具核梭杆菌调控牙龈卟啉单胞菌感染对口腔鳞状细胞癌KB细胞周期和细胞因子分泌的研究

doi:10.13701/j.cnki.kqyxyj.2016.03.001

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (3): 211-215.DOI: 10.13701/j.cnki.kqyxyj.2016.03.001

• 基础研究论著 • 下一篇

具核梭杆菌调控牙龈卟啉单胞菌感染对口腔鳞状细胞癌KB细胞周期和细胞因子分泌的研究

王晴萱, 刘俊超, 潘亚萍^*

中国医科大学口腔医学院·中国医科大学附属口腔医院牙周病科辽宁沈阳 110002

收稿日期:2015-11-19 出版日期:2016-03-28 发布日期:2016-03-29
通讯作者: 潘亚萍,E-mail: yppan@mail.cmu.edu.cn
作者简介:王晴萱(1992~ ),女,辽宁人,学士,主要从事牙周病学的研究工作。
基金资助:
国家自然科学基金资助项目(编号:81271153和81470745)

Impact of Fusobacterium Nucleatum and Porphyromonas Gingivalis Modulation on the Cell Cycle and Cytokines Production of KB Cells.

WANG Qing-xuan, LIU Jun-chao, PAN Ya-ping^*

School of Stomatology, China Medical University, Shenyang 110002, China

Received:2015-11-19 Online:2016-03-28 Published:2016-03-29

摘要/Abstract

摘要： 目的: 通过研究牙龈卟啉单胞菌(Porphyromonasgingivalis, P. gingivalis)和具核梭杆菌(Fusobacteriumnucleatum, F. nucleatum)感染对KB细胞的细胞周期和炎症因子分泌的调控,初步探讨牙周细菌感染对口腔鳞状细胞癌进展和炎症反应的影响。方法: 建立P. gingivalis和F. nucleatum单独或联合感染KB细胞模型,透射电镜观察细菌感染细胞情况,流式细胞术检测细胞周期变化,酶联免疫法检测IL-6和IL-8蛋白分泌水平。结果: P. gingivalis联合F. nucleatum感染KB细胞8 h抑制细胞周期进程,感染24 h后加速细胞周期进程;P. gingivalis联合F. nucleatum感染促进KB细胞分泌IL-6和IL-8,并且有时间和F. nucleatum浓度依赖性。结论: 牙周致病菌之间的联合作用更能促进口腔鳞状细胞癌的发展及免疫炎症反应。

关键词: 具核梭杆菌, 牙龈卟啉单胞菌, 细胞周期, 细胞因子, KB细胞

Abstract: Objective: To investigate the effects of P. gingivalis/F. nucleatum on the cell cycle and cytokines production of KB cells.Methods: In vitro models were established with KB cells co-cultured with P. gingivalis/F. nucleatum either alone or in combination. Transmission electron microscopy was used to observed invasive ability, the change of cell cycle of KB cells was detected by flow cytometry, and the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) was analyzed by enzyme-linked immunoadsordent assay. Results: Eight-hour co-infection with P. gingivalis/F. nucleatum slowed the cell cycle of KB cells, while eight-hour co-infection accelerated the cell cycle. The production of IL-6 and IL-8 was significantly up-regulated after co-infection with P. gingivalis/F. nucleatum in a time dependent and F. nucleatum density related manner. Conclusion: Combination of oral pathogens can stimulate tumorigenesis and inflammation response in OSCC.

Key words: Fusobacterium nucleatum , Porphyromonas gingivalis , Cytokines , Cell cycle, KB cells

中图分类号:

R781.4

王晴萱, 刘俊超, 潘亚萍. 具核梭杆菌调控牙龈卟啉单胞菌感染对口腔鳞状细胞癌KB细胞周期和细胞因子分泌的研究[J]. 口腔医学研究, 2016, 32(3): 211-215.

WANG Qing-xuan, LIU Jun-chao, PAN Ya-ping. Impact of Fusobacterium Nucleatum and Porphyromonas Gingivalis Modulation on the Cell Cycle and Cytokines Production of KB Cells.[J]. Journal of Oral Science Research, 2016, 32(3): 211-215.

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具核梭杆菌调控牙龈卟啉单胞菌感染对口腔鳞状细胞癌KB细胞周期和细胞因子分泌的研究

Impact of Fusobacterium Nucleatum and Porphyromonas Gingivalis Modulation on the Cell Cycle and Cytokines Production of KB Cells.

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