活化卫星胶质细胞参与牙髓炎症及痛觉过敏的实验研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (2): 117-119.

活化卫星胶质细胞参与牙髓炎症及痛觉过敏的实验研究

王海棠,王云,田英,阳司敏,张丽丽,杨亚黎,金程,金海威^*

大连医科大学口腔医学院辽宁大连 116044

收稿日期:2014-07-22 出版日期:2015-02-28 发布日期:2016-04-29
通讯作者: 金海威,E-mail:haiwei.jin@dlmedu.edu.cn
作者简介:王海棠(1988~ ),陕西人,硕士在读,主要从事口腔颌面部疼痛的医学研究。
基金资助:
辽宁省自然科学基金项目(2013023051) 教育部留学回国人员科研启动基金资助项目

Experimental Study on the Activated SGCs Involved in the Pulpitis and Hyperalgesia in Rats.

WANG Hai-tang, WANG Yun, TIAN Ying, et al

College of Stomatology, Dalian Medical University, Dalian 116044

Received:2014-07-22 Online:2015-02-28 Published:2016-04-29

摘要/Abstract

摘要： 目的:观察大鼠实验性牙髓炎过程中三叉神经节GFAP的表达变化,探讨三叉神经系统通过卫星胶质细胞活化反应参与牙髓炎症及痛觉过敏的潜在机制。方法:建立大鼠牙髓炎动物模型,应用免疫荧光染色及RT-PCR半定量分析方法,从细胞水平及mRNA水平检测三叉神经节中GFAP的动态表达。结果:大鼠牙髓炎症24 h后,三叉神经节内可见GFAP免疫阳性卫星胶质细胞围绕在神经元周围,形成典型的环状结构。RT-PCR半定量分析结果显示,GFAP的mRNA表达水平于炎症24 h达峰值。结论:三叉神经系统通过卫星胶质细胞活化反应参与牙髓炎症及痛觉过敏的发生和发展。

关键词: 牙髓炎, GFAP, 卫星胶质细胞, 痛觉过敏

Abstract: Objective: To investigate the dynamic expression of GFAP in the trigeminal ganglion (TG), and to elucidate the potential role of activated SGCs in the trigeminal nervous system that involved in the development of pulpitis and associated hyperalgesia. Methods: A rat animal-model with pulpitis was induced by performing CFA on Sprague-Dawley rats. The dynamic expression of GFAP in TG was demonstrated by immunofluorescence, and the mRNA of GFAP was detected by semi-quantitative reverse transcription PCR. Results: The GFAP-positive cells were found to present a typical morphology of circle that surrounded the neurons in TG after 24 hours post CFA-inflammation. Results of semi-quantitative RT-PCR showed that the mRNA of GFAP was increased significantly and reached the peak at 24 hours post CFA-inflammation compared to the control. Conclusion: The trigeminal nervous system may participate in the histopathological development of pulpitis and hyperalgesia with the activation of SGCs.

Key words: Pulpitis , GFAP, SGCs, Hyperalgesia

中图分类号:

R780.1

王海棠,王云,田英,阳司敏,张丽丽,杨亚黎,金程,金海威. 活化卫星胶质细胞参与牙髓炎症及痛觉过敏的实验研究[J]. 口腔医学研究, 2015, 31(2): 117-119.

WANG Hai-tang, WANG Yun, TIAN Ying, et al. Experimental Study on the Activated SGCs Involved in the Pulpitis and Hyperalgesia in Rats.[J]. Journal of Oral Science Research, 2015, 31(2): 117-119.

[1]	陈杰,徐华兴,程抒华,张旗. 细胞自噬在牙髓损伤修复过程中的作用[J]. 口腔医学研究, 2019, 35(6): 531-536.
[2]	丛芳, 倪慧珍, 李维佳, 樊帆, 金海威. CD16参与大鼠外周神经损伤及痛觉过敏的研究[J]. 口腔医学研究, 2017, 33(6): 605-608.
[3]	何琼琼,杨颖,黄声富,樊明文. TLR4,NOD2和NLRP3在牙髓炎中的表达[J]. 口腔医学研究, 2017, 33(5): 496-499.
[4]	王素,李进红,毕成,周毅. 不同剂量阿替卡因对不可复性牙髓炎下牙槽神经阻滞麻醉效果的比较[J]. 口腔医学研究, 2016, 32(8): 874-876.
[5]	张丽丽, 阳司敏, 丛芳, 倪慧珍, 李维佳, 金海威. CX3CL1与CX3CR1参与牙髓炎症及痛觉过敏的实验研究[J]. 口腔医学研究, 2016, 32(7): 685-688.
[6]	梅雪,谢俐萍,金幼虹,叶芳,熊伟,岳黎,姜珊. iRoot BP Plus直接盖髓后比格犬牙髓的反应性变化[J]. 口腔医学研究, 2016, 32(12): 1234-1239.
[7]	陈霞, 袁通穗, 叶芳, 孙瑜, 杨健, 郭俊, 金幼虹. 牙髓炎不同阶段P2X受体家族在大鼠脑干中的表达变化研究[J]. 口腔医学研究, 2016, 32(10): 1033-1037.
[8]	刘杨,苗莉,赵雅娟,王舰,张旻,赵蕊妮,陈永进,赵文峰. 卫星胶质细胞活化在束缚应激致大鼠咬肌痛觉敏感中的作用[J]. 口腔医学研究, 2015, 31(12): 1174-1178.