偏侧咀嚼对大鼠咬肌的影响及IGF-1调节机制探究

doi:10.13701/j.cnki.kqyxyj.2019.11.020

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (11): 1094-1099.DOI: 10.13701/j.cnki.kqyxyj.2019.11.020

偏侧咀嚼对大鼠咬肌的影响及IGF-1调节机制探究

刘思琦, 李颖辉, 刘子洋, 王雯^*, 马文盛^*

河北医科大学口腔医院·口腔医院,正畸科,河北省重点实验室河北石家庄 050017

收稿日期:2018-12-07 出版日期:2019-11-28 发布日期:2019-11-21
通讯作者: 王雯,E-mail: 82115@163.com 马文盛,E-mail: wsma02@sina.com
作者简介:刘思琦(1994~ ),女,河北沧州人,硕士在读,主要从事口腔正畸临床治疗工作。
基金资助:
河北省高等学校科学技术研究青年基金项目(编号:QN2018145) 河北省重点研发计划项目健康医疗与生物医药专项(编号:18277756D)

Effects of Unilateral Chewing on Structure of Deep Masseter Muscle in Rats and Regulation Mechanism of IGF-1

LIU Siqi, LI Yinghui, LIU Ziyang, WANG Wen^*, MA Wensheng^*

Department of Orthodontics, School and Hospital of Stomatology, Hebei Medical University & Hebei Key Laboratory of Stomatology, Shijiazhuang 050017, China

Received:2018-12-07 Online:2019-11-28 Published:2019-11-21

摘要/Abstract

摘要： 目的: 探究偏侧咀嚼对大鼠深层咬肌结构的影响以及胰岛素样生长因子-1(insulin like growth factor-1,IGF-1)在其中的作用。方法: 4周龄雄性Wistar大鼠24只随机分为实验组和对照组,实验组应用单侧戴用咬合板的方法建立偏侧咀嚼模型,建模后1、2、3、4周处死;取深层咬肌,通过冰冻切片苏木精-伊红(hematoxylin-eosin, HE)染色、三磷酸腺苷酶(adenosine triphosphatase,ATPase)染色、辅酶Ⅰ-四氮唑还原酶(nicotinamide adenine dinucleotide,NADH-TR)染色观察深层咬肌结构变化, 实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,Real-time PCR)检测IGF-1及钙调神经磷酸酶(calcineurin,CaN)mRNA表达变化,酶联免疫吸附试验(enzyme-linked immunosorbent assay, ELISA)法检测IGF-1蛋白水平变化,Western blot检测CaN蛋白水平变化。结果: 与同期对照组比较,建模2、3、4周时非偏侧咀嚼侧MHCⅡa型肌纤维比例增多,并高于偏侧咀嚼侧(P<0.05);且IGF-1和CaN的蛋白及mRNA水平在3、4周时高于偏侧咀嚼侧及对照组(P<0.05)。结论: 长期偏侧咀嚼促使非偏侧咀嚼侧咬肌纤维类型由MHCⅡb型向MHCⅡa型转换,这可能与IGF-1及CaN的分泌增多有关。

关键词: 偏侧咀嚼, 咬肌, 胰岛素样生长因子-1, 钙调神经磷酸酶

Abstract: Objective: To investigate the effect of unilateral chewing on the structure of deep masseter in rats and the role of IGF-1. Methods: Twenty-four male Wistar rats aged 4 weeks were randomly divided into experimental group and control group. A unilateral chewing model was established by unilateral occlusal plate. After 1, 2, 3, and 4 weeks of modeling, the deep masseter muscle was taken and the structural changes were observed by frozen section HE, ATPase, and NADH-TR staining. The expression of IGF-1 and CaN mRNA was detected by real-time RCR. The level of IGF-1 protein was detected by ELISA and the CaN protein was detected by Western blot. Results: After 2, 3, and 4 weeks of wearing occlusal plate, the proportion of MHCⅡa fibers on the non-biased chewing side increased more than that of the chewing side and was higher than that of the control group (P<0.05). The protein and mRNA content of IGF-1 and CaN on the non-biased chewing side were higher than that of the non-biased chewing side and control group at week 3 and 4 (P<0.05). Conclusion: Unilateral chewing leads to the transition of muscle fiber type from MHCⅡb to MHCⅡa, which may relate to the increased secretion of IGF-1 and CaN.

Key words: unilateral chewing, masseter, insulin-like growth factor-1, calcineurin

刘思琦, 李颖辉, 刘子洋, 王雯, 马文盛. 偏侧咀嚼对大鼠咬肌的影响及IGF-1调节机制探究[J]. 口腔医学研究, 2019, 35(11): 1094-1099.

LIU Siqi, LI Yinghui, LIU Ziyang, WANG Wen, MA Wensheng. Effects of Unilateral Chewing on Structure of Deep Masseter Muscle in Rats and Regulation Mechanism of IGF-1[J]. Journal of Oral Science Research, 2019, 35(11): 1094-1099.

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偏侧咀嚼对大鼠咬肌的影响及IGF-1调节机制探究

Effects of Unilateral Chewing on Structure of Deep Masseter Muscle in Rats and Regulation Mechanism of IGF-1

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