大蒜素标准品DATS体外抗粪肠球菌生物膜作用的研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (9): 867-870.

大蒜素标准品DATS体外抗粪肠球菌生物膜作用的研究

王祥柱¹,谢晓莉^1*,陈敏慜¹,冯海燕²

1. 中南大学湘雅口腔医院牙体牙髓科湖南长沙 410078;
2. 长沙市口腔医院牙体牙髓科

收稿日期:2015-01-20 出版日期:2015-09-28 发布日期:2016-05-04
通讯作者: 谢晓莉,E-mail:xiexli@msn.com
作者简介:王祥柱(1986~ ),男,湖南邵阳人,硕士,医师,主要从事牙体牙髓病的临床治疗工作。
基金资助:
湖南省科技厅科研基金资助项目(2013SK3071);湖南省卫生厅科研基金资助项目(B2014-011)

Effects of Standard Allicin on Enterococcus Faecalis Biofilms Control: an in vitro Study

WANG Xiang-zhu¹, XIE Xiao-li¹, CHEN Min-min¹, FENG Hai-yan²

1. Department of Endodontics, Xiangya Stomatological Hospital, Central South University, Changsha 410078, China;
2. Department of Endodontics, Changsha Stomatological Hospital, Changsha 410000, China

Received:2015-01-20 Online:2015-09-28 Published:2016-05-04

摘要/Abstract

摘要： 目的:探讨不同浓度大蒜素对照品二烯丙基化三硫(dially trisulfide,DATS)体外对粪肠球菌生物膜的作用。方法:体外培养形成粪肠球菌生物膜,分别经1280、2560、5120 mg/L浓度的DATS作用后,采用MTT法观察DATS对生物膜粪肠球菌的抑菌率。结果:1280 mg/L DATS对对数期和饥饿期的生物膜粪肠球菌抑菌率约71%,显著高于对稳定期生物膜粪肠球菌的抑菌率58%,差异具有统计学意义(P<0.05),随着药物浓度的增高,抑菌率增高。结论:DATS对生物膜中的粪肠球菌具有明显杀灭或抑制作用。

关键词: 大蒜素, 粪肠球菌, 细菌生物膜

Abstract: Objective: To investigate the antibacterial efficacy of different concentrations dially trisulfide (DATS) on the biofilms of Enterococcus faecalis (E. faecalis) in vitro. Methods: E. faecalis was cultured in vitro to form the bacterial biofilm of different growth periods. Different concentrations of DATS, i.e. 1280μg/mL, 2560μg/mL and 5120μg/mL, were added to E. faecalis biofilms in different growth periods, and the bacteriostasis rates were measured by MTT method. Results: The bacteriostatic rates of 1280μg/mL DATS were about 71% in logarithmic phase and hunger phase, which were significantly higher than that in stability phase with bacteriostasis rate of 58% (P<0.05). DATS in concentration of 2560μg/mL and 5120μg/mL showed better antibacterial efficacy than that of 1280μg/mL, indicating that the antibacterial efficacy of DATS was in a concentration-dependent manner (P<0.05). Conclusion: DATS played a role in suppressing E. faecalis in the biofilm in vitro.

Key words: Allic, Enterococcus faecalis, Bacterial biofilm

中图分类号:

R780.1

王祥柱,谢晓莉,陈敏慜,冯海燕. 大蒜素标准品DATS体外抗粪肠球菌生物膜作用的研究[J]. 口腔医学研究, 2015, 31(9): 867-870.

WANG Xiang-zhu, XIE Xiao-li, CHEN Min-min, FENG Hai-yan. Effects of Standard Allicin on Enterococcus Faecalis Biofilms Control: an in vitro Study[J]. Journal of Oral Science Research, 2015, 31(9): 867-870.

参考文献

[1] 林青,乔竟原.大蒜素的药理与临床应用[J].首都医药,2004,6∶38- 39
[2] 孙奋治.大蒜成分及其加工转化产物的命名应统一[J].国药学杂志,1998,33(3)∶170-171
[3] Chavan SD, Shetty NL, Kanuri M. Comparative evaluation of garlic extract mouthwash and chlorhexidine mouthwash on salivary Streptococcus mutans count-an in vitro study [J]. Oral Health Prev Dent, 2010, 8(4)∶369-374
[4] Fujisawa H, Watanabe K, Suma K, et al. Antibacterial potential of garlic-derived allicin and its cancellation by sulfhydryl compounds [J]. Biosci Biotechnol Biochem, 2009,73(9)∶1948-1955
[5] Michael G, Johnson, Reese H. Death of Salmonella typhimurium and Escherichia coli in the Presence of Freshly Reconstituted Dehydrated Garlic and Onion [J]. Appl Microbiol,1969,17(6)∶903-905
[6] Siqueira JF Jr, Ras IN. Clinical implications and microbiology of bacterial persistence after treatment procedures [J]. J Endod, 2008, 34(11)∶1291-1301
[7] Portenier I, Haapasalo H, Orstavik D,et al.Inactivation of the antibacterial activity of iodine potassium iodide and chlorhexidine digluconate against Enterococcus faecalis by dentin, dentin matrix, type-I collagen, and heat-killed microbial whole cells [J]. J Endod, 2002, 28(9)∶634-637
[8] Evans M, Davies JK, Sundqvist G, et al. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide [J]. Int Endod J, 2002, 35(3)∶221-228
[9] Kandaswamy D, Venkateshbabu N, Gogulnath D,et al.Dentinal tubule disinfection with 2% chlorhexidine gel, propolis, morinda citrifolia juice, 2% povidone iodine, and calcium hydroxide [J]. Int Endod J, 2010, 43(5)∶419-423
[10] 谢晓莉,尹晓敏,刘虹.大蒜素对感染根管渗出液IL-6影响的初步研究[J].牙体牙髓牙周病学杂志,2007,17(2)∶90-92
[11] Newall CA, Anderson LA, Phillipson JD. Herbal medicines: A guide for health-care professionals [J]. London: Pharmaceutical Press,1996,296
[12] Ruddock PS, Liao M, Foster BC, et al. Garlic natural health products exhibit variable constituent levels and antimicrobial activity against Neisseria gonorrhoeae, Staphylococcus aureus and Enterococcus faecalis [J]. Phytother Res, 2005, 19(4)∶327-334
[13] Block E. The chemistry of garlic and onions [J]. Sci Am,1985,252(3)∶114-119
[14] Eswar K,Venkateshbabu N,Rajeswari K. Dentinal tubule disinfection with 2% chlorhexidine, garlic extract, and calcium hydroxide against Enterococcus faecalis by using real-time polymerase chain reaction: In vitro study [J]. J Conserv Dent, 2013, 16(3)∶194-198
[15] Torabinejad M, Shabahang S, Aprecio RM, et al. The antimicrobial effect of MTAD: An in vitro investigation [J]. J Endod, 2003, 29(6)∶400-403[16] Shabahang S, Torabinejad M. Effect of MTAD on enterococcus faecalis-contaminated root canals of extracted human teeth [J]. J Endod, 2003, 29(9)∶576-579
[17] Newberry BM, Shabahang S, Johnson N, et al. The antimicrobial effect of Biopure MTAD on eight strains of enterococcus faecalis: an in vitro investigation [J]. J Endod, 2007, 33(11)∶1352-1354
[18] Davis JM, Maki J, Bahcall JK, et al. An in vitro comparison of the antimicrobial effects of various endodontic medicaments on enterococcus faecalis [J]. J Endod, 2007, 33(5)∶567-569
[19] Krause TA, Liewehr FR, Hahn CI. The antimicrobial effect of MTAD, sodium hypochlorite, doxycycline, and citric acid on Enterococcus faecalis [J]. J Endod, 2007, 33(1)∶28-30
[20] Carson KR, Goodell GG, McClanahan SB. Comparison of the antimicrobial activity of six irrigants on primary endodontic pathogens [J]. J Endod, 2005, 31(6)∶471-473
[21] Mohammadi Z, Mombeinipour A, Giardino L, et al. Residual antibacterial activity of a new modified sodium hypochlorite-based endodontic irrigation solution [J]. Med Oral Patol Oral Cir Bucal, 2011, 16(4)∶588-592
[22] Ardizzoni A, Blasi E, Rimoldi C, et al. An in vitro and ex vivo study on two antibiotic-based endodontic irrigants: A challenge to sodium hypochlorite [J]. New Microbiol, 2009, 32(1)∶57-66