Fam83h突变对小鼠成骨细胞增殖和迁移的影响

doi:10.13701/j.cnki.kqyxyj.2023.05.011

口腔医学研究 ›› 2023, Vol. 39 ›› Issue (5): 435-439.DOI: 10.13701/j.cnki.kqyxyj.2023.05.011

Fam83h突变对小鼠成骨细胞增殖和迁移的影响

贺桢茹, 郑雪晴, 杨春晖, 汪鑫, 李阳, 宋亚玲^*

口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室,武汉大学口腔医学院湖北武汉 430079

收稿日期:2022-11-09 出版日期:2023-05-28 发布日期:2023-05-16
通讯作者: *宋亚玲,E-mail: sningya@whu.edu.cn
作者简介:贺桢茹(1993~ ),女,重庆荣昌人,硕士在读,研究方向:骨发育。
基金资助:
国家自然科学基金(编号:81670976)

Effects of Fam83h Mutation on Proliferation and Migration of Mouse Osteoblasts

HE Zhenru, ZHENG Xueqing, YANG Chunhui, WANG Xin, LI Yang, SONG Yaling^*

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China

Received:2022-11-09 Online:2023-05-28 Published:2023-05-16

摘要/Abstract

摘要： 目的: 探究Fam83h突变对小鼠成骨细胞(mouse osteoblasts,MOBs)增殖和迁移的影响以及其相关机制。方法: Micro-CT扫描分析5月龄Fam83h突变纯合子小鼠和野生型小鼠的全身骨;体外分离培养Fam83h突变纯合子小鼠和野生型小鼠的MOBs,利用CCK-8实验、划痕实验研究Fam83h突变对MOBs增殖和迁移的影响并测序分析细胞转录组差异基因,Western blot检测相关通路关键分子表达情况。结果: Fam83h突变纯合子小鼠全身骨长及骨体积分数均小于野生型小鼠(P＜0.001)。体外实验结果显示,Fam83h突变纯合子小鼠MOBs的增殖和迁移能力均显著低于野生型小鼠(P＜0.01),KEGG Pathway富集分析提示PI3K/AKT通路差异明显,Western blot结果显示Fam83h突变纯合子小鼠的MOBs的p-AKT1-S473的蛋白水平降低。结论: Fam83h突变可能通过PI3K/AKT通路抑制MOBs的增殖和迁移从而导致小鼠骨量减少。

关键词: Fam83h突变, 成骨细胞, 细胞增殖, 细胞迁移

Abstract: Objective: To explore the effect of Fam83h mutation on proliferation and migration of mouse osteoblasts (MOB) and the related mechanism. Methods: The whole body of wild-type and Fam83h-mutated mice were analyzed by micro-CT. CCK-8 and wound healing were detected in MOBs isolated from 3-day-old wild-type and Fam83h-mutated mice. RNA-seq was performed to compare the transcriptional profile of MOBs of Fam83h-mutated mice with wild-type mice. Phosphorylation of AKT1-S473 and AKT1 were measured by Western blot. Results: Fam83h-mutated mice showed decreased bone volume fraction (P＜0.001). Fam83h mutation significantly repressed cell proliferation and migration in MOBs (P<0.01). KEGG pathway analysis of differentially expressed genes (DEGs) identified PI3K/AKT signaling pathway. Furthermore, the protein expression level of p-AKT1-S473 was decreased in MOBs of Fam83h mutation mice. Conclusion: Fam83h mutation may inhibit the proliferation and migration of MOBs through PI3K/AKT signaling pathway, resulting in bone mass loss in Fam83h-mutated mice

Key words: Fam83h mutation, osteoblast, cell proliferation, cell migration

贺桢茹, 郑雪晴, 杨春晖, 汪鑫, 李阳, 宋亚玲. Fam83h突变对小鼠成骨细胞增殖和迁移的影响[J]. 口腔医学研究, 2023, 39(5): 435-439.

HE Zhenru, ZHENG Xueqing, YANG Chunhui, WANG Xin, LI Yang, SONG Yaling. Effects of Fam83h Mutation on Proliferation and Migration of Mouse Osteoblasts[J]. Journal of Oral Science Research, 2023, 39(5): 435-439.

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Fam83h突变对小鼠成骨细胞增殖和迁移的影响

Effects of Fam83h Mutation on Proliferation and Migration of Mouse Osteoblasts

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