HnRNP L调控口腔癌细胞中CD55内含子7的可变剪切的研究

doi:10.13701/j.cnki.kqyxyj.2016.10.002

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (10): 1006-1009.DOI: 10.13701/j.cnki.kqyxyj.2016.10.002

HnRNP L调控口腔癌细胞中CD55内含子7的可变剪切的研究

张偲¹, 刘苗苗¹, 刘佩琦¹, 贾荣^1*, 郭继华^1,2*

1. 武汉大学口腔医学院,湖北省口腔基础医学重点实验室-省部共建国家重点实验室培育基地,口腔生物医学教育部重点实验室武汉大学湖北武汉 430079;
2. 武汉大学口腔医院牙体牙髓科湖北武汉 430079

收稿日期:2016-02-14 出版日期:2016-10-26 发布日期:2016-10-26
通讯作者: 贾荣,电话:027-87686268;郭继华,电话:027-87686208
作者简介:张偲(1990~ ),女,湖南人,硕士,主要从事牙体牙髓病学相关基础研究和临床工作。
基金资助:
国家自然科学基金(编号:81470741)

Alternative Splicing of CD55 Intron 7 Is Regulated by HnRNP L in Oral Squamous Cell Carcinoma.

ZHANG Si¹, LIU Miao-miao¹, LIU Pei-qi¹, JIA Rong^1*, GUO Ji-hua^1,2*.

1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
2.Department of Endodontics, Hubei-MOST KLOS & KLOBME, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.

Received:2016-02-14 Online:2016-10-26 Published:2016-10-26

摘要/Abstract

摘要： 目的:研究在口腔癌细胞中剪切因子hnRNP L对其新的靶基因CD55的内含子7的可变剪切的调控作用。方法::应用GEO2R在线软件对GEO数据库中的关于hnRNP L沉默后细胞前体mRNA可变剪切改变的外显子阵列数据进行再分析,发现了一系列hnRNP L的新靶基因,然后在人口腔鳞状细胞癌细胞系CAL 27中应用RNA干扰技术降低hnRNP L的表达,通过逆转录PCR检测CD55内含子7的可变剪切。结果:对外显子阵列数据再分析发现CD55是hnRNP L的靶基因。在CAL 27细胞中抑制hnRNP L的表达时,促进了包含内含子7的CD55亚型的表达。结论:CD55内含子7的保留受到hnRNP L的调控。CD55是hnRNP L在口腔癌细胞中的靶基因。

关键词: hnRNP, L, CD55, 可变剪切, 口腔癌

Abstract: Objective: To study the effect of splicing factor hnRNP L on alternative splicing of CD55 intron 7, a new target of hnRNP L in CAL 27 cells. Methods: An exon array data about hnRNP L down-regulation in cells in GEO database was re-analyzed with GEO2R online service. HnRNP L was knocked down by using siRNA in oral squamous cell carcinoma (OSCC) CAL 27 cells. Alternative splicing of CD55 intron 7 was analyzed by RT-PCR. Results: CD55 is a new target of hnRNP L. Knockdown of hnRNP L in CAL 27 cells significantly increased the inclusion of CD55 intron 7.Conclusion: The retention of CD55 intron 7 was regulated by hnRNP L. CD55 was the target of hnRNP L in OSCC cells.

Key words: HnRNP L, CD55, Alternative splicing, OSCC

中图分类号:

R739.8

张偲, 刘苗苗, 刘佩琦, 贾荣, 郭继华. HnRNP L调控口腔癌细胞中CD55内含子7的可变剪切的研究[J]. 口腔医学研究, 2016, 32(10): 1006-1009.

ZHANG Si, LIU Miao-miao, LIU Pei-qi, JIA Rong, GUO Ji-hua.. Alternative Splicing of CD55 Intron 7 Is Regulated by HnRNP L in Oral Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2016, 32(10): 1006-1009.

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HnRNP L调控口腔癌细胞中CD55内含子7的可变剪切的研究

Alternative Splicing of CD55 Intron 7 Is Regulated by HnRNP L in Oral Squamous Cell Carcinoma.

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