,以250nM时促增值效果最好,且FPS-ZM1组细胞迁移能力大于无FPS-ZM1组。结论: 在一定浓度范围内,FPS-ZM1能促进高糖环境hPDLFs的增殖和迁移。

关键词: hPDLFs, FPS-ZM1, 增殖, 迁移

Abstract: Objective: To investigate the RAGE inhibition by FPS-ZM1 on proliferation and migration of human periodontal ligament fibroblasts (hPDLFs) in high glucose environment. Methods: Western-blot was used to detect the expression of RAGE in hPDLFs in high glucose environment. Different concentrations of FPS-ZM1 were added to cultivate hPDLFs cells in vitro, CCK-8 assay was used to determine the cell proliferation, and the scratch experiment was used to detect the migration ability of cells. Results: The expression of RAGE protein in hPDLFs cells cultured in high glucose group was significantly higher than that in low glucose group. FPS-ZM1 promoted the proliferation and migration ability of hPDLFs, and the optimal concentration is 250 nmol/L. Conclusion: FPS-ZM1 can promote the proliferation and migration abilities of hPDLFs in vitro.

Key words: hPDLFS, FPS-ZM1 , Proliferation, Migration

中图分类号:

R781.4

郭玲,张云水,詹聃婷,李汪阳,丁农乐. RAGE阻断剂FPS-ZM1对高糖环境人牙周膜成纤维细胞增殖、迁移的影响[J]. 口腔医学研究, 2017, 33(12): 1258-1261.

GUO Ling, ZHANG Yuan-shui, ZHAN Dan-ting, LI Wang-yang, DING Nong-le.. Effects of RAGE Inhibitor FPS-ZM1 on hPDLFs Proliferation and Migration in High Glucose.[J]. Journal of Oral Science Research, 2017, 33(12): 1258-1261.

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RAGE阻断剂FPS-ZM1对高糖环境人牙周膜成纤维细胞增殖、迁移的影响

Effects of RAGE Inhibitor FPS-ZM1 on hPDLFs Proliferation and Migration in High Glucose.

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