口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (10): 1122-1126.DOI: 10.13701/j.cnki.kqyxyj.2018.10.022

• 口腔颌面外科学研究 • 上一篇    下一篇

二维及三维培养对人舌鳞状细胞癌TCA8113细胞株生物学特性的影响

周艳萌1*, 朗春媚2, 申航3, 胡芳媛4, 胡铭4, 邓文博4   

  1. 1. 遵义医学院微生物学与免疫学实验室 贵州 遵义 563000;
    2. 遵义医学院医学与科技学院2008级 贵州 遵义 563000;
    3. 遵义医学院医学与科技学院2011级 贵州 遵义 563000;
    4. 遵义医学院2014级 贵州 遵义 563000
  • 收稿日期:2018-04-10 出版日期:2018-10-28 发布日期:2018-10-24
  • 通讯作者: 周艳萌,E-mail:307879470@qq.com
  • 作者简介:周艳萌(1976~ ),女,贵州毕节人,副教授,硕士,主要从事中药抗肿瘤、抗微生物作用的研究。
  • 基金资助:
    贵州省大学生创新性实验计划项目(编号:201413653002),遵义医学院医学与科技学院大学生创新性实验计划项目(编号:遵医科院发[2013] 3901)

Effects of Two-dimensional and Three-dimensional Culture on Biological Characteristics of Human Tongue Squamous Cell Carcinoma TCA8113 Cell Lines.

ZHOU Yan-meng1*, LANG Chun-mei2, SHEN Hang3, HU Fang-yuan4, HU Ming4, DENG Wen-bo4.   

  1. 1. Department of Microbiology and Immunology,Zunyi Medical University, Zunyi 563000, China;
    2. Student of Grade 2008 in the Medicine & Technology School, Zunyi Medical University, Zunyi 563000, China;
    3. Student of Grade 2011 in the Medicine & Technology School, Zunyi Medical University, Zunyi 563000, China;
    4. Student of Grade 2014 in the Department of Clinical Medicine, Zunyi Medical University, Zunyi 563000, China.
  • Received:2018-04-10 Online:2018-10-28 Published:2018-10-24

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摘要： 目的: 比较二维及三维方式培养的人舌鳞状细胞癌(简称鳞癌)TCA8113细胞株对细胞的增殖、组织形态及乳酸脱氢酶(lactate dehydrogenase,LDH)活性的影响,为体外舌鳞癌的研究提供培养方法。方法: 倒置显微镜和苏木精-伊红(hematoxylin and eosin,HE)染色观察二维及三维培养的人舌鳞癌TCA8113细胞的形态;噻唑蓝(methyl thiazolyl tetrazolium,MTT)法观察两种培养体系中细胞的增殖;酶联免疫吸附试验(Enzyme-linked immunosorbent assay,ELISA)检测两种培养体系中LDH活性的改变。结果: 倒置显微镜和HE染色观察到三维培养体系中TCA8113细胞成团生长;与二维培养体系比较,三维培养体系中细胞较长时间保持良好的增殖状态,LDH的活性较二维培养的细胞活性显著增高。结论: TCA8113细胞藻酸盐凝胶三维培养体系较二维培养体系能更好地体现细胞的生物学特性。

关键词: 舌鳞状细胞癌, 培养技术, 藻酸盐凝胶, 乳酸脱氢酶

Abstract: Objective: To compare the effect of 2D and 3D culture on the cell proliferation, organization form, and activity of lactate dehydrogenase of TCA8113 tongue squamous cell carcinoma cell line, and to provide methods for the culture of tongue squamous carcinoma cells in vitro. Methods: The morphology of TCA8113 cells was observed by inverted microscope and HE staining. MTT method was used to observe the proliferation of cells in two types of culture. ELISA was used to detect the changes in LDH activity. Results: Compared with the 2D culture system, the cells in the 3D culture system maintained a good proliferation status in the long period, and the activity of LDH was significantly higher than that of 2D culture. Conclusion: Alginate gel 3D culture system was better than 2D culture system for to reflect the biological characteristics of TCA8113 cells.

Key words: Tongue squamous cell carcinoma, Culture techniques, Alginate gel, Lactate dehydrogenase