放疗对小型猪腮腺颌下腺舌下腺微血管损伤影响的实验研究

doi:10.13701/j.cnki.kqyxyj.2017.01.004

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (1): 15-18.DOI: 10.13701/j.cnki.kqyxyj.2017.01.004

放疗对小型猪腮腺颌下腺舌下腺微血管损伤影响的实验研究

颜兴^1*,武鸿毅²,许诺¹,孟岑¹

1. 首都医科大学附属北京友谊医院口腔科北京 100050;
2. 北京市隆福医院口腔科北京 100010

收稿日期:2016-06-03 出版日期:2017-01-25 发布日期:2017-01-22
通讯作者: 颜兴,E-mail: yanxing_jy@aliyun.com
作者简介:颜兴(1972~ ),浙江绍兴人,博士后,副主任医师,主要从事口腔干细胞,牙源性iPS细胞,涎腺放射性损伤,牙槽外科研究工作。
基金资助:
国家自然科学基金(编号:81070844)
北京市卫生系统高层次卫生技术人才培养计划项目(编号:2013-3-065)

Effect of Radiotherapy on Microvascular Injury of Parotid, Submandibular and Sublingual Gland in Minipig.

YAN Xing^1*, WU Hong-yi², XU Nuo¹, MENG Cen¹

YAN Xing^1*, WU Hong-yi², XU Nuo¹, MENG Cen¹. 1. Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;
2. Department of Stomatology, Beijing Longfu Hospital, Beijing 100010, China

Received:2016-06-03 Online:2017-01-25 Published:2017-01-22

摘要/Abstract

摘要： 目的:观察放疗对小型猪腮腺、颌下腺、舌下腺的微血管损伤状况。方法:将6只实验用小型猪分为2组。2组动物进行放疗,将双侧腮腺、颌下腺、舌下腺加入放射野中,放疗组20Gy/每侧,对照组0Gy/每侧。放疗结束后4 h处死两组动物,取两组动物腮腺、颌下腺、舌下腺标本行HE染色与CD31免疫组化染色,观察放疗早期3种腺体微血管密度变化。结果:两组小型猪腮腺、颌下腺、舌下腺CD31阳性染色颗粒数有显著差异(P＜0.05);3个腺体间统计结果有显著性差异(P＜0.05);腮腺与颌下腺及舌下腺有显著性差异(P＜0.05),颌下腺与舌下腺无显著性差异。结论:放疗可导致小型猪腮腺、颌下腺、舌下腺微血管密度明显减低,且各腺体间微血管密度减低有差异,腮腺的损伤程度大于颌下腺和舌下腺。

关键词: 腮腺, 颌下腺, 舌下腺, 放射性损伤

Abstract: To observe the microvascular injury of parotid, submandibular and sublingual gland under radiotherapy in minipig. Methods: Six minipigs were divided into two groups (n=3), which were treated by radiotherapy at 0 Gy or 20 Gy at bilateral parotid, submandibular and sublingual glands. After radiotherapy for 4 hours, the minipigs were euthanatized, while their parotid, submandibular and sublingual glands were collected for hematoxylin-eosin (HE) staining and immunohistochemical staining of CD31. Results: There were significant difference in CD31 positive staining between these two groups and among three glands (P<0.05). In addition, CD31 positive staining showed significantly differents between parotid with submandibular or sublingual gland (P<0.05), but no differences were detect beween between submandibular and sublingual gland. Conclusion: Radiotherapy can decrease the microvascular density in the parotid, submandibular and sublingual glands of minipig, but the decreased extent in these glands were different, which in parotid is the most obvious.

Key words: Parotid, gland, Submandibular, gland, Sublingual, gland, Radiation, injury

中图分类号:

R782.05

颜兴,武鸿毅,许诺,孟岑. 放疗对小型猪腮腺颌下腺舌下腺微血管损伤影响的实验研究[J]. 口腔医学研究, 2017, 33(1): 15-18.

YAN Xing, WU Hong-yi, XU Nuo, MENG Cen. Effect of Radiotherapy on Microvascular Injury of Parotid, Submandibular and Sublingual Gland in Minipig.[J]. Journal of Oral Science Research, 2017, 33(1): 15-18.

参考文献

[1] Miah AB, Schick U, Bhide SA, et al. A phase II trial of induction chemotherapy and chemo-IMRT for head and neck squamous cell cancers at risk of bilateral nodal spread: the application of a bilateral superficial lobe parotid-sparing IMRT technique and treatment outcomes [J]. Br J Cancer. 2015, 112(1)∶32-38
[2] 武艳飞,何奎芳.放射性龋的研究现状[J].口腔医学研究,2015,32(7)∶742-744
[3] Cotrim AP, Sowers A, Mitchell JB, et al. Prevention of Irradiation- induced Salivary Hypofunction by Microvessel Protection in Mouse Salivary Glands [J]. Mol Ther, 2007, 15(12)∶2101- 2106
[4] Cooper JS, Fu K, Mark sJ, et al. Late effects of radiationtherapy in the head and neck region [J]. Int J Radiat Oncol Biol Phys, 1995, 31(10)∶1141-1161
[5] Liem IH, Valdes-Olmos RA, Balm AJM, et al. Evidence for early and persistent impairment of salivary gland excretion after irradiation of head and neck tumours [J]. Eur J Nucl Med, 1996, 23(12)∶1485-1490
[6] Valdez IH, Atkinson JC, Ship JA, et al. Major salivary gland function in patients with radiation-induced xerostomia: Fow rates and sialochemistry [J]. Int J Radiat Oncol Biol Phys, 1992, 25(1)∶ 41-47
[7] Burlage FR, Coppes RP, Meertens H, et al. Parotid and submandibular/sublingual salivary flow during high dose radiotherapy [J]. Radiother Oncol, 2001, 61(3)∶271-274
[8] Coppes RP, Vissink A, Konings AW. Comparison of radiosensitivity of rat parotid and submandibular glands after different radiation schedules [J]. Radiother Oncol, 2002, 63(3)∶321-328
[9] Vissink A, Kalicharan D, s-Gravenmade EJ, et al. Acite irradiation effects on morphology and function of rat submandibular glands [J]. J Oral Pathol Med, 1991, 20(4)∶449-456
[10] Nagler TM, Short- and long-term functional vs morphometrical salivary effects of irradiation in a rodent model [J]. Anticancer Res, 1998, 18(3)∶315-320
[11] 张昕,王松灵,李钧,等.5种哺乳动物涎腺造影及解剖比较研究[J].北京口腔医学杂志,2003,11(4)∶195-199
[12] Yan X, Hai B, Shan ZC, et al.. Effect of Same-dose Single or Dual Field Irradiation on Damage to Miniature Pig Parotid Glands [J]. International Journal of Oral Science, 2009, 1(1)∶16-26
[13] J Xu, X Yan, R Gao, et al. Effect of irradiation on microvascular endothelial cells of parotid glands in the miniature pig [J]. Int J Radiat Oncol Biol Phys, 2010, 78(3)∶897-903
[14] 霍文艳,颜兴,郝龙英,等.血管内皮生长因子对小型猪腮腺放射性损伤影响的实验研究[J].中华老年口腔医学杂志, 2010,8(2)∶102-106
[15] Rocha EM, Cotrim AP, Zheng C. Recovery of radiation-induced dry eye and corneal damage by pretreatment with adenoviral vector-mediated transfer of erythropoietin to the salivary glands in mice [J]. Hum Gene Ther, 2013, 24(4)∶417-423

放疗对小型猪腮腺颌下腺舌下腺微血管损伤影响的实验研究

Effect of Radiotherapy on Microvascular Injury of Parotid, Submandibular and Sublingual Gland in Minipig.

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