两种重度牙周炎相关微生物高通量检测

doi:10.13701/j.cnki.kqyxyj.2022.05.006

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (5): 412-416.DOI: 10.13701/j.cnki.kqyxyj.2022.05.006

两种重度牙周炎相关微生物高通量检测

张艺龄, 徐杰^*

昆明医科大学附属口腔医院牙周病科云南昆明 650000

收稿日期:2021-10-25 出版日期:2022-05-28 发布日期:2022-05-20
通讯作者: *徐杰,E-mail:14799731@qq.com
作者简介:张艺龄(1992~ ),女,云南玉溪人,硕士,医师,研究方向:牙周炎与牙周微生物。
基金资助:
云南省科技厅科技计划项目[编号:2018FE001(-261)] 云南省卫生科技计划项目(编号:2017NS267)

High-throughput Detection of Microorganisms in Two Periodontitis

ZHANG Yiling, XU Jie^*

Department of Periodontology, Affiliated Stomatological Hospital, Kunming Medical University, Kunming 650000, China

Received:2021-10-25 Online:2022-05-28 Published:2022-05-20

摘要/Abstract

摘要： 目的: 利用高通量测序(high-throughput sequencing, HTS)技术分析广泛型侵袭性牙周炎(generalized aggressive periodontitis, GAgP)和重度慢性牙周炎(severe chronic periodontitis, SCP)龈下微生物群的组成和差异。方法: 分别选择20例GAgP、22例SCP及20例牙周健康者,采集龈下菌斑,提取DNA,利用Illumina MiSeq测序平台基于16S rDNA的V4可变区检测龈下菌群。结果: (1)优势菌群分析:GAgP组和SCP组的优势菌群结构相似,包括牙髓卟啉单胞菌等;而牙周健康组的优势菌群结构与GAgP组和SCP组不同,包括副流感嗜血杆菌等。(2)LEfSe分析:GAgP组和SCP组之间比较无差异性生物标志物;GAgP组、SCP组分别与牙周健康组比较均存在差异性生物标志物。结论: GAgP和SCP龈下微生物群结构相似,而GAgP和SCP与牙周健康者龈下微生物群结构不同。未发现GAgP和SCP的差异性生物标志物。

关键词: 广泛型侵袭性牙周炎, 重度慢性牙周炎, 龈下菌群, 高通量测序

Abstract: Objective: To analyse the composition and differences of subgingival microorganisms between generalized aggressive periodontitis (GAgP) and severe chronic periodontitis (SCP) by high-throughput sequencing. Methods: Accordingly, 20 patients with GAgP and 22 patients with SCP were enrolled along with 20 healthy subjects. Their subgingival plaques were collected. DNA of the subgingival plaques were extracted. Subgingival bacteria were detected using the illumina Miseq sequencing platform as well as the V4 region of the 16S rDNA of bacteria. Results: The major bacteria of the GAgP and SCP groups were similar, including Porphyromonas endodontalis and so on. Additionally, the dominant bacteria in the healthy group was different from those in the GAgP and SCP groups, including Haemophilus parainfluenzae and so on. No biomarkers were present between the GAgP and SCP groups. Moreover, different biomarkers were compared between the healthy group and the GAgP or the SCP. Conclusion: The GAgP and SCP groups contained similar microbial communities. However, the GAgP or SCP groups as well as the healthy subjects were comprised of different microbial communities. There were no biomarkers between the GAgP and SCP groups.

Key words: generalized aggressive periodontitis, severe chronic periodontitis, subgingival bacteria, high-throughput sequencing

张艺龄, 徐杰. 两种重度牙周炎相关微生物高通量检测[J]. 口腔医学研究, 2022, 38(5): 412-416.

ZHANG Yiling, XU Jie. High-throughput Detection of Microorganisms in Two Periodontitis[J]. Journal of Oral Science Research, 2022, 38(5): 412-416.

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两种重度牙周炎相关微生物高通量检测

High-throughput Detection of Microorganisms in Two Periodontitis

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