小鼠切牙损伤对成牙本质细胞Notch表达和细胞凋亡的影响

doi:10.13701/j.cnki.kqyxyj.2016.08.002

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (8): 780-784.DOI: 10.13701/j.cnki.kqyxyj.2016.08.002

小鼠切牙损伤对成牙本质细胞Notch表达和细胞凋亡的影响

尹秋蓉¹,王胜朝^1*,罗颖²,童娟¹,胡轶¹

1. 第四军医大学口腔医学院口腔预防科,军事口腔医学国家重点实验室陕西西安 710032;
2. 第四军医大学病理生理学教研室陕西西安 710032

收稿日期:2015-04-10 出版日期:2016-08-26 发布日期:2016-08-26
通讯作者: 王胜朝,电话:029-84776479
作者简介:尹秋蓉(1983~ ),女,四川广安人,硕士,主要从事口腔预防医学的相关工作。
基金资助:
国家自然科学基金项目(编号:81170947)

Effect of Mechanical Injury on the Expression of Notch Signaling and Apoptosis in Odontoblasts of Mouse Incisor.

YIN Qiu-rong¹, WANG Sheng-chao¹, LUO Ying², TONG Juan¹, HU Yi¹

1. State Key Laboratory of Military Stomatology, Department of Preventive Dentistry, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China;
2. Department of Pathophysiology, The Fourth Military Medical University, Xi’an 710032, China

Received:2015-04-10 Online:2016-08-26 Published:2016-08-26

摘要/Abstract

摘要： 目的:观察小鼠切牙机械损伤后成牙本质细胞(OB)的Notch表达及细胞凋亡情况,分析Notch信号与OB细胞凋亡的可能关系。方法:小鼠下颌切牙牙冠远中颈部机械备洞制造牙齿损伤,损伤后1、3、5、7 d取材,用免疫组织化学染色方法检测OB细胞Notch1、Notch2表达情况,用TUNEL染色法观察OB细胞凋亡情况。结果:机械损伤可激活OB细胞Notch表达并呈动态变化:损伤后1 d,Notch1、Notch2呈阳性表达;损伤后3d,Notch1、Notch2表达最明显,呈强阳性;损伤后5 d,Notch1、Notch2仍呈阳性表达;损伤后7 d,Notch1、Notch2表达均减弱。对照组OB细胞Notch1、Notch2仅有微弱表达。细胞凋亡检测显示:对照组OB层仅见零星凋亡细胞;损伤组的近损伤区凋亡细胞明显增多。OB细胞凋亡在损伤后1 d最多,损伤后3~7 d逐渐减少。以上说明:机械损伤导致OB层细胞凋亡和Notch表达激活伴行出现,前者1 d最明显,后者3 d达高峰。结论:小鼠切牙机械损伤后成牙本质细胞Notch信号表达激活并可能与细胞凋亡调控存在联系。

关键词: 成牙本质细胞, Notch信号, 细胞凋亡, 机械损伤

Abstract: Objective: To investigate Notch expression and apoptosis in odontoblasts of mouse incisor after mechanical injury. Methods: Dental mechanical injury model was established through the cavity preparation on the mandibular incisor of mice. The expressions of Notch1 and Notch2 in the odontoblasts were observed by immunohistochemical staining, and apoptosis of the odontoblasts was observed by TUNEL staining. Results: The expression of Notch in odontoblasts were activated by mechanical injury and showed a dynamic change. One day after injury, the expressions of Notch1 and Notch2 were positive; 3 days after injury, the expressions of Notch1 and Notch2 were significantly increased and were intensively positive; 5 days after injury, Notch1 and Notch2 were still positive; 7 days after injury, the expressions of Notch1 and Notch2 became weak. In control, expressions of Notch1 and Notch2 were very weak. Mechanical injury leaded to massive apoptosis in dental pulp. Apoptosis staining in the odontoblasts was obvious 1 day after injury, and the apoptotic cells decreased gradually from 3-7 days after injury. Few apoptotic odontoblasts were observed in the control group. Concomitant pattern of the cell apoptosis and Notch expression in the odontoblasts were exhibited after mechanical injury of tooth. Conclusion: Dynamic activation of Notch signaling might be connected with apoptosis regulation in odontoblasts after mechanical injury.

Key words: Odontoblast, Notch signaling, Apoptosis, Mechanical injury

中图分类号:

R780.1

尹秋蓉,王胜朝,罗颖,童娟,胡轶. 小鼠切牙损伤对成牙本质细胞Notch表达和细胞凋亡的影响[J]. 口腔医学研究, 2016, 32(8): 780-784.

YIN Qiu-rong, WANG Sheng-chao, LUO Ying, TONG Juan, HU Yi. Effect of Mechanical Injury on the Expression of Notch Signaling and Apoptosis in Odontoblasts of Mouse Incisor.[J]. Journal of Oral Science Research, 2016, 32(8): 780-784.

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小鼠切牙损伤对成牙本质细胞Notch表达和细胞凋亡的影响

Effect of Mechanical Injury on the Expression of Notch Signaling and Apoptosis in Odontoblasts of Mouse Incisor.

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