E-钙黏素mRNA在大鼠舌黏膜癌变过程中的表达研究

doi:10.11839/kqyxzh.2016.01.008

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (1): 31-34.DOI: 10.11839/kqyxzh.2016.01.008

E-钙黏素mRNA在大鼠舌黏膜癌变过程中的表达研究

谭红^1,2,毛亮³,王敏²,廖鹏程²,聂敏海^1*

1. 四川医科大学口腔医学院四川泸州 646000;
2. 四川医科大学口颌面重建和再生实验室四川泸州 646000;
3. 四川医科大学医学电生理学教育部重点实验室四川泸州 646000

收稿日期:2015-08-13 出版日期:2016-01-28 发布日期:2016-03-21
通讯作者: 聂敏海,E-mail:nieminhai@126.com
作者简介:谭红(1987~),女,四川人,医师,硕士,主要从事口腔内科临床治疗工作。
基金资助:
四川省应用基础研究计划项目(编号:2013JY0126)

Expression of E-cadherin mRNA in the Rat Tongue Carcinogenesis.

TAN Hong^1,2, MAO Liang³, WANG Min², LIAO Peng-cheng², NIE Min-hai¹.

1. Stomatology of Sichuan Medical University, Luzhou 646000, China;
2. Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Sichuan Medical University, Luzhou 646000, China;
3. Key Laboratory of Medical Electrophysiology, Ministry of Education, Sichuan Medical University, Luzhou 646000, China

Received:2015-08-13 Online:2016-01-28 Published:2016-03-21

摘要/Abstract

摘要： 目的:研究上皮型黏附分子E-钙粘素mRNA在大鼠舌黏膜癌变过程中的表达及其意义。方法:采用4NQO饮水法诱导大鼠舌鳞状细胞癌发生,实时荧光定量PCR技术检测组织标本中E-钙粘素mRNA的表达情况。结果:在大鼠舌鳞状细胞癌发生过程中E-钙粘素mRNA表达降低;上皮单纯增生组、轻度上皮异常增生组、中度和重度上皮异常增生组、鳞状细胞癌组4组标本中E-钙粘素 mRNA的表达量分别是上皮正常组标本中的0.453541倍、0.207062倍、0.190954倍、0.180987倍,且鳞状细胞癌组与上皮正常组间的差异具有统计学意义。结论:在大鼠舌黏膜癌变过程中E-钙粘素mRNA表达随着病理分级的增加呈逐渐降低的趋势。E-钙粘素mRNA表达变化是大鼠舌黏膜癌变过程中的早期事件。

关键词: 舌鳞状细胞癌, E-cad, mRNA, q-RT-PC

Abstract: Objective: To investigate the expression and significance of E-cadherin mRNA in the rat tongue carcinogenesis. Methods: 4NQO Drinking Water Act was used to induce tongue squamous cell carcinoma of rat. The mRNA expression level of E-cadherin was analyzed by q-RT-PCR. Results: The mRNA expression of E-cadherin was down-regulated in the rat tongue carcinogenesis. In the epithelial hyperplasia tissues, mild epithelial dysplasia tissues, moderate to severe epithelial dysplasia tissues and squamous cell carcinoma tissues, E-cadherin mRNA level was 0.453541, 0.207062, 0.190954, 0.180987 times by the normal epithelial tissues, respectively. The differences between squamous cell carcinoma group and normal epithelial group were statistically significant (P＜0.05). Conclusion: With the increase of pathological grade, E-cadherin mRNA expression showed a decreasing trend. The change of E-cadherin mRNA expression was an early event in rat tongue mucosa carcinogenesis.

中图分类号:

R739.8

谭红,毛亮,王敏,廖鹏程,聂敏海. E-钙黏素mRNA在大鼠舌黏膜癌变过程中的表达研究[J]. 口腔医学研究, 2016, 32(1): 31-34.

TAN Hong, MAO Liang, WANG Min, LIAO Peng-cheng, NIE Min-hai.. Expression of E-cadherin mRNA in the Rat Tongue Carcinogenesis.[J]. Journal of Oral Science Research, 2016, 32(1): 31-34.

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E-钙黏素mRNA在大鼠舌黏膜癌变过程中的表达研究

Expression of E-cadherin mRNA in the Rat Tongue Carcinogenesis.

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