口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (7): 685-688.DOI: 10.13701/j.cnki.kqyxyj.2016.07.005

• 基础研究论著 • 上一篇    下一篇

CX3CL1与CX3CR1参与牙髓炎症及痛觉过敏的实验研究

张丽丽, 阳司敏, 丛芳, 倪慧珍, 李维佳, 金海威*   

  1. 大连医科大学口腔医学院颌面外科 辽宁 大连 116044
  • 收稿日期:2016-01-18 出版日期:2016-07-26 发布日期:2016-07-25
  • 通讯作者: 金海威,E-mail:haiwei.jin@dlmedu.edu.cn
  • 作者简介:张丽丽(1987~ ),女,河北人,硕士在读,主要从事口腔颌面部疼痛的研究。
  • 基金资助:
    辽宁省自然科学基金项目(2013023051);教育部留学回国人员科研启动基金资助项目

Involvement of CX3CL1 and CX3CR1 in Pulpitis and Hyperalgesiaof Rats.

ZHANG Li-li, YANG Si-min, CONG Fang, NIHui-Zhen, Li Wei-Jia, JIN Hai-Wei*.   

  1. School of Stomatology, Dalian Medical University, Dalian 116044, China
  • Received:2016-01-18 Online:2016-07-26 Published:2016-07-25

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摘要： 目的: 观察大鼠实验性牙髓炎过程三叉神经节中CX3CL1与CX3CR1的动态表达,探讨三叉神经系统参与牙髓炎病理改变的信号调节作用。方法: 建立大鼠牙髓炎动物模型,应用免疫荧光染色方法检测三叉神经节中CX3CL1与CX3CR1的表达变化。结果: 免疫荧光染色结果显示,CX3CL1免疫阳性卫星胶质细胞围绕在神经元周围,形成典型的环状结构,而CX3CR1主要表达在神经元细胞体上。统计学分析显示, CFA致炎后2周,CX3CL1阳性细胞率达到峰值,明显高于正常对照组;CFA致炎后24 h及72 h,CX3CR1阳性细胞率与正常对照组相比明显增高。结论: 三叉神经系统通过调节CX3CL1与CX3CR1的表达参与牙髓炎症及痛觉过敏的发生发展。

关键词: 牙髓炎, 痛觉过敏, 三叉神经节, CX3CL1, CX3CR1

Abstract: Objective: To investigate thedynamic expressionofCX3CL1 and CX3CR1 in the trigeminal ganglions (TGs) and furthermore toelucidate the potential roles ofCX3CL1 and CX3CR1 in the trigeminal nervous system that involved in the development of the inflammatory response and hyperalgesia induced by pulpitis. Methods: An animal-model of pulpitiswas established. The dynamic expression ofCX3CL1 and CX3CR1 inTGswasobserved byimmunofluorescencetechnique. Results: CX3CL1and CX3CR1-positive cells were found in boththe experimental and control TGs by immunofluorescentstaining. The expression of CX3CL1profiles were presented typical morphology of circles surroundingthe neurons, while CX3CR1wereexpressed inTG neurons. Statistical analysis revealed that the numbers ofCX3CL1and CX3CR1-positive cells were increased induced byCFA-inflammation. The rate of CX3CL1-positive cellsreached the peak at 2weeksand decreased to normal level at 6 weeks. The rate of CX3CR1-positive cells was significantly higherthanthat ofthe control group at 24 hours and 72 hoursafter CFA-inflammation. Conclusion: The expression of CX3CL1 and CX3CR1 may participate in the inflammatory response of pulpitis and hyperalgesia intrigeminal nervous system.

Key words: Pulpitis, Hyperalgesia, Trigeminal ganglion, CX3CL1 , CX3CR1

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