口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (9): 872-875.DOI: 10.13701/j.cnki.kqyxyj.2019.09.013

• 龋病牙髓病学研究 • 上一篇    下一篇

慢性根尖周炎对大鼠血浆炎症因子及主动脉的影响

马典福1,3, 陈帅2,3#, 肖素丽3,4, 李萍萍3, 黄晓晶2,3*   

  1. 1. 厦门市儿童医院口腔科 福建 厦门 361006;
    2. 福建医科大学附属口腔医院 福建 福州 350002;
    3. 福建省高校口腔医学重点实验室 福建 福州 350002;
    4. 厦门市口腔医院 福建 厦门 361000
  • 收稿日期:2019-03-30 出版日期:2019-09-28 发布日期:2019-09-25
  • 通讯作者: 黄晓晶,E-mail:hxiaoj@163.com
  • 作者简介:马典福(1981~ ),男,福建人,硕士,主治医师,研究方向:慢性根尖周炎与全身性疾病的关系。陈帅(1985~ ),男,福建人,博士,主治医师,研究方向:龋病病因学和口腔疾病与全身病关系相关研究。
  • 基金资助:
    国家自然科学基金(编号:81500845、81271131);福建省自然科学基金(编号:2017J01521);福建省医学创新课题(编号:No.2018-CX-37);福建省卫生计生委青年科研课题(编号:No.2015-1-64)

Effect of Chronic Apical Periodontitis on Plasma Cytokine and Aorta Intima in Rats

MA Dianfu1,3, CHEN Shuai2,3#, XIAO Suli3,4, LI Pingping3, HUANG Xiaojing2,3*   

  1. 1. Department of Stomatology, Xiamen Children's Hospital, Xiamen 361006, China;
    2. School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China;
    3. Key Laboratory of Stomatology, Fujian Province University, Fuzhou 350002, China;
    4. Xiamen Stomatological Hospital, Xiamen 361000, China
  • Received:2019-03-30 Online:2019-09-28 Published:2019-09-25

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摘要： 目的:研究大鼠根尖周炎慢性感染12周对血浆炎症因子及主动脉的影响。方法:32只雄性SD大鼠分别经普通饮食(Normal Diet,ND组)和高脂饮食(High Fat Diet,HFD组)喂养9周后,根据磨牙开髓数目ND组再分为N0(假手术组)、N6(6颗开髓),HFD组再分为H0、H6组。第24周,各组大鼠心脏采血测量血脂(TC、TG、HDL-C、LDL-C)、炎症因子(CRP、IL-2、IL-6、IL-10),主动脉样本HE和苏丹Ⅳ染色。结果:CRP、IL-2、IL-6、IL-10各组间差异无统计学意义(P>0.05)。HE染色示N0组主动脉内膜光滑,无明显炎症细胞黏附;N6、H0、H6组见少量炎症细胞位于主动脉肌层和外膜。苏丹Ⅳ染色示各组主动脉壁未见明显红染条纹、斑块等。结论:大鼠根尖周炎慢性感染12周时,即使合并高脂血症,血浆炎症因子未见明显异常,主动脉少量炎症细胞浸润。

关键词: 慢性根尖周炎, 炎症因子, 主动脉

Abstract: Objective: To investigate the effect of chronic apical periodontitis (CAP) for 12 weeks on rat plasma cytokines response and lesion of aorta. Methods: 32 male SD rats were randomly divided into high fat diet group and normal diet group. After 9 weeks, according to the teeth number of opening pulp cavity, the rats were divided into four groups (n=8) to build CAP model, namely N0, N6, H0 and H6. After 24 weeks, plasma concentrations of lipid (TC, TG, HDL-C, LDL-C) and inflammation cytokine (CRP, IL-2, IL-6, IL-10) were detected. Histological examinations of aorta were performed by HE staining and sudan Ⅳ staining. Results: There were no significant difference in plasma CRP, IL-2, IL-6 and IL-10 among four groups (P>0.05). Aorta intima was smooth without obvious inflammation cell adhesion in N0 group. A small amount of inflammatory cells were found in muscularis and adventitia among N6, H0 and H6 groups. Conclusion: CAP for 12 weeks on rats did not raise plasma concentration of cytokine, neither did it when combined with hyperlipidemia. CAP infection caused slight inflammatory cells infiltration in rat aorta.

Key words: chronic apical periodontitis, cytokine, aorta