口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (9): 880-882.

• 基础研究论著 • 上一篇    下一篇

糖基化终末产物对小鼠骨髓间充质干细胞增殖及相关基因P27、cyclinD1 的影响

孙传锋1,邓超2*,印奇志1,柳海2,周嵩琳2,徐清2   

  1. 1. 六安市人民医院口腔科 安徽 六安 237005;
    2. 皖南医学院
  • 收稿日期:2015-03-04 出版日期:2015-09-28 发布日期:2016-05-04
  • 通讯作者: 邓超,E-mail:289991329@qq.com
  • 作者简介:孙传锋(1985~ ),男,安徽六安人,硕士,住院医师,主要从事口腔科的临床治疗工作。
  • 基金资助:
    皖南医学院重点科研项目培育基金(编号:WK2013Z10)

Effects of Advanced Glycation End Products on the Proliferation of Mouse Bone Marrow Mesenchymal Stem Cells and Expression of P27 and Cyclin D1

SUN Chuan-feng1, DENG Chao2, YIN Qi-zhi1, LIU Hai2, ZHOU Song-lin2, XU Qing2   

  1. 1. Department of Stomatology, Liu'an People's Hospital, Liu'an 237005, China;
    2. Wannan Medical College, Wuhu 241002, China
  • Received:2015-03-04 Online:2015-09-28 Published:2016-05-04

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摘要： 目的:探讨糖基化终末产物(AGEs)对小鼠骨髓间充质干细胞(BMSCs)增殖能力以及相关基因P27、cyclinD1的影响。方法:全骨髓法培养小鼠骨髓间充质干细胞;成骨、成脂诱导骨髓间充质细胞,对其进行干细胞鉴定;将培养出的骨髓间充质干细胞与不同浓度的AGEs 共培养,MTT检测不同浓度下骨髓间充质干细胞增殖的改变;实时定量聚合酶链反应(Real time PCR)检测AGEs刺激后P27、cyclin D1表达的改变。结果:小鼠骨髓间充质干细胞成骨诱导21 d后茜素红染色出现钙化结节;成脂诱导21 d后油红O染色出现脂滴;MTT显示不同浓度的AGEs(10、50、100、200 mg/L)均能抑制BMSCs的增殖差异有统计学意义(P<0.05),且随着浓度的增加抑制越明显;Real time PCR结果显示:AGEs(50 mg/L)刺激3 d后P27 mRNA的表达水平较对照组升高,cyclinD1 mRNA的表达水平较对照组降低,差异有统计学意义(P<0.05)。结论:AGEs能抑制小鼠骨髓间充质干细胞的增殖并能改变P27 、cyclinD1 mRNA的表达。

关键词: 糖基化终末产物, 小鼠骨髓间充质干细胞, 增殖

Abstract: Objective: To investigate the effects of advanced glycation end products (AGEs) on the proliferation of mouse bone marrow mesenchymal stem cells (BMSCs). Methods: BMSCs were adopted by whole bone marrow adherence method. Osteogenic differentiation capacity of BMSCs was evaluated by Alizarin red staining. Adipogenic differentiation capacity of BMSCs was evaluated by Oil red staining. After induced with different concentrations of AGEs, the proliferation of BMSCs was assayed by MTT. In addition, real-time quantitative reverse transcription polymerase chain reaction (real-time PCR) was performed to detect the gene expression levels. Results: After 21-day induction, Alizarin red staining showed the formation of mineralization nodules and oil red staining showed the formation of lipid droplets. All the different concentrations of AGEs (10μg/mL, 50μg/mL, 100μg/mL, 200μg/mL) obviously inhibited the proliferation of BMSCs. Meanwhile, the expressions of P27 and cyclinD1 in the experimental group was significantly different from that in the control group (P<0.05). Conclusion: AGEs could decrease the proliferation capacity of BMSCs and regulate the gene expression levels of P27 and cyclin D1.

Key words: Advanced glycation end products, Mouse bone marrow mesenchymal stem cell, Proliferation

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