口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (12): 1274-1277.DOI: 10.13701/j.cnki.kqyxyj.2017.12.009

• 基础研究论著 • 上一篇    下一篇

不同材料在碳酸饮料所致脱矿釉质再矿化中的作用

梁向阳,孔晶晶,李春年*,李安   

  1. 河北医科大学口腔医院,河北省口腔医学重点实验室 河北 石家庄 050017
  • 收稿日期:2017-05-05 出版日期:2017-12-20 发布日期:2018-01-03
  • 通讯作者: 李春年,E-mail:lchunnian@163.com
  • 作者简介:梁向阳(1990~ ),女,硕士,医师,主要从事口腔内科学研究工作。
  • 基金资助:
    河北省中医药类科研计划课题(编号:2017177)；河北医科大学大学生创新性实验计划项目(编号:USIP2016158)

Effects of Different Materials on Remineralization of Demineralized Enamel Caused by Carbonated Beverage.

LIANG Xiang-yang, KONG Jing-jing, LI Chun-nian*, LI An.   

  1. Hospital of Stomatology, Hebei Medical University, The Key Laboratory of Stomatology, Shijiazhuang 050017, China.
  • Received:2017-05-05 Online:2017-12-20 Published:2018-01-03

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摘要： 目的: 观察碳酸饮料造成的实验性釉质脱矿在不同材料处理后对牙釉质再矿化影响的研究。方法: 收集因正畸拔除的新鲜前磨牙,随机分为脱矿组(A组);蒸馏水处理组(B组);渗透树脂处理组(C组)。所有离体牙分为近远中釉质块,全部浸泡于雪碧中建立脱矿模型,之后A组釉质面涂布抗酸性指甲油,B组和C组釉质面分别用蒸馏水和渗透树脂处理,所有釉质块置于人工唾液中37℃恒温水浴再矿化8周。每组各随机选取6个近中釉质块,扫描电镜下观察釉质表面的微观结构;其余近中釉质块经处理后,激光共聚焦扫描显微镜下观察荧光图像;远中釉质块采用显微硬度仪检测各组的硬度值。结果: 扫描电镜观察,A组釉质面粗糙,大量釉柱断裂形成大小不等的孔隙样结构,B组可见少量矿物质沉积,部分孔隙变浅甚至消失,C组釉质面较光滑平坦,无明显孔隙。激光共聚焦扫描显微镜观察,A组荧光染料沉积多,荧光面积和荧光强度大,B组和C组荧光染料沉积减少,荧光面积和荧光强度减弱,且C组减弱程度较B组更为明显。显微硬度仪检测结果,B组和C组药物处理后显微硬度值较脱矿后显微硬度值增大,且C组较B组增大幅度更高,差异均具有统计学意义(P<0.05)。结论: 渗透树脂对碳酸饮料造成的实验性釉质脱矿最具有积极的治疗作用。

关键词: 渗透树脂, 碳酸饮料, 牙釉质脱矿, 再矿化

Abstract: Objective: To observe the effects of different materials on remineralization of demineralized enamel caused by carbonated beverage. Methods: Extracted premolars for orthodontic purpose were randomly divided into demineralized group (group A), distilled water treatment group (group B), and penetrating resin treatment group (group C). All teeth were divided into mesial and distal enamel blocks, which were soaked in sprite to establish the demineralization model. Then, the enamel surfaces of group A were covered with acid-resistant nail polish, and the enamel surfaces of group B and group C were treated with distilled water and penetrating resin, respectively. All enamel blocks were placed in artificial saliva for remineralization at 37℃ room temperature water bath for 8 weeks. Six mesial enamel blocks were respectively randomly selected in the A&B&C group. The microstructures of the enamel surface were observed under scanning electron microscope. The fluorescence images of remaining mesial enamel blocks were taken under the laser scanning confocal microscope. The micro-Vickers hardness test was used to evaluate the hardness of distal enamel blocks. Results: Under scanning electron microscope, the enamel surfaces of group A were rough and unequal size pore structure formed by a large number of fractured enamel rod. Enamel surfaces of group B showed a small amount of mineral deposition, and some pores became shallow or disappeared. Enamel surfaces of group C were smooth with no obvious pores. Under the laser scanning confocal microscope, the fluorescence area and intensity of group A were strong, and the fluorescence area and intensity of group B and group C were decreased, and the degree of attenuation in group C was more obvious than that in group B. The microhardness value of group B and group C was higher than that of before remineralization, and the increased value of group C was higher than that of group B (P<0.05). Conclusion: Penetrating resin has a positive therapeutic effect on experimental and demineralized enamel caused by carbonated beverage.

Key words: Penetrating resin, Carbonated beverage , Demineralized enamel , Remineralization

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