低龄儿童龋患者唾液微生物群落研究及预测模型构建

doi:10.13701/j.cnki.kqyxyj.2024.09.005

口腔医学研究 ›› 2024, Vol. 40 ›› Issue (9): 778-784.DOI: 10.13701/j.cnki.kqyxyj.2024.09.005

低龄儿童龋患者唾液微生物群落研究及预测模型构建

林秀燕, 赵彩云, 石宏^*

河北医科大学口腔医学院•口腔医院儿童口腔科,河北省口腔医学重点实验室, 河北省口腔疾病临床医学研究中心河北石家庄 050011

收稿日期:2024-03-08 出版日期:2024-09-28 发布日期:2024-09-25
通讯作者: ^*石宏,E-mail:shihong_hb@163.com
作者简介:林秀燕(1996~),女,福建连江人,医师,硕士,主要从事儿童口腔医学研究。

Study on Saliva Microbial Community and Construction of Prediction Model for Young Children with Caries

LIN Xiuyan, ZHAO Caiyun, SHI Hong^*

School of Stomatology, Hebei Medical University, Department of Pediatric Dentistry, Hebei Provincial Key Laboratory of Stomatology, Hebei Provincial Clinical Research Center for Oral Diseases, Shijiazhuang 050011, China

Received:2024-03-08 Online:2024-09-28 Published:2024-09-25

摘要/Abstract

摘要： 目的:对不同患龋儿童唾液进行高通量测序并分析低龄儿童龋(early childhood caries,ECC)的影响因素,为探究ECC与菌群的相关性及ECC综合诊断模型的建立提供依据。方法:对儿童口腔检查,根据结果分为无龋(caries free,CF)组、ECC组和重度低龄儿童龋(severe early childhood caries,SECC)组,采取问卷调查方法探究ECC的影响因素;同时对儿童唾液进行高通量测序,并拟采用受试者工作曲线(receiver operating characteristic curve,ROC)建立基于以上结果的疾病诊断模型。结果:不同患龋儿童的微生物群落组成存在显著统计学差异(P＜0.05)。在前15个细菌属中,韦荣菌属(Veillonella)、纤毛菌属(Leptotrichia)、拟普雷沃菌属(Alloprevotella)丰度水平的差异在3组间具有统计学意义(P＜0.05)。Veillonella和Leptotrichia为ECC组的标志物种,Alloprevotella在SECC组中显著富集。戒断奶瓶喂养的年龄、Veillonella、Leptotrichia、Alloprevotella联合应用筛选高患龋风险儿童的ROC曲线下面积为0.754。结论:Veillonella、Leptotrichia、Alloprevotella可能为患龋儿童唾液中潜在的致龋菌。戒断奶瓶喂养的年龄、Veillonella、Leptotrichia、Alloprevotella联合检测对筛选高患龋儿童具有诊断价值。

关键词: 低龄儿童龋, 微生物群落, 预测模型, 高通量测序

Abstract: Objective: To analyze the influencing factors of early childhood caries (ECC) and to provide a basis for exploring the correlation between ECC and flora and establishing a comprehensive diagnostic model of ECC through high-throughput sequencing on saliva of children with different caries status. Methods: Children were examined orally and divided into three groups according to the Results: Caries free (CF) group, ECC group, and Severe early childhood caries (SECC) group. The influencing factors of ECC were investigated by questionnaire. At the same time, saliva of three groups of children was collected for high-throughput sequencing, and a disease diagnosis model based on the above results was established by receiver operating characteristic curve (ROC). Results: There was significant statistical difference in the composition of microbial communities among groups with different caries status (P<0.05). Among the top 15 bacterial genera, the abundance levels of Veillonella, Leptotrichia, and Alloprevotella were significantly different (P<0.05). Veillonella and Leptotrichia were the marker species of ECC group. Alloprevotella was significantly enriched in SECC group. The area under the ROC curve for screening children with high caries risk was 0.754, which was based on the age of weaning from bottle feeding, the combined application of Veillonella, Leptotrichia, and Alloprevotella. Conclusion: Veillonella, Leptotrichia, and Alloprevotella may be potential cariogenic bacteria in saliva of children with caries. The age of weaning from bottle feeding, the combined application of Veillonella, Leptotrichia, and Alloprevotella are of diagnostic value for screening children with high caries.

Key words: early childhood caries, microbial community, prediction model, high-throughput sequencing

林秀燕, 赵彩云, 石宏. 低龄儿童龋患者唾液微生物群落研究及预测模型构建[J]. 口腔医学研究, 2024, 40(9): 778-784.

LIN Xiuyan, ZHAO Caiyun, SHI Hong. Study on Saliva Microbial Community and Construction of Prediction Model for Young Children with Caries[J]. Journal of Oral Science Research, 2024, 40(9): 778-784.

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低龄儿童龋患者唾液微生物群落研究及预测模型构建

Study on Saliva Microbial Community and Construction of Prediction Model for Young Children with Caries

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