口腔医学研究

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (10): 946-949.DOI: 10.13701/j.cnki.kqyxyj.2022.10.009

• 牙体牙髓病学研究 • 上一篇    下一篇

DLX2基因多态性与氟斑牙的相关性分析

昝斌彬1, 陈黎明1,2*   

  1. 1.遵义医科大学 贵州 遵义 563000;
    2.贵阳市口腔医院牙体牙髓病科 贵州 贵阳 550002
  • 收稿日期:2022-02-08 出版日期:2022-10-28 发布日期:2022-10-20
  • 通讯作者: *陈黎明,E-mail:15180711430@163.com
  • 作者简介:昝斌彬(1995~ ),女,四川广元人,硕士在读,主要从事牙体牙髓疾病研究。
  • 基金资助:
    贵州省卫生健康委科学技术基金项目(编号:gzwkj2021-340)

Correlation Analysis of DLX2 Gene Polymorphism and Dental Fluorosis

ZAN Binbin1, CHEN Liming1,2*   

  1. 1. Zunyi Medical University, Zunyi 563000, China;
    2. Department of Endodontics, Guiyang Hospital of Stomatology, Guiyang 550002, China
  • Received:2022-02-08 Online:2022-10-28 Published:2022-10-20

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摘要： 目的: 观察DLX2基因多态性在毕节市燃煤污染型氟斑牙人群中的分布,探讨氟斑牙遗传易感性和耐受性与DLX2基因多态性的关系。方法: 采用病例对照研究,在贵州省毕节市(氟中毒病区)纳入117例氟斑牙患者(实验组)和108例非氟斑牙受试者(内对照组),在贵阳市两城区(非病区)纳入115例非氟斑牙受试者(外对照组)。收集所有受试者血常规检测后剩余的外周血样本,提取血样中的基因组DNA,筛选SNP位点并进行PCR扩增,将扩增产物采用Sanger测序技术对单核苷酸多态性位点基因型进行测定,分析基因型和等位基因频率在各组间的差异。结果: rs743605位点基因型及等位基因频率分布在各组之间无显著性差异(P>0.05),在轻、中、重度氟斑牙各分度之间,CC基因型在重度氟斑牙中的频率明显高于轻度和中度,T等位基因在重度氟斑牙中的频率明显低于轻度和中度,差异有统计学意义(P<0.05)。rs3762499位点基因型分布在各组之间的差异有统计学意义(P<0.05),其中内对照组中的AG基因型频率明显高于实验组,差异有统计学意义(P<0.05);在轻、中、重度氟斑牙各分度之间,此位点基因型及等位基因频率分布无显著性差异(P>0.05)。结论: DLX2基因的rs743605位点和rs3762499位点的基因多态性与氟斑牙存在相关性。

关键词: 同源盒基因, DLX2基因, 氟斑牙, 基因多态性

Abstract: Objective: To observe the distribution of DLX2 gene polymorphism in the population with dental fluorosis caused by coal-burning pollution in Bijie city, and to explore the relationship between genetic susceptibility and tolerance of dental fluorosis and DLX2 gene polymorphism. Methods: Under a case-control study, 117 dental fluorosis patients (experimental group) and 108 non-dental fluorosis subjects (internal control group) were enrolled in Bijie city (fluorosis area), and 115 non-dental fluorosis subjects (external control group) were enrolled in Guiyang two urban areas (non-epidemic area). Their remaining peripheral blood samples after routine blood test were collected. The genomic DNA from blood samples were extracted. SNP loci was screened for PCR amplification, and the amplification products were used Sanger sequencing technology to detect the single nucleotide polymorphism sites' genotypes. The differences of different genotypes and allele frequency distribution among groups were analyzed. Results: There was no significant difference in genotype and allele frequency distribution of rs743605 loci among groups (P>0.05). The frequency of CC genotype was significantly higher in severe dental fluorosis than that in mild and moderate dental fluorosis. The frequency of T allele in severe dental fluorosis was significantly lower than that in mild and moderate dental fluorosis, with difference statistically significant (P<0.05). The distribution of rs3762499 genotype was significantly different among groups (P<0.05). The AG genotype frequency in the internal control group was significantly higher than that in the experimental group (P<0.05). There was no significant difference in genotype and allele frequency distribution among mild, moderate, and severe dental fluorosis (P>0.05). Conclusion: The polymorphism of rs743605 and rs3762499 in DLX2 gene is correlated with dental fluorosis.

Key words: homeobox gene, DLX2 gene, dental fluorosis, gene polymorphism