OSAHS儿童与非OSAHS儿童唾液差异蛋白质组学研究

doi:10.13701/j.cnki.kqyxyj.2025.04.007

摘要/Abstract

摘要： 目的: 通过蛋白质组学检测技术分析阻塞性睡眠呼吸暂停低通气综合征(obstructive sleep apnea hypopnea syndrome, OSAHS)儿童和非OSAHS儿童唾液差异蛋白的相关信息,为深入研究OSAHS发病机制,探寻OSAHS生物标志物及关键治疗靶点提供研究基础。方法: 收集6例OSAHS儿童[男5例、女1例,平均年龄(12.00±2.71)岁]及6例非OSAHS健康儿童[男3例、女3例,平均年龄(10.22±2.05)岁]唾液样本,进行总蛋白质提取,采用数据非依赖采集(data-independent acquisition,DIA)质谱分析和对差异蛋白行蛋白质鉴定,最后对被筛选的差异蛋白进行生物信息学分析。结果: 非OSAHS组中有5636个蛋白、OSAHS组中有5608个蛋白被鉴定,其中5582个蛋白在所有组中都存在。筛选出两组之间的显著差异蛋白,303个蛋白上调表达和185个蛋白下调表达,其中筛选了KRT80、CXCL10、KRT19、SAA2等差异显著的蛋白。结论: OSAHS儿童和非OSAHS儿童唾液蛋白表达存在差异,相关分子标志物可能与OSAHS发生、发展、发病机制密切相关。

关键词: 阻塞性睡眠呼吸暂停低通气综合征, 唾液蛋白, 蛋白质组学, 生物信息学分析, DIA技术, 差异蛋白, 整夜多导睡眠监测, 张口呼吸

Abstract: Objective: To analyze the related information of salivary differential proteins between OSAHS children and non-OSAHS children through proteomics detection technology. Methods: Saliva samples were collected from 6 children with OSAHS [5 males and 1 female, with an average age of (12.00±2.71) years] and 6 non-OSAHS healthy children [3 males and 3 females, with an average age of (10.22±2.05) years]. Total proteins were extracted, data-independent acquisition (DIA) mass spectrometry analysis and protein identification of differential proteins were performed, and finally bioinformatics analysis was conducted on the screened differential proteins. Results: 5636 proteins were identified in the non-OSAHS group and 5608 proteins in the OSAHS group, among which 5582 proteins were present in all groups. A total of 303 up-regulated proteins and 185 down-regulated proteins were screened out as significant differences between the two groups, among which several proteins with significant differences, such as KRT80, CXCL10, KRT19, and SAA2, were selected. Conclusion: There are differences in salivary proteins between OSAHS children and non-OSAHS children, and OSAHS molecular markers may be closely related to its occurrence, development, and pathogenesis.

Key words: obstructive sleep apnea hypopnea syndrome, salivary protein, proteomics, bioinformaticsanalysis , data-independent acquisition technology, differential protein, polysomnography, mouth breathing

祖丽胡马尔·努尔艾合买提, 麦克丽亚·帕力哈提, 古力巴哈·买买提力. OSAHS儿童与非OSAHS儿童唾液差异蛋白质组学研究[J]. 口腔医学研究, 2025, 41(4): 306-313.

Zulihumaer·NUERAIHEMAITI, Maikeliya·PALIHATI, Gulibaha·MAIMAITILI. Proteomic Study on Salivary Differences between OSAHS Children and Non-OSAHS Children[J]. Journal of Oral Science Research, 2025, 41(4): 306-313.

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