人β-防御素-3对小鼠牙周炎进展的保护作用

doi:10.13701/j.cnki.kqyxyj.2016.09.002

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (9): 897-901.DOI: 10.13701/j.cnki.kqyxyj.2016.09.002

人β-防御素-3对小鼠牙周炎进展的保护作用

李丽丽,陈斌,崔迪,姜苏,闫福华^*

南京大学医学院附属口腔医院牙周科江苏南京 210008

收稿日期:2016-03-14 出版日期:2016-09-26 发布日期:2016-09-26
通讯作者: 闫福华,E-mail:yanfh@nju.edu.cn
作者简介:李丽丽(1988~ ),女,天津市人,硕士,主要从事牙周病相关的研究工作。
基金资助:
国家自然科学基金(编号:81371152)
江苏省自然科学基金(编号:BK20131079)
江苏省临床医学科技专项(编号:BL2013002)

Protective Effect of Human β-defensin-3 on the Progression of Periodontitis

LI Li-li, CHEN Bin, CUI Di, JIANG Su, YAN Fu-hua^*

Nangjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China

Received:2016-03-14 Online:2016-09-26 Published:2016-09-26

摘要/Abstract

摘要： 目的:探索人β-防御素-3在小鼠牙周炎进展中的保护作用。方法:用丝线结扎法建立小鼠牙周炎模型。实验组小鼠腹腔注射人β-防御素-3(100 μg/kg),另选结扎过小鼠和未结扎小鼠各1组,腹腔注射磷酸盐缓冲液为对照。8周后取上颌骨,Micro CT、HE染色、TRAP染色及免疫组化染色,评估人β-防御素-3对牙周炎进展的作用。结果:人β-防御素-3能减少牙槽骨吸收及骨内破骨细胞数量;减轻牙龈上皮棘层增厚、上皮钉突增生、炎症细胞浸润、胶原纤维变性断裂等病理变化,降低牙周组织中IL-6、MCP-1和TNF-α的表达强度。结论:人β-防御素-3能够减轻牙周组织炎症,减少牙周组织破坏,从而减缓牙周炎进展。

关键词: 人β-防御素-3, 牙周炎, 结扎, 炎症因子

Abstract: Objective: To investigate the effect of human β-defensin-3 on the progression of periodontitis. Methods: Periodontitis models in mice were established through ligating the maxillary second molar with 5-0 silk thread. Mice in the experimental group were given human β-defensin-3 intraperitoneally with a dose of 100μg/kg. Mice with or without ligation were given phosphate buffer solution intraperitoneally as the control. Eight weeks later, the maxilla of each mouse was harvested and detected by Micro-CT scanning, HE staining, TRAP staining, and immunostaining. Results: Human β-defensin-3 reduced the alveolar bone loss and the number of osteoclast in alveolar bone. In addition, human β-defensin-3 alleviated the pathologic changes including thickened spinous layer, hyperplastic epithelial spikes, inflammatory cell infiltration, denatured and broken collagen fibers caused by ligation. Furthermore, lower IL-6, MCP-1 and TNF-α expressions were observed in mice treated with human β-defensin-3. Conclusion: Human β-defensin-3 alleviates the inflammation and destruction in periodontal tissue, thus slowing down the progress of periodontitis.

Key words: Human β-defensin-3, Periodontitis, Ligation, Inflammatory cytokine

中图分类号:

R781.4

李丽丽,陈斌,崔迪,姜苏,闫福华. 人β-防御素-3对小鼠牙周炎进展的保护作用[J]. 口腔医学研究, 2016, 32(9): 897-901.

LI Li-li, CHEN Bin, CUI Di, JIANG Su, YAN Fu-hua. Protective Effect of Human β-defensin-3 on the Progression of Periodontitis[J]. Journal of Oral Science Research, 2016, 32(9): 897-901.

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人β-防御素-3对小鼠牙周炎进展的保护作用

Protective Effect of Human β-defensin-3 on the Progression of Periodontitis

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