口腔医学研究

口腔医学研究 ›› 2026, Vol. 42 ›› Issue (1): 48-52.DOI: 10.13701/j.cnki.kqyxyj.2026.01.009

• 口腔黏膜病学研究 • 上一篇    下一篇

荧光染色直接镜检法在口腔念珠菌微生物学诊断中的应用

单婧1,2,3, 范媛1,2,3, 许隽永1,2,3, 刘青兰1,2,3, 钱棱1,2,3*   

  1. 1.南京医科大学附属口腔医院口腔黏膜病科 江苏 南京 210029;
    2.口腔疾病研究与防治国家级重点实验室培育建设点 江苏 南京 210029;
    3.江苏省口腔转化医学工程研究中心 江苏 南京 210029
  • 收稿日期:2025-02-27 出版日期:2026-01-28 发布日期:2026-01-23
  • 通讯作者: *钱棱,E-mail:qlqianleng@163.com
  • 作者简介:单婧(1989~ ),女,江苏盐城人,博士,主治医师,研究方向:口腔黏膜病学。
  • 基金资助:
    江苏省大学生创新创业训练计划项目(编号:202410312111Y);江苏省科教能力提升工程-江苏省研究型医院建设单位(编号:YJXYYJSDW4);江苏省医学创新中心(编号:CXZX202227)

Application of Fluorescence Staining Direct Microscopy Method in Microbiological Diagnosis of Oral Candidiasis

SHAN Jing1,2,3, FAN Yuan1,2,3, XU Juanyong1,2,3, LIU Qinglan1,2,3, QIAN Ling1,2,3*   

  1. 1. Department of Oral Mucosal Diseases, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing 210029, China;
    2. State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing 210029, China;
    3. Jiangsu Province Engineering Research Center of Stomatological Translational, Nanjing 210029, China
  • Received:2025-02-27 Online:2026-01-28 Published:2026-01-23

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摘要： 目的: 明确荧光染色直接镜检法在口腔念珠菌感染中的应用价值。方法: 纳入263例怀疑口腔念珠菌感染的患者进行真菌培养、荧光染色法直接镜检和氢氧化钾(potassium hydroxide,KOH)镜检。结果: 263例样本中,真菌荧光染色法直接镜检的灵敏度为61.73%,特异度为96.15%,阳性预测值为87.72%,阴性预测值为84.95%,而KOH镜检法的灵敏度为16.67%,特异度为96.32%,阳性预测值为66.67%,阴性预测值为72.35%。使用真菌荧光染色法对口腔扁平苔藓和口腔念珠菌病患者进行检查,发现其灵敏度分别为35.29%和88.46%;而KOH法的灵敏度仅分别为5.88%和35%。结论: 荧光染色直接镜检法灵敏度较高,操作简单、结果迅速,可为临床的诊治工作提供有效的实验室支持。

关键词: 荧光染色法, 真菌检查, 口腔念珠菌

Abstract: Objective: To clarify the application value of direct microscopy using fluorescence staining method in oral candidal infection. Methods: Two hundred and sixty-three patients suspected of oral candidiasis were included for fungal culture, fluorescence staining direct microscopy, and potassium hydroxide (KOH) microscopy examination. Results: The sensitivity, specificity, the positive predictive value, and the negative predictive value of direct microscopy using fungal fluorescence staining of 263 samples were 61.73%, 96.15%, 87.72%, and 84.95%, respectively. While the sensitivity, specificity, the positive predictive value, and the negative predictive value of KOH microscopy were 16.67%, 96.32%, 66.67%, and 72.35%, respectively. Using fungal fluorescence staining method to examine patients with oral lichen planus and oral candidiasis, the sensitivity was found to be 35.29% and 88.46%, respectively. The sensitivity of KOH method was only 5.88% and 35%, respectively. Conclusion: The fluorescence staining direct microscopy method had high sensitivity, simple operation, and rapid results, which could provide effective laboratory support for clinical diagnosis and treatment.

Key words: fluorescence staining method, fungal examination, oral candidiasis