口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (3): 244-248.DOI: 10.13701/j.cnki.kqyxyj.2017.03.003

• 基础研究论著 • 上一篇    下一篇

生命早期负性应激对大鼠实验性牙周炎的影响

刘飞1,2，张林3，肖刚1,2，郭青玉1,2*   

  1. 1. 西安交通大学口腔医学院陕西省牙颌疾病临床研究中心 陕西 西安 710004;
    2. 西安交通大学口腔医院儿童牙病科 陕西 西安 710004;
    3. 河南科技大学第一附属医院口腔科 河南 洛阳 471000
  • 收稿日期:2016-10-25 出版日期:2017-03-20 发布日期:2017-03-22
  • 通讯作者: 郭青玉,电话:029-87215076
  • 作者简介:刘飞(1989~ ),男,陕西西安人,博士在读,研究方向为儿童口腔医学。
  • 基金资助:
    陕西省科技统筹创新工程计划项目(编号:2015KTC03-07)

Effect of Early Life Stress on the Progression of Experimental Periodontitis in Rats.

LIU Fei1,2, ZHANG Lin3, XIAO Gang1,2, GUO Qing-yu1,2*.   

  1. 1. Clinical Research Center of Shanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University. Xi'an 710004, China;
    2. Department of Pediatric Dentistry, College of Stomatology, Xi'an Jiaotong University. Xi'an 710004, China;
    3. Department of Stomatology, First Affiliated Hospital of Henan University of Science and Technology, Luoyang 471000, China
  • Received:2016-10-25 Online:2017-03-20 Published:2017-03-22

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摘要： 目的:探究生命早期负性应激对大鼠成年后实验性牙周炎进展的影响。方法:在3周龄雄性SD大鼠建立慢性温和不可预知应激(unpredictable chronic mild stress, UCMS)模型,以正常饲养大鼠为对照。应激5周后对所有大鼠右上第二磨牙进行实验性牙周炎处理。通过行为学实验评估大鼠情绪状态;ELISA检测血清皮质酮、IL-1β和TNF-α水平;HE染色观察破骨细胞数目,测量牙槽骨丧失量。结果:UCMS大鼠出现糖水偏好下降(P<0.001)、强迫游泳实验中不动时间增加(P<0.01)等抑郁样行为;血清皮质酮及炎性细胞因子水平升高(P<0.05)。HE染色可见UCMS大鼠牙周炎侧牙槽骨吸收量最大,其次为对照组牙周炎侧,再次为对照组正常侧(P<0.001);破骨细胞数目变化趋势与之类似(P<0.001)。血清皮质酮含量与牙周破坏程度呈正相关关系(P<0.05)。结论:生命早期负性应激可能通过HPA轴增强体液免疫,加剧大鼠实验性牙周炎病变程度。

关键词: 生命早期, 应激, 实验性牙周炎, 大鼠

Abstract: Objective: To explore the effect of early life stress on the progression of experimental periodontitis in rats. Methods: Twenty male SD rats of 3 weeks old were divided into two groups randomly. One group was suffered the unpredictable chronic mild stress (UCMS) procedure and the other was the control group. After five weeks' UCMS procedure, all rats received the operation of experimental periodontitis (EP). Then the behavioral tests, sucrose preference test, serum corticosterone (CORT) and inflammatory cytokines by ELISA, and HE staining were performed. Results: The UCMS rats showed the depression-like behaviors, including enhanced immobility time in forced swimming test (P<0.01) and lower sucrose preference test (P<0.001). Serum corticosterone, IL-1β and TNF-α was also elevated (P<0.05). In HE staining, rats with EP showed an obvious alveolar bone loss compared with that in the contra-lateral left side. The alveolar bone loss in the UCMS rats was significantly more severe compared with control group (P<0.001). The same tendency was found in the osteoclast numbers (P<0.001). Positive correlation was found between serum corticosterone and severity of EP (P<0.05). Conclusion: Early life stress could aggravate the progression of EP, which might be regulated by HPA axis and humoral immunity.

Key words: Early life, Stress, Experimental periodontitis, Rats

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