口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (3): 220-223.DOI: 10.13701/j.cnki.kqyxyj.2016.03.003

• 基础研究论著 • 上一篇    下一篇

LPS刺激小鼠骨细胞RANKL/OPG和IL-6表达的实验研究

余科1, 吴湘楠2, 刘文佳2, 王航2*   

  1. 1. 泸州医学院附属口腔医院修复科 四川 泸州 646000;
    2. 四川大学华西口腔医院修复科 四川 成都 610041
  • 收稿日期:2015-10-20 出版日期:2016-03-28 发布日期:2016-03-29
  • 通讯作者: 王航,E-mail:hwang1999@163.com
  • 作者简介:余科（1979~ ），男，四川崇州人，讲师，博士，主要从事口腔医学临床及基础研究。
  • 基金资助:
    国家自然科学基金资助项目(编号:81170941)

Effects of LPS on Expression of RANKL/OPG and IL-6 in Mouse Osteocytes in Vitro.

YU Ke1,WU Xiang-lan2,LIU Wen-jia2,WANG Hang2*   

  1. 1. Department of Prosthodontics,Hospital of Stomatology Affiliated to Luzhou Medical College, Luzhou 646000,China;
    2. Department of Prosthodontics,West China College of Stomatology Affiliated to Sichuan University, Chengdu 610041,China
  • Received:2015-10-20 Online:2016-03-28 Published:2016-03-29

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2

摘要： 目的: 探讨LPS对体外培养的小鼠MLO-Y4细胞RANKL/OPG和IL-6表达的影响。方法: 以5 mg/L的LPS刺激细胞,用CCK-8法于(12、24、48 h)后检测细胞的增殖;以不同浓度(1、10、100、500、1000 μg/L)的LPS刺激细胞,分别在作用4 h和1.5 h后用RT-PCR检测细胞对RANKL/OPG和IL-6的相对表达;以100 μg/L的LPS刺激细胞,分别在作用(0.5、1、2、4、8 h)和(0.5、1、1.5、2、4 h)两种不同时间后用RT-PCR检测细胞对RANKL/OPG和IL-6的相对表达。结果: LPS对MLO-Y4细胞增殖无影响;100 μg/L的LPS能显著上调细胞对RANKL和IL-6的相对表达,与(500, 1000 μg/L)LPS的上调结果无统计学差别;除0.5 h外其余时间点LPS均上调细胞对RANKL和IL-6的相对表达,并分别在4 h和1.5 h达到峰值;所有样本LPS对细胞OPG的相对表达均无影响。结论: 一定浓度的 LPS上调了MLO-Y4 细胞对RANKL和IL-6的表达,而对OPG的表达无影响。

关键词: 脂多糖, 骨细胞, RANKL, OPG, IL-6

Abstract: Objective: To observe the effects of LPS on the expression of RANKL/OPG and IL-6 in MLO-Y4 cells in vitro. Methods: Cells were stimulated with 5 mg/L LPS and the proliferation of the cells was observed at different time points (12, 24 and 48 h) by CCK-8. Then cells were stimulated with various concentrations of LPS (1, 10, 100, 500 and 1000 μg/L) and RT-PCR was performed to detect the relative expression of RANKL/OPG and IL-6 after 4 h and 1.5 h, respectively. Cells were stimulated with 100 μg/L LPS and relative expression of RANKL/OPG and IL-6 was detected at different time points (0.5, 1, 2, 4 and 8 h) or (0.5, 1, 1.5, 2 and 4 h) respectively by RT-PCR. Results: There was no influence of LPS on the proliferation of MLO-Y4 cells. Relative expression of RANKL and IL-6 was significantly up-regulated in MLO-Y4 cells stimulated with 100, 500 and 1000 μg/L LPS, respectively. When stimulated with 100 μg/L LPS, relative expression of RANKL and IL-6 was up-regulated at all time points except 0.5 h, and RANKL reached peak at 4 h, while IL-6 at 1.5 h. There was no influence of LPS on relative expression of OPG in all samples. Conclusion: Expression of RANKL and IL-6 was up-regulated in MLO-Y4 cells in the presence of a certain concentration of LPS, however, OPG was not intervened.

Key words: Lipopolysaccharides, Osteocyte , RANKL, OPG , IL-6

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