牵张力作用下小鼠颅骨缝成骨的定量分析

doi:10.13701/j.cnki.kqyxyj.2020.08.012

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (8): 753-756.DOI: 10.13701/j.cnki.kqyxyj.2020.08.012

牵张力作用下小鼠颅骨缝成骨的定量分析

王珈璐, 张卫兵^*

南京医科大学附属口腔医院正畸科,江苏省口腔疾病重点研究室江苏南京 210000

收稿日期:2019-12-31 出版日期:2020-08-28 发布日期:2020-08-18
通讯作者: *张卫兵,E-mail: weibingzhang@njmu.edu.cn
作者简介:王珈璐(1994~ ),女,江苏宜兴人,硕士在读,主要从事口腔正畸临床诊疗工作。
基金资助:
国家自然科学基金面上项目(编号:81671019)

Quantitative Analysis of Cranial Suture Distraction Osteogenesis in Mice

WANG Jialu, ZHANG Weibing^*

Jiangsu Key Laboratory of Oral Diseases, Department of Orthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210000, China

Received:2019-12-31 Online:2020-08-28 Published:2020-08-18

摘要/Abstract

摘要： 目的: 利用Micro CT(micro computed tomography)定量分析小鼠颅骨缝牵张成骨新骨形成。方法: 选取30只4~5周龄健康C57BL/6J小鼠,随机分为颅骨矢状缝假手术对照组与牵张加力组,每组15只。每组随机分为3小组,每小组5只,分别在牵张7、14、28 d后处死。对颅骨进行Micro CT扫描和苏木精-伊红(hematoxylin-eosin, HE)染色分析。结果: 与同期对照组比较,牵张加力组小鼠矢状缝平均扩大(3.55±0.53) mm(P<0.05),Micro CT和HE染色证明牵张区有新骨形成,形成部位主要位于顶骨边缘。结论: Micro CT可以直观还原小鼠颅骨缝牵张成骨过程并对骨骼参数进行全面定量分析,对于研究骨缝牵张成骨应用前景广阔。

关键词: 颅骨缝, 牵张成骨, 定量分析

Abstract: Objective: To observe cranial suture distraction osteogenesis process quantitatively and to lay the foundation for further research. Methods: 30 healthy C57BL/6J mice aged 4-5 weeks were randomly divided into sham operation group and expansion group (n=15 per group). Mice in each group were sacrificed at 7, 14, and 28 days after stretch. Newly formed bone was analyzed by Micro CT scan and histological staining. Results: Sagittal sutures in expansion group were enlarged by (3.55±0.53) mm averagely(P<0.05). Micro CT and histological analysis showed that there was new bone formation in the distraction area, which sited mostly at edge of parietal bones. Conclusion: The cranial suture distraction osteogenesis could be directly restored. Bone parameters could be comprehensively and quantitatively analyzed using Micro CT, which has a broad application prospect for the study of suture distraction osteogenesis.

Key words: cranial suture, distraction osteogenesis, quantitative analysis

王珈璐, 张卫兵. 牵张力作用下小鼠颅骨缝成骨的定量分析[J]. 口腔医学研究, 2020, 36(8): 753-756.

WANG Jialu, ZHANG Weibing. Quantitative Analysis of Cranial Suture Distraction Osteogenesis in Mice[J]. Journal of Oral Science Research, 2020, 36(8): 753-756.

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牵张力作用下小鼠颅骨缝成骨的定量分析

Quantitative Analysis of Cranial Suture Distraction Osteogenesis in Mice

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