USP 12促进循环牵张力作用下人牙周膜干细胞的成骨分化过程

doi:10.13701/j.cnki.kqyxyj.2021.06.011

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (6): 528-532.DOI: 10.13701/j.cnki.kqyxyj.2021.06.011

USP 12促进循环牵张力作用下人牙周膜干细胞的成骨分化过程

邹浩^1,2, 常茂琳^1,2, 张珍^1,2, 韩光丽^2*

1.武汉大学口腔医学院口腔基础医学省部共建国家重点实验室培训基地和口腔生物学教育部重点实验室湖北武汉 430079;
2.武汉大学口腔医院正畸二科湖北武汉 430079

收稿日期:2021-02-25 出版日期:2021-06-28 发布日期:2021-06-17
通讯作者: *韩光丽,E-mail:guanglihan@whu.edu.cn
作者简介:邹浩(1996~ ),男,湖北监利人,硕士在读,主要从事口腔正畸学研究。
基金资助:
国家自然科学基金(编号:81771111)

USP 12 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells under Cyclic Tension

ZOU Hao^1,2, CHANG Maolin^1,2, ZHANG Zhen^1,2, HAN Guangli^2*

1. The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, Wuhan 430079, China;
2. Department of Orthodontics(Division 2), School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China

Received:2021-02-25 Online:2021-06-28 Published:2021-06-17

摘要/Abstract

摘要： 目的:探究泛素特异性蛋白酶12(USP 12)在循环牵张力刺激引起的人牙周膜干细胞(hPDLSCs)成骨分化中的作用。方法:原代培养健康的hPDLSCs,通过成骨、成脂及成软骨诱导分化实验检测其干细胞分化特性。使用循环牵张力刺激hPDLSCs 24 h、48 h、72 h,实时定量PCR、免疫印迹和免疫荧光实验检测USP 12的表达变化,ALP染色检测hPDLSCs的成骨分化,实时定量PCR检测成骨相关因子ALP、OGT、OPN的表达变化。结果:诱导分化试验染色显示本研究使用的原代hPDLSCs具有多向分化潜力。循环牵张力刺激hPDLSCs后, USP 12的表达均呈先升高后降低的趋势。在循环牵张力刺激下hPDLSCs发生成骨分化,ALP、OGT的表达先升高后降低,OPN逐渐升高。结论:USP 12可能促进循环牵张力作用下人牙周膜干细胞的成骨分化。

关键词: 循环牵张力, 人牙周膜干细胞, 骨形成, 泛素特异性蛋白酶12

Abstract: Objective: To investigate the influence of ubiquitin-specific protease 12 (USP 12) on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) under cyclic tension. Methods: Primary hPDLSCs were collected and the differentiation characteristics were detected by osteogenic, adipogenic, and chondrogenic differentiation experiments. The expression of USP 12 was detected by real-time quantitative PCR, Western blot, and immunofluorescence assay. The osteogenic differentiation of hPDLSCs was detected by ALP staining test. The expressions of ALP, OGT, and OPN were detected by real-time quantitative PCR. Results: The primary hPDLSCs had the potential of multi-directional differentiation. The expression of USP 12 increased at first and then decreased after cyclic stretch stimulation. The expression of ALP and OGT increased at first and then decreased, and the expression of OPN increased gradually. Conclusion: USP 12 may promote osteogenic differentiation of hPDLSCs under cyclic tension.

Key words: cyclic tension, human periodontal ligament stem cell, osteogenesis, ubiquitin-specific protease 12

邹浩, 常茂琳, 张珍, 韩光丽. USP 12促进循环牵张力作用下人牙周膜干细胞的成骨分化过程[J]. 口腔医学研究, 2021, 37(6): 528-532.

ZOU Hao, CHANG Maolin, ZHANG Zhen, HAN Guangli. USP 12 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells under Cyclic Tension[J]. Journal of Oral Science Research, 2021, 37(6): 528-532.

参考文献

[1] 邬扬绚,向黎,黄志强,等.釉基质蛋白衍生物调节牙龈卟啉单胞菌感染牙周膜干细胞成骨分化的作用及机制[J].口腔医学研究,2020,36(6):534-538.
[2] Yang Y, Wang BK, Chang ML, et al. Cyclic stretch enhances osteogenic differentiation of human periodontal ligament cells via YAP activation [J]. Biomed Res Int, 2018, 2018:2174824.
[3] Chang M, Lin H, Luo M, et al. Integrated miRNA and mRNA expression profiling of tension force-induced bone formation in periodontal ligament cells [J]. In Vitro Cell Dev Biol Anim, 2015, 51(8):797-807.
[4] Zong Z, Zhang Z, Wu L, et al. The functional deubiquitinating enzymes in control of innate antiviral immunity [J]. Adv Sci (Weinh), 202, 8(2):2002484.
[5] Chang M, Lin H, Fu H, et al. CREB activation affects mesenchymal stem cell migration and differentiation in periodontal tissues due to orthodontic force [J]. Int J Biochem Cell Biol, 2020, 129:105862.
[6] Yu H, Yu W, Liu Y, et al. Expression of HIF 1α in cycling stretch induced osteogenic differentiation of bone mesenchymal stem cells [J]. Mol Med Rep, 2019, 20(5):4489-4498.
[7] 孙超.葡萄糖诱导Sox9/Runx2 O-GlcNAc糖基化在软骨终板干细胞分化中的作用及机制研究[D].重庆:中国人民解放军陆军军医大学,2020.
[8] 张琳琳,安莹,陈发明,等.牙源性干细胞的研究进展[J].实用口腔医学杂志,2015,31(3):425-431.
[9] 李汝磊.ETS2对牙周膜干细胞成骨分化的影响[D].重庆:中国人民解放军陆军军医大学,2019.
[10] Liu S, de Boeck M, van Dam H, et al. Regulation of the TGF-β pathway by deubiquitinases in cancer [J]. Int J Biochem Cell Biol, 2016, 76:135-145.
[11] Guo YC, Wang MY, Zhang SW, et al. Ubiquitin-specific protease USP34 controls osteogenic differentiation and bone formation by regulating BMP2 signaling [J]. EMBO J, 2018, 37(20):e99398.
[12] Williams S, Maecker H, French D, et al. USP1 deubiquitinates ID proteins to preserve a mesenchymal stem cell program in osteosarcoma [J]. Cell, 2011, 146(6):918-930.
[13] Zhou F, Li F, Fang P, et al. Ubiquitin-specific protease 4 antagonizes osteoblast differentiation through dishevelled [J]. J Bone Miner Res, 2016, 31(10):1888-1898.
[14] Lau AW, Pringle LM, Quick L, et al. TRE17/ubiquitin-specific protease 6 (USP6) oncogene translocated in aneurysmal bone cyst blocks osteoblastic maturation via an autocrine mechanism involving bone morphogenetic protein dysregulation[J]. J Biol Chem, 2010, 285(47):37111-37120.
[15] Aron R, Pellegrini P, Green EW, et al. Deubiquitinase Usp12 functions noncatalytically to induce autophagy and confer neuroprotection in models of Huntington's disease [J]. Nat Commun, 2020, 11(1):2374.
[16] Joo HY, Jones A, Yang C, et al. Regulation of Histone H2A and H2B Deubiquitination and Xenopus Development by USP12 and USP46 [J]. J Biol Chem, 2011, 286(9):7190-7201.
[17] Nayak TKS , Alamuru-Yellapragada NP , Parsa KVL . Deubiquitinase USP12 promotes LPS induced macrophage responses through inhibition of IκBα [J]. Biochem Biophys Res Commun, 2017, 483(1):69-74.

USP 12促进循环牵张力作用下人牙周膜干细胞的成骨分化过程

USP 12 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells under Cyclic Tension

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