粪肠球菌LTA通过促进ROS的高表达活化NLRP3炎性体

doi:10.13701/j.cnki.kqyxyj.2018.02.002

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (2): 112-116.DOI: 10.13701/j.cnki.kqyxyj.2018.02.002

粪肠球菌LTA通过促进ROS的高表达活化NLRP3炎性体

王丽娜, 叶丹丹, 王娇娇, 牛卫东^*

大连医科大学口腔医学院牙体牙髓科辽宁大连 116044;

收稿日期:2017-10-12 出版日期:2018-02-28 发布日期:2018-02-26
通讯作者: 牛卫东,E-mail:13840886663@139.com
作者简介:王丽娜(1981~ ),女,辽宁锦州人,博士,讲师,主要从事牙体牙髓病学病因学与治疗学研究。
基金资助:
国家自然科学基金(81171538)

Enterococcus Faecalis LTA Activates NLRP3 Inflammasome by Promoting High Expression of ROS

WANG Li-na, YE Dan-dan, WANG Jiao-jiao, NIU Wei-dong^*

Department of Endodontics, School of Stomatology, Dalian Medical University, Dalian 116044, China.

Received:2017-10-12 Online:2018-02-28 Published:2018-02-26

摘要/Abstract

摘要： 目的:检测粪肠球菌脂磷壁酸LTA能否通过激活活性氧而活化NLRP3炎性体。方法:粪肠球菌LTA作用于小鼠巨噬细胞RAW264.7,运用Western blot、ELISA及 Real-time qPCR法检测NLRP3炎性体蛋白及mRNA的表达。还原型烟酰胺腺嘌呤二核苷酸磷酸酶氧化酶抑制剂DPI抑制活性氧(reactive oxygen species,ROS)的产生,荧光染色及流式细胞仪检测ROS的表达。结果:LTA作用于RAW264.7细胞24小时后,NLRP3、Caspase-1、IL-1β蛋白及mRNA的表达量均明显高于阴性对照组(P<0.05)。DPI可有效抑制ROS的产生,ROS表达被抑制后,NLRP3炎性体蛋白及mRNA的表达亦明显降低。结论:LTA是粪肠球菌活化NLRP3炎性体的毒力因子,主要借助于ROS的产生。

关键词: NLRP3炎性体, 粪肠球菌, LTA, ROS

Abstract: Objective: To detect whetherEnterococcus faecalis LTA activates NLRP3 inflammasome by promotingreactive oxygen species expression. Methods: Mouse macrophages RAW264.7 cells were stimulated with Enterococcus faecalis LTA, and the expressions of NLRP3 protein and mRNA were detected by Western blot, ELISA, and real-time qPCR. Reduced nicotinamide adenine dinucleotide phosphatase oxidase inhibitor DPI inhibited the production of reactive oxygen species (ROS), which was detected by fluorescence staining and flow cytometry. Results: After LTA was applied to RAW264.7 cells for 24 hours, the expressions of protein and mRNA of NLRP3, Caspase-1, and IL-1βwere significantly higher than those of negative control group (P<0.05). The DPI could effectively inhibit the production of ROS.When ROS expression was inhibited, the expressions of NLRP3 inflammatory protein and mRNA were significantly reduced. Conclusion: LTA is the virulence factor of Enterococcus faecalis activating NLRP3 inflammasome, mainly by the production of ROS.

Key words: NLRP3 inflammasome, Enterococcus faecalis, LTA, ROS

中图分类号:

R781

王丽娜, 叶丹丹, 王娇娇, 牛卫东. 粪肠球菌LTA通过促进ROS的高表达活化NLRP3炎性体[J]. 口腔医学研究, 2018, 34(2): 112-116.

WANG Li-na, YE Dan-dan, WANG Jiao-jiao, NIU Wei-dong. Enterococcus Faecalis LTA Activates NLRP3 Inflammasome by Promoting High Expression of ROS[J]. Journal of Oral Science Research, 2018, 34(2): 112-116.

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粪肠球菌LTA通过促进ROS的高表达活化NLRP3炎性体

Enterococcus Faecalis LTA Activates NLRP3 Inflammasome by Promoting High Expression of ROS

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