口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (7): 699-703.DOI: 10.13701/j.cnki.kqyxyj.2016.07.009

• 基础研究论著 • 上一篇    下一篇

抗牙龈素粘附片段Hgp44卵黄抗体对实验性牙周炎的抑制作用

孟明理1, 徐燕1*, 王晓静1, 李增礼2, 周乐春2, 程婷2, 沈继龙3, 徐振山2, 王荣海2   

  1. 1. 安徽医科大学附属口腔医院牙周科,安徽省口腔疾病研究中心实验室 安徽 合肥 230032;
    2. 安徽安科生物工程集团股份有限公司;
    3. 安徽医科大学人兽共患病安徽省重点实验室
  • 收稿日期:2016-01-08 出版日期:2016-07-26 发布日期:2016-07-25
  • 通讯作者: 徐燕,E-mail:173236344@qq.com
  • 作者简介:孟明理(1989~ ),男,安徽人,硕士,主要从事牙周病的研究工作。
  • 基金资助:
    安徽省自然基金(编号:1408085MKL28);安医大安科生物校企合作项目(编号:K2015011);安徽省教育厅2013级研究生"千人培养计划"

Inhibition of Anti-Gingipains Adhesin Domains Hgp44 Yolk Antibody (IgY) for Experimental Periodontitis Induced by Porphyromonas Gingivals.

MENG Ming-li1, XU Yan1*, WANG Xiao-jing1, LI Zeng-li2 , ZHOU Le-chun2 , CHENG Ting2, SHEN Ji-long3, XU Zhen-shan2 ,WANG Rong-hai2.   

  1. 1. Department of Periodontology, Stomatologic Hospital ﹠ College, Anhui Medical University, Key Lab of Oral Diseases Research of Anhui Province, Hefei 230032, China;
    2. Anhui Anke Biotechnology Group Co., Ltd., Hefei 230088, China;
    3. Key Lab of Zoonoses of Anhui Province, Anhui Medical University, Hefei 230032, China
  • Received:2016-01-08 Online:2016-07-26 Published:2016-07-25

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摘要： 目的: 观察抗牙龈素粘附片段Hgp44卵黄抗体(抗Hgp44-IgY)对大鼠实验性牙周炎的抑制作用。方法: 24只雄性SD大鼠随机分为4组:空白组(A),生理盐水孵育组(B),抗Hgp44-IgY孵育组(C)和西吡氯铵孵育组(D)。采用细线结扎大鼠4颗第一磨牙,分别接种经处理的牙龈卟啉单胞菌,辅以蔗糖饮水。4周后检测大鼠牙龈指数(GI),龈下菌斑的BANA试验。处死大鼠后检测牙槽骨丧失量(ABL),牙龈的HE染色,利用qRT-PCR检测牙龈中IL-10、OPG和RANKL mRNA相对表达水平以及OPG/RANKL比率。结果: C组和D组与B组相比,GI、ABL以及BANA结果均显著降低(P<0.01),牙龈组织中IL-10和OPG的mRNA表达水平均显著增高(P<0.01),RANKL mRNA的表达水平显著下降(P<0.05),OPG/RANKL比率显著增高(P<0.001),C组与D组相比,GI、ABL、BANA、IL-10,OPG mRNA水平和OPG/RANKL比率无统计学意义,OPG mRNA 表达水平显著降低(P<0.05)。结论: 抗Hgp44-IgY能减少牙槽骨的吸收,减缓大鼠实验性牙周炎的发展进程。

关键词: 实验性牙周炎, 牙龈素, 卵黄抗体, 牙槽骨丧失

Abstract: Objective: To investigate the inhibiting effect of anti-Hgp44-IgY for experimental periodontitis in rat. Methods: A total of 24 male SD rats were divided randomly into four groups, including the blank group (A), incubation with normal saline (NS) group (B), incubation with anti-Hgp44-IgY group (C) and incubation with cetylpyridinium chloride group (D). Periodontitis was induced by ligation of a silk suture around the neck of the four molar teeth in rats following inoculated with porphyromonas gingivalis. Gingiva index (GI) and BANA test of subgingival bacterial plaque were performed 4 weeks later. Alveolar bone loss (ABL) was detected by dissecting microscope and gum observed by HE stain. The relative mRNA expression levels of IL-10, OPG and RANKL in gums of rats were detected by qRT-PCR and the ratio of OPG/RANKL was calculated. Results: Compared with group B, GI, ABL and BANA were significantly downregulated in both Group C and Group D (P<0.01); while mRNA expression levels of IL-10, OPG and OPG/RANKL ratio were significantly increased in gingival tissue (P<0.01) and the level of RANKL was significantly decreased (P<0.05). The differences of GI, ABL, BANA, IL-10 and RANKL mRNA and OPG/RANKL ratio between Group C and Group D showed no statistical significances; while the OPG mRNA in the Group C was significantly lower than Group D. Conclusion: Anti-Hgp44-IgY can decrease the alveolar bone loss of the experimental periodontitis in rats.

Key words: Experimental periodontitis, Gingipains, Egg yolk antibody, Alveolar bone loss

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