乳酸链球菌素对粪肠球菌生物膜的清除作用

doi:10.13701/j.cnki.kqyxyj.2020.07.007

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (7): 635-638.DOI: 10.13701/j.cnki.kqyxyj.2020.07.007

乳酸链球菌素对粪肠球菌生物膜的清除作用

张旭阳¹, 李慧¹, 刘漪¹, 娄亭亭², 鲍雪滢³, 李诗雅³, 黄辉^1*

1.南昌大学第一附属医院口腔科江西南昌 330006;
2.山东省立医院口腔科山东济南 250000;
3.南昌大学研究生院医学部江西南昌 330006

收稿日期:2019-10-31 出版日期:2020-07-28 发布日期:2020-07-24
通讯作者: 黄辉,E-mail: huanghui1965@126.com
作者简介:张旭阳(1992~),女,湖北钟祥人,医师,硕士,主要从事口腔内科的基础与临床研究工作。

Scavenging Effects of Nisin on Enterococcus faecalis Biofilm

ZHANG Xuyang¹, LI Hui¹, LIU Yi¹, LOU Tingting², BAO Xueying³, LI Shiya³, HUANG Hui^1*

1. Department of Stomatology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China;
2. Department of Stomatology, Shandong Provincial Hospital, Jinan 250000, China;
3. Nanchang University Graduate School of Medicine, Nanchang 330006, China

Received:2019-10-31 Online:2020-07-28 Published:2020-07-24

摘要/Abstract

摘要： 目的: 探讨乳酸链球菌素(Nisin)对粪肠球菌生物膜的体外清除作用。方法: 建立24 h粪肠球菌生物膜的体外模型后,运用MTT法检测各组药物(A1、B1、C1、D1、E1)对粪肠球菌生物膜的清除作用;再用激光共聚焦扫描显微镜(CLSM)观察各组药物(A2、B2、C2、D2、E2)对粪肠球菌生物膜结构的影响。结果: 不同浓度Nisin对粪肠球菌生物膜有一定清除作用(P<0.05),但弱于与阳性对照组(P<0.05),不同浓度对生物膜的清除作用各不相同,除C1组与D1组相比无统计学差异(P>0.05),其余各组相互比较差异均有统计学意义(P<0.05);CLSM观察到,Nisin作用后,生物膜结构逐渐变稀疏,破坏效果显著。结论: Nisin对粪肠球菌生物膜有一定清除和破坏作用。

关键词: 乳酸链球菌素, 粪肠球菌, 生物膜

Abstract: Objective: To explore the scavenging effect of different concentrations of Nisin on Enterococcus faecalis biofilm. Methods: A 24h-old biofilm model of Enterococcus faecalis was established in vitro. The MTT method was applied to evaluate the scavenging effect of Nisin (A1, B1, C1, D1, E1) on the 24h-old Enterococcus faecalis biofilm. The confocal laser scanning microscopy was used to observe the effect of the tested drugs (A2, B2, C2, D2, E2) on the structures of Enterococcus faecalis biofilms in vitro. Results: Nisin of different concentrations scavenged the biofilms to some extent (P<0.05), yet with higher biofilm biomass than that of PC1 (P<0.05). There were different statistical differences when the experimental groups compared within them. There were statistically significant differences between any two in the experimental groups (P<0.05), except for between C1 and D1 (P>0.05). The images of CLSM showed Nisin could damage the biofilm significantly, leaving the biofilm structure becoming sparse. Conclusion: Nisin could scavenge and disrupt the E.faecalis biofilm to some extent.

Key words: Nisin, Enterococcus faecalis, biofilm

张旭阳, 李慧, 刘漪, 娄亭亭, 鲍雪滢, 李诗雅, 黄辉. 乳酸链球菌素对粪肠球菌生物膜的清除作用[J]. 口腔医学研究, 2020, 36(7): 635-638.

ZHANG Xuyang, LI Hui, LIU Yi, LOU Tingting, BAO Xueying, LI Shiya, HUANG Hui. Scavenging Effects of Nisin on Enterococcus faecalis Biofilm[J]. Journal of Oral Science Research, 2020, 36(7): 635-638.

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乳酸链球菌素对粪肠球菌生物膜的清除作用

Scavenging Effects of Nisin on Enterococcus faecalis Biofilm

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