口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (3): 216-219.DOI: 10.13701/j.cnki.kqyxyj.2016.03.002

• 基础研究论著 • 上一篇    下一篇

S1P/S1PR1通路依赖的压力对破骨前体细胞趋化效应

方洁1, 叶茜1, 李宇红1, 2*, 黄声富1, 3*   

  1. 1. 武汉大学口腔医学院口腔基础医学省部共建国家重点实验室培训基地和口腔生物医学教育部重点实验室 湖北 武汉 430079;
    2. 武汉大学口腔医院牙体牙髓科 湖北 武汉 430079;
    3. 武汉大学口腔医院正畸科 湖北 武汉 430079
  • 收稿日期:2016-02-01 出版日期:2016-03-28 发布日期:2016-03-29
  • 通讯作者: 李宇红,电话: 027-87686007
    黄声富,电话:027-87686110
  • 作者简介:方洁(1990~ ),女,安徽合肥人,硕士在读,研究方向为牙体牙髓病学。
  • 基金资助:
    国家自然科学基金项目(编号:81271190)

S1P/S1PR1 Regulates Chemotaxis of Osteoclast Precursors under Compressive Stress.

FANG Jie1, YE Xi1, LI Yu-hong1,2*, HUANG Sheng-fu1,3*   

  1. 1. The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China;
    2. Department of Endodontics, School of Stomatology, Wuhan University, Wuhan 430079, China;
    3. Department of Orthodontics, School of Stomatology, Wuhan University, Wuhan 430079, China
  • Received:2016-02-01 Online:2016-03-28 Published:2016-03-29

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摘要： 目的: 探究S1P/S1PR1通路轴在压力下对破骨前体细胞趋化影响的调节作用。方法: 建立体外压力模型,通过实时荧光定量聚合酶链式反应,免疫印迹实验和细胞免疫荧光检测压力下Raw264.7细胞SPHK1和S1PR1的mRNA水平和蛋白水平。采用Transwell 24孔板通过趋化实验探究S1P/S1PR1在压力下对Raw264.7细胞的迁移能力的影响。结果: 发现0.5、1.0和2.0 g/cm2压力作用下,Raw264.7细胞的SPHK1和S1PR1的表达及Raw264.7细胞的趋化显著降低。在S1PR1受体功能性激动剂FTY720作用下,压力作用下趋化能力被抑制的Raw264.7细胞趋化水平显著增加。结论: S1P/S1PR1信号轴在正畸治疗中调节破骨前体细胞迁移与功能发挥重要作用。
[关键词] S1P/S1PR1通路 压力 破骨前体细胞 趋化

关键词: S1P/S1PR1通路, 压力, 破骨前体细胞, 趋化

Abstract: Objective: To investigate the role of Sphingosine-1-phosphate/Sphingosine-1-phosphate receptor 1 (S1P/S1PR1) axis in regulating migration of osteoclast precursors under compressive stress. Methods: Compressed model was built in vitro. The mRNA and protein expression levels of SPHK1 and S1PR1 in Raw 264.7 cell lines under compressive stress were investigated with real-time PCR, Western-blotting and immunofluoresecence. The regulatory role of S1P/S1PR1 signaling on chemotaxis of Raw 264.7 cell lines under compressive stress was explored. Results: Under compressive force of 0.5, 1.0 and 2.0 g/cm2, SPHK1 and S1PR1 expressions and chemotaxis of Raw 264.7 cell lines were downregulated. And S1P/S1PR1 signaling functional antagonist FTY720 increased the chemotaxis of Raw 264.7 cells. Conclusion: S1P/S1PR1 axis regulates migration of osteoclast precursors under compressive stress.

Key words: S1P/S1PR1 axis, Compressive stress , Osteoclast precursors , Migration

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