EPO对唾液腺放射性损伤防护作用的研究

doi:10.13701/j.cnki.kqyxyj.2017.06.006

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (6): 601-604.DOI: 10.13701/j.cnki.kqyxyj.2017.06.006

EPO对唾液腺放射性损伤防护作用的研究

王越^1,2, 孙宾¹, 马晓周¹, 柳康¹, 杜宝霞¹, 于阳¹, 张伟^1*

1. 吉林大学口腔医学院吉林长春 130021;
2. 吉林省牙发育及颌骨重塑与再生重点实验室吉林长春 130021)

收稿日期:2016-11-25 出版日期:2017-06-20 发布日期:2017-06-26
通讯作者: 张伟,E-mail:doctor zhang 888@sina.com
作者简介:王越(1990~ ),男,山东人,硕士在读,主要从事唾液腺相关疾病研究。
基金资助:
吉林省直厅局项目(编号:5139073431)

Protective Effect of Erythropoietin against Salivary Gland Radiation Injury.

WANG Yue^1,2, SUN Bin¹, MA Xiao-zhou¹, LIU Kang¹, DU Bao-xia¹, YU Yang¹, ZHANG Wei^1*.

1. School and Hospital of Stomatology, Jilin University, Changchun 130021, China;
2. Provincial Key Laboratory of Tooth Development and Jaw Remodeling and Regeneration, Changchun 130021,China.

Received:2016-11-25 Online:2017-06-20 Published:2017-06-26

摘要/Abstract

摘要： 目的:通过研究促红细胞生成素(Erythropoietin,EPO)对大鼠下颌下腺放射性损伤的防护作用,为临床进一步探索唾液腺放射性损伤的预防和保护提供实验基础。 方法:将30只Wistar大鼠平均分为3组,即对照组(模拟照射+注射盐水)、单放组(照射+注射盐水)、给药组(照射+注射药物)。大鼠头颈部一次性接受15 Gy大剂量放射线照射,给药组放射前30 min腹腔注射EPO,照射后隔日给药,持续2周,药物剂量为每次3000 IU/kg。放射后第90天测量大鼠唾液流率,测量完毕后取大鼠颌下腺制作石蜡切片,行HE染色、Masson染色,并通过免疫组织化学染色观察切片PCNA表达情况。结果:给药组大鼠唾液流率大于单放组,组织学观察给药组腺体损伤程度较单放组明显减轻,每高倍视野(×400)给药组PCNA阳性细胞数多于单放组。结论:EPO对唾液腺放射性损伤有较好的防护作用。

关键词: EPO, 放射防护, 大鼠, 颌下腺

Abstract: Objective: To investigate the protection of erythropoietin (EPO) against the radiation-induced salivary glands injury in rats. Methods: Thirty rats were divided into 3 groups, which were the control group, radiation group (IR+saline), and treatment (IR+EPO) group. The rat's head and neck received a single dose of 15Gy. EPO treatment via intraperitoneal was started 30min before irradiation and then was repeated every 48h for the first 2 weeks at dose of 3000IU/Kg at the end of irradiation. The salivary flow rate was assessed after 90 days and then the submandibular was removed to make Paraffin for HE staining, Masson staining, and immunohistochemical staining to observe the expression of PCNA. Results: The IR+EPO group's salivary secretion was greater than that of IR+saline group and exhibited less injury of gland tissue than the IR+saline group. The control group showed a higher PCNA positive cell rate than the IR+saline group. Conclusion: EPO has a good protective effect against the radiation-induced salivary gland injury.

Key words: EPO , Radiation protection, Rats , Submandibular gland

中图分类号:

R816.98

王越, 孙宾, 马晓周, 柳康, 杜宝霞, 于阳, 张伟. EPO对唾液腺放射性损伤防护作用的研究[J]. 口腔医学研究, 2017, 33(6): 601-604.

WANG Yue, SUN Bin, MA Xiao-zhou, LIU Kang, DU Bao-xia, YU Yang, ZHANG Wei.. Protective Effect of Erythropoietin against Salivary Gland Radiation Injury.[J]. Journal of Oral Science Research, 2017, 33(6): 601-604.

参考文献

[1] Yoshihisa Tateshi, Eri sasabe, Eisaku Ueta, et al. Ionizing irradiation induces apoptotic damage of salivary gland acinar cells via NADPH oxidase 1-dependent superoxide generation [J]. Biochemical and Biophysical Research Communications, 2008, 366 (2)∶301-307
[2] Mana Kamiya, Tomoyuki Kawase, Kazuhide Hayama, et al. X-Ray-Induced Damage to the Submandibular Salivary Glands in Mice: An Analysis of Strain-Specific Responses [J]. BioResearch Open Access, 2015, 4(1)∶307-308
[3] M Imanimoghaddam, M Rahrooh, Z Tafakhori, et al. Changes of parotid and Submandibular glands caused by radiotherapy-an ultrasound evaluation [J]. Dentoma- xillofacial Radiology, 2012,41(3)∶379-384
[4] Raluca Stiubea-Cohen, Ran David, Yoav Neumann, et al. Effect of Irradiation on Cell Transcriptome and Proteome of Rat Submandibular Salivary Glands [J]. Plos One, 2012, 7(1)∶1-9
[5] Kumral A, Baskin H, Gokmen N, et al. Selective inhibition of nitric oxide in hypoxic-ischemic brain model in newborn rats: Is it an explanation for the protective role of erythropoietin [J]? Biol Neonate, 2004, 85(1)∶51-54
[6] Katavetin P, Tungsanga K, Eiam-Ong S, NangakuM. Antioxidative effects of erythropoietin [J]. Kidney International, 2007, 72(1)∶10-15
[7] Campana WM, Myers RR. Exogenous erythropoietin protects against dorsal root ganglion apoptosis and pain following peripheral nerve injury [J]. Eur J Neurosci, 2003, 18(6)∶1497-1506
[8] Marti HH, Bernaudin M, Petit E, et al. Neuron-protection and angiogenesis: a dual role of erythropoietin in brain ischemia [J]. News Physical Sci, 2000, 15(5)∶225-229
[9] Nagler RM, The enigmatic mechanism of irradiation-induced damage to the major salivary glands [J]. Oral disease, 2002, 8(3)∶141-146
[10] Miranda Muhvic Urek, Marina Bralic, Jelena Tomac, et al. Early and Late Effects of X-Irradiation on Submandibular Gland:A Morphological Study in Mice [J]. Archives of Medical Research, 2005, (36)∶339-343
[11] Jun LI, Zhaochen SHAN, Guangfei OU, et al. Structural and functional characteristic of irradiation damage to parotid gland in the miniature pig [J]. Int J Radiat Oncol Biol Phys, 2005, 62(5)∶1510-1516
[12] Rob P. Coppesa, Arjan Vissinkc, Antonius W. T. Konings, Comparison of radiosensitivity of rat parotid and submandibular glands after different radiation schedules [J]. Radiotherapy and Oncology, 2002 (63)∶321-328
[13] Bin Xiang, Xiu-xiu Li, Fu-yin Zhang. Underlying protective mechanism of a1-adrenoceptor activation against irradiation-induced damage in rat submandibular gland [J]. archives of oral biology, 2013(58)∶1238-1245

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EPO对唾液腺放射性损伤防护作用的研究

Protective Effect of Erythropoietin against Salivary Gland Radiation Injury.

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