口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (12): 1278-1281.DOI: 10.13701/j.cnki.kqyxyj.2017.12.010

• 基础研究论著 • 上一篇    下一篇

YAP1小干扰RNA对舌鳞状细胞癌生物学特性的影响

吴昌敬1,王蔚璐2,甄恩明1,邵军1*   

  1. 1. 广州市中西医结合医院口腔科 广东 广州 510800;
    2. 广州市花都区妇幼保健院胡忠医院 广东 广州 510800
  • 收稿日期:2017-05-11 出版日期:2017-12-20 发布日期:2018-01-03
  • 通讯作者: 邵军,E-mail:1879469320@qq.com
  • 作者简介:吴昌敬(1982~ ),男,江苏省泗阳县人,硕士,主治医师,主要从事口腔临床研究。
  • 基金资助:
    广州市花都区医疗卫生科研基金(编号:15-HDWS-050)

Effect of YAP1 Small Interfering RNA on Biological Characteristics of Tongue Squamous Cell.

WU Chang-jing1, WANG Wei-Lu2, ZHEN En-ming1, SHAO Jun1*.   

  1. 1. Department of Stomatology, Guangzhou Hospital of Integrated Traditional and West Medicine, Guangzhou 510800, China;
    2. Maternal and Child Health Hospital of Huadu District of Guangzhou, Guangzhou 510800, China.
  • Received:2017-05-11 Online:2017-12-20 Published:2018-01-03

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摘要： 目的: 探讨小分子RNA干扰(siRNA)YAP1基因对舌鳞状细胞癌生物学特性的影响。方法: 培养人舌癌SCC-4细胞,根据转染物不同,分为siRNA-YAP1组、siRNA-对照序列组和空白对照组,实时荧光定量PCR技术检测各组细胞中YAP1基因表达,Western blot法检测各组细胞中YAP1蛋白表达,MTT法检测各组细胞增殖能力,流式细胞技术检测各组细胞凋亡情况,划痕实验检测各组细胞迁移能力,Transwell法检测各组细胞侵袭能力。结果: 与siRNA-对照序列组和空白对照组比较,siRNA-YAP1组细胞中YAP1 mRNA和蛋白相对表达量均降低,差异均有统计学意义(P<0.05);与siRNA-对照序列组和空白对照组比较,siRNA-YAP1组细胞24 h、48 h、72 h、96 h时A值均降低,差异均有统计学意义(P<0.05);与siRNA-对照序列组和空白对照组比较,siRNA-YAP1组细胞凋亡率均升高,差异有统计学意义(P<0.05);与siRNA-对照序列组和空白对照组比较,siRNA-YAP1组细胞划痕愈合率降低,差异有统计学意义(P<0.05);与siRNA-对照序列组和空白对照组比较,siRNA-YAP1组侵袭细胞数减少,差异有统计学意义(P<0.05)。结论: 特异性沉默人舌癌SCC-4细胞中YAP1基因可抑制细胞增殖,加速细胞凋亡,减少细胞迁移和侵袭能力。

关键词: 舌鳞状细胞癌, YAP1, 小干扰RNA, 生物学特性

Abstract: Objective: To investigate the effect of YAP1 small interfering RNA (siRNA) on the biological characteristics of tongue squamous cell. Methods: Human tongue cancer SCC-4 cells were cultured. According to the different transfectants, the cells were divided into siRNA-YAP1 group, siRNA-control sequence group, and blank control group. The expression of YAP1 gene in each group was detected by using real-time fluorescence quantitative PCR. The expression of YAP1 protein in each group was detected by Western blot. The cell proliferation was detected by MTT assay. The cell apoptosis in each group was detected by flow cytometry. The cell migration ability was detected by scanning experiment. The cell invasion ability in each group was detected by Transwell method. Results: Compared with the siRNA-control sequence group and the blank control group, the relative expression levels of YAP1 mRNA and protein in siRNA-YAP1 group decreased (P<0.05). Compared with the siRNA-control group and the blank control group, the absorbance A values at 24h, 48h, 72h, and 96h in the siRNA-YAP1 group were decreased (P<0.05). Compared with the siRNA-control sequence group and the blank control group, the apoptosis rate in the siRNA-YAP1 group was increased (P<0.05). Compared with the siRNA-control sequence group and the blank control group, the number of invasive cells in the siRNA-YAP1 group was decreased (P<0.05). Conclusion: Specific silencing of YAP1 gene in human tongue cancer SCC-4 cells could inhibitell proliferation, accelerate cell apoptosis, and reduce cell migration and invasion.

Key words: Tongue squamous cell carcinoma, YAP1, Small interfering RNA, Biological characteristics

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