口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (5): 483-487.DOI: 10.13701/j.cnki.kqyxyj.2019.05.017

• 口腔颌面外科损伤修复研究 • 上一篇    下一篇

TGF-β1基因修饰骨髓基质细胞复合Bio-Oss胶原修复犬颌骨缺损的效果

郭宏亮1, 郑纪伟2*, 王鹏来3, 刘宗响3, 袁长永3, 李敢3, 李晓飞3, 耿晓庆3   

  1. 1. 徐州医科大学附属口腔医院修复科 江苏 徐州 221002;
    2. 徐州医科大学口腔医学院 江苏 徐州 221000;
    3. 徐州医科大学附属口腔医院种植中心 江苏 徐州 221002
  • 收稿日期:2018-11-13 出版日期:2019-05-28 发布日期:2019-05-21
  • 通讯作者: 郑纪伟,E-mail:zhengkouqiang@163.com
  • 作者简介:郭宏亮(1976~ ),男,陕西西安人,硕士,副主任医师,研究方向:口腔种植修复及成骨。
  • 基金资助:
    江苏省“六大人才高峰”资助项目(编号:2014-wsw-068)
    江苏省高校自然科学研究计划面上项目(编号:12KJB320015)
    徐州市科技计划项目(编号:KC15SH015)

Effect of TGF-β1 Gene Modified Bone Marrow Stromal Cells Combined with Bio-Oss Collagen on Repairing Canine Jaw Defects.

GUO Hong-liang1, ZHENG Ji-wei2*, WANG Peng-lai3, LIU Zong-xiang3,YUAN Chang-yong3, LI Gan3, LI Xiao-fei3, GENG Xiao-qing3   

  1. 1. Department of Prosthodontics, Affiliated Stomatological Hospital of Xuzhou Medical University, Xuzhou 221002, China;
    2. Xuzhou Medical University, School of Stomatology. Xuzhou 221002, China;
    3. Implant Center, Affiliated Stomatological Hospital of Xuzhou Medical University, Xuzhou 221002, China.
  • Received:2018-11-13 Online:2019-05-28 Published:2019-05-21

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摘要： 目的: 观察TGF-β1基因修饰骨髓基质细胞(BMSC)复合Bio-Oss胶原修复Beagle犬颌骨缺损的效果。方法: Beagle犬18条,随机分为3组:种植体+犬BMSC+凝胶+Bio-Oss胶原材料(对照组);种植体+0.5 μg/L TGF-β1基因转染犬BMSC+凝胶+Bio-Oss胶原材料(实验组A);种植体+10μg/L TGF-β1基因转染犬BMSC+凝胶+Bio-Oss胶原材料组(实验组B)。术后24周取材,组织观察缺损区恢复情况。RT-PCR检测细胞中OC、BSP及COL-Ⅰ mRNA的表达情况。Western Blot检测细胞中OC、BSP及COL-Ⅰ蛋白的表达情况。结果: HE染色结果显示实验组A骨连续性及成熟度优于实验组B。RT-PCR结果显示实验组A三种mRNA表达量明显高于对照组,差异具有统计学意义(P<0.05)。实验组B中3种mRNA表达量与对照组相比,不具有统计学意义(P>0.05)。此外,Western Blot结果中实验组A的蛋白表达量与对照组和实验组B相比明显增加,具有统计学意义(P<0.05),实验组B较对照组略有增加,但差异不具有统计学意义(P＞0.05)。结论: 低浓度的TGF-β1基因可较好地促进BMSC向成骨分化。

关键词: TGF-β1基因, 骨髓基质细胞, Bio-Oss胶原, 基因蛋白表达

Abstract: Objective: To observe the effect of TGF-β1 gene modified Bone Marrow Stromal Cells (BMSC) combined with Bio-Oss collagen on jaw defects reconstruction. Methods: 18 beagle dogs were randomly divided into three groups. Control group: implant+BMSC+gelatin+Bio-Oss collagen group A: implant+BMSC transfected with 0.5 μg/L TGF-β1+gelatin+Bio-Oss collagen; group B: implant+BMSC transfected with 10 μg/L TGF-β1+gelatin+Bio-Oss collagen. Histological view was applied to measure the repair effect. Meanwhile, the mRNA and protein expressions of OC, BSP and COL-I were detected through RT-PCR and Western blot, respectively. Results: HE staining showed that bone continuity and maturity in group A were superior to those in group B. RT-PCR results showed that the expressions of mRNA in the experimental group A were significantly higher than those in the control group (P<0.05). However, compared with the control group, the mRNA expression levels in the experimental group B were not statistically significant (P>0.05). In addition, Western blot results showed that expressions of OC, BSP, and COL-I protein in the group A were significantly higher than those of control group and group B (P<0.05), while that of group B was slightly higher than that of control group, but without statistically significant (P>0.05). Conclusion: 0.5 μg/L TGF-β1 gene has a better repair effect on promoting osteogenic differentiation of BMSC.

Key words: TGF-β1 gene, Bone marrow stromal cells, Bio-Oss collagen, Gene and protein expression