口腔医学研究

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (12): 1137-1141.DOI: 10.13701/j.cnki.kqyxyj.2020.12.013

• 牙髓病学研究 • 上一篇    下一篇

生物活性玻璃促进牙本质再矿化的时间相关性体外研究

岳阳丽*, 马贵霞, 刘翠丽, 贺贵天   

  1. 郑州大学第一附属医院,郑州大学口腔医学院 河南 郑州 450052
  • 收稿日期:2020-04-10 出版日期:2020-12-28 发布日期:2020-12-28
  • 通讯作者: *岳阳丽,E-mail:yueyangli@zzu.edu.cn
  • 作者简介:岳阳丽(1975~ ),女,副主任医师,硕士,研究方向:牙体硬组织缺损与修复。
  • 基金资助:
    河南省科技厅科技攻关项目(编号:162102310129)

In Vitro Study on Time Correlation of Bioactive Glass in Promoting Dentin Remineralization

YUE Yangli*, MA Guixia, LIU Cuili, HE Guitian   

  1. The First Affiliated Hospital, College of Stomatology, Zhengzhou University, Zhengzhou 450052, China
  • Received:2020-04-10 Online:2020-12-28 Published:2020-12-28

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摘要： 目的:研究生物活性玻璃(Bioactive glass,BAG)不同作用时间下促进牙本质再矿化的效果。方法:采用0.5 mol/L EDTA制备完全脱矿牙本质盘,按BAG处理时间和方式不同随机分为5组:A组(1 d)、B组(4 d)、C组(7 d)、D组(14 d)和空白对照组E组,用聚焦离子束扫描电镜(focused ion beam scanning electron microscopy,FIB SEM)、X射线能谱分析仪(energy dispersive X-ray,EDX)、X射线衍射仪(X-ray diffractometer,XRD)、傅里叶变换衰减全反射红外光谱仪(attenuated total reflection fourier transform infrared spectrometer,ATR-FTIR)检测牙本质表面形态及矿物质的理化性质。结果:FIB SEM示BAG处理时间越长,形成的矿物质越致密,牙本质小管阻塞程度越高;EDX示D组的钙磷含量及钙磷比显著高于A、B、C三组,差异有统计学意义(P<0.05);ATR-FTIR示BAG处理时间越长,磷酸根的吸收峰强度越高;XRD示B、C、D组形成了类似羟基磷灰石的衍射峰。结论:BAG能促进完全脱矿的牙本质形成类似羟基磷灰石结构而发生再矿化,且具有时间累积效应。

关键词: 生物活性玻璃, 再矿化, 时间

Abstract: Objective: To study the effect of bioactive glass on promoting dentin remineralization at different time points. Methods: 0.5mol/L EDTA was used to prepare completely demineralized dentin plates, which were randomly divided into 5 groups according to different treatment time points: experimental group A (1 day), group B (4 days), group C (7 days), group D (14 days), and blank control group E. The dentin morphology and the physicochemical properties of minerals were detected by focused ion beam scanning electron microscopy (FIB SEM), energy dispersive X-ray (EDX), X-ray diffractometer (XRD), and attenuated total reflection fourier transform infrared spectrometerATR-FTIR). Results: FIB SEM showed that the longer the treatment time was, the denser the minerals and the higher the degree of occlusion of dentin tubules were. EDX showed that the calcium-phosphorus content and the calcium-phosphorus ratio in group D were significantly higher than those in group A, B, and C (P<0.05). ATR-FTIR showed that the longer the treatment time was, the higher the absorption peak strength of phosphate was. And XRD showed that groups B, C, and D had formed diffraction peaks similar to hydroxyapatite. Conclusion: BAG can promote the completely demineralized dentin to form minerals similar to hydroxyapatite structure, which has accumulated effect as time proceeded.

Key words: bioactive glass, remineralization, time