SRSF3调控口腔癌细胞中SPAG5外显子3的可变剪切的研究

doi:10.11839/kqyxzh.2016.01.00

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (1): 8-11.DOI: 10.11839/kqyxzh.2016.01.00

SRSF3调控口腔癌细胞中SPAG5外显子3的可变剪切的研究

刘佩琦,石黎明,贾荣^*

武汉大学口腔医学院·湖北省口腔基础医学重点实验室-省部共建国家重点实验室培育基地·
武汉大学口腔生物医学教育部重点实验室湖北武汉 430079

收稿日期:2015-07-29 出版日期:2016-01-28 发布日期:2016-03-21
通讯作者: 贾荣,电话:027-87686268
作者简介:刘佩琦(1991~),女,河南省洛阳市人,硕士在读,主要从事口腔内科学的研究工作。

Alternative Splicing of SPAG5 Exon3 is Regulated by SRSF3 in Oral Squamous Cell Carcinoma.

LIU Pei-qi, SHI Li-ming, JIA Rong.

The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China

Received:2015-07-29 Online:2016-01-28 Published:2016-03-21

摘要/Abstract

摘要： 目的:研究调控前体mRNA(pre-mRNA)可变剪切的原癌基因SRSF3对细胞有丝分裂的重要功能蛋白SPAG5的外显子3的可变剪切的调控作用。方法:在人口腔鳞状上皮细胞癌细胞系CAL 27中应用RNAi技术降低SRSF3的表达,通过逆转录PCR(RT-PCR)检测SPAG5外显子3的可变剪切。结果:在CAL 27细胞中抑制SRSF3表达时,抑制了全长的SPAG5的表达,增加了较短的SPAG5的剪切异构体的表达。结论:SRSF3可以调控SPAG5外显子3的可变剪切,并可能影响了SPAG5蛋白的表达水平和有丝分裂。

关键词: SRSF3, SPAG5, 可变剪切, 有丝分裂

Abstract: Objective: To study the effect of SRSF3 on alternative splicing of SPAG5 exon3 in oral squamous cell carcinoma (OSCC). Methods: SRSF3 was knocked down using siRNA in OSCC CAL27 cells. Alternative splicing of SPAG5 exon3 was analyzed by RT-PCR. Results: Knockdown of SRSF3 inhibited the expression of full length SPAG5 and increased short spliced product of SPAG5 in CAL27 cells. Conclusion: SRSF3 could down-regulate the expression of SPAG5 protein and interfere with mitosis in OSCC.

中图分类号:

R739.8

刘佩琦,石黎明,贾荣. SRSF3调控口腔癌细胞中SPAG5外显子3的可变剪切的研究[J]. 口腔医学研究, 2016, 32(1): 8-11.

LIU Pei-qi, SHI Li-ming, JIA Rong.. Alternative Splicing of SPAG5 Exon3 is Regulated by SRSF3 in Oral Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2016, 32(1): 8-11.

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SRSF3调控口腔癌细胞中SPAG5外显子3的可变剪切的研究

Alternative Splicing of SPAG5 Exon3 is Regulated by SRSF3 in Oral Squamous Cell Carcinoma.

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