雌激素微环境下非经典Wnt/Ca2+信号通路调控人牙周膜干细胞成骨分化的研究

doi:10.13701/j.cnki.kqyxyj.2019.01.008

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (1): 33-37.DOI: 10.13701/j.cnki.kqyxyj.2019.01.008

雌激素微环境下非经典Wnt/Ca²⁺信号通路调控人牙周膜干细胞成骨分化的研究

吴晓玲^1,2, 郑茜^1,2, 吕佳岭^1,2, 赵娴^1,2, 徐洁^1,2, 余京泓^1,2, 徐晓梅^1,2*

1. 西南医科大学附属口腔医院正畸科四川泸州 646000;
2. 西南医科大学口颌面修复重建和再生实验室四川泸州 646000

收稿日期:2018-06-29 出版日期:2019-01-18 发布日期:2019-01-28
通讯作者: 徐晓梅,E-mail:xuxiaomei@hotmail.com
作者简介:吴晓玲(1991~ ),女,四川什邡人,硕士在读,主要从事口腔正畸临床及细胞分子生物学研究。
基金资助:
四川省教育厅项目(重点项目:16ZA0188);四川省科技厅科技计划项目(编号:2015JY0199);四川省科技厅-泸州市-泸州医学院联合项目(编号:LZ-LY-59);泸州市-西南医大联合项目(编号:2016LZXNYD-T04)

Regulation of Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Non-canonical Wnt/Ca²⁺ Signaling Pathway within Estrogen Microenvironment

WU Xiao-ling^1,2, ZHENG Qian^1,2, LV Jia-ling^1,2, ZHAO Xian^1,2, XU Jie^1,2, YU Jing-hong^1,2, XU Xiao-mei^1,2*

1. Department of Orthodontics, Stomatology Hospital of Southwest Medical University, Luzhou 646000, China;
2. Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou 646000, China

Received:2018-06-29 Online:2019-01-18 Published:2019-01-28

摘要/Abstract

摘要： 目的:探讨雌激素作用下非经典Wnt/Ca²⁺信号通路对人牙周膜干细胞( human periodontal ligament stem cells hPDLSCs)成骨分化的调控。方法:将第3代hPDLSCs分为空白组、对照组、雌激素组、抑制剂组,成骨诱导7 d检测ALP活性,Real-time PCR检测各组Wnt信号通路基因及成骨基因表达水平。结果:ALP检测结果示对照组较空白组ALP活性增加(P<0.05),雌激素组和抑制剂组较对照组ALP活性增加(P<0.05),但雌激素组和抑制剂组ALP活性差异无统计学意义(P>0.05)。Real-time PCR检测结果显示对照组较空白组Wnt信号通路基因β-catenin、CaMKⅡ、NLK及成骨基因RUNX2、OCN表达增加(P<0.05),雌激素组和抑制剂组较对照组Wnt信号通路基因β-catenin、CaMKⅡ、NLK及成骨基因RUNX2、OCN表达增加(P<0.05);抑制剂组较雌激素组β-catenin表达下降(P<0.05),而CaMKⅡ、NLK表达增加(P<0.05),但两组成骨基因RUNX2、OCN表达差异无统计学意义(P>0.05)。结论:雌激素可能通过增强非经典Wnt/Ca²⁺信号通路的活化促进hPDLSCs的成骨分化。

关键词: hPDLSCs, 雌激素, 非经典Wnt/Ca²⁺信号通路, 成骨分化

Abstract: Objective: To investigate the effects of estrogen on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) regulated by non-canonical Wnt/Ca²⁺ signaling pathway. Methods: The third generation of hPDLSCs were divided into blank group, control group, estrogen group, and inhibitor group. ALP activity was measured on the 7th day after osteogenesis induction, the expression of Wnt signaling pathway genes and osteoblast-related genes were detected by qRT-PCR. Results: ALP active in estrogen group and inhibitor group were higher than that in control group (P<0.05). There was no significant difference in ALP expression between estrogen group and inhibitor group (P>0.05). qRT-PCR showed that the expression of Wnt signaling pathway genes, such as β-catenin, CaMKⅡ, and NLK, and osteogenic genes (RUNX2, OCN) in estrogen group and inhibitor group were up-regulated compared with control group (P<0.05). The expression of β -catenin in inhibitor group was lower than that in estrogen group (P<0.05), while the expression of CaMKⅡ and NLK was higher (P<0.05). But osteogenic gene RUNX2 and OCN did not exhibit such difference (P>0.05). Conclusion: Estrogen promotes the osteogenic differentiation of hPDLSCs by enhancing the activation of non-canonical Wnt/Ca²⁺ signaling pathway.

Key words: Human periodontal ligament stem cells, Estrogen, Non-canonical Wnt/Ca²⁺ signaling pathway, Osteogenic differentiation

吴晓玲, 郑茜, 吕佳岭, 赵娴, 徐洁, 余京泓, 徐晓梅. 雌激素微环境下非经典Wnt/Ca²⁺信号通路调控人牙周膜干细胞成骨分化的研究[J]. 口腔医学研究, 2019, 35(1): 33-37.

WU Xiao-ling, ZHENG Qian, LV Jia-ling, ZHAO Xian, XU Jie, YU Jing-hong, XU Xiao-mei. Regulation of Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Non-canonical Wnt/Ca²⁺ Signaling Pathway within Estrogen Microenvironment[J]. Journal of Oral Science Research, 2019, 35(1): 33-37.

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雌激素微环境下非经典Wnt/Ca²⁺信号通路调控人牙周膜干细胞成骨分化的研究

Regulation of Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Non-canonical Wnt/Ca²⁺ Signaling Pathway within Estrogen Microenvironment

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