口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (4): 322-325.DOI: 10.13701/j.cnki.kqyxyj.2016.04.002

• 基础研究论著 • 上一篇    下一篇

BMP7对三维条件下小鼠iPS细胞成骨能力的影响

徐冉1,张宇2#,邢舰誉1,王屹博1,丁超1,吕嘉伟2,伟人悦2,史久慧1*,刘忠华2*   

  1. 1. 哈尔滨医科大学附属第一医院口腔科 黑龙江 哈尔滨 150006;
    2. 东北农业大学生命科学学院 黑龙江 哈尔滨 150030
  • 收稿日期:2015-10-13 出版日期:2016-04-28 发布日期:2016-04-28
  • 通讯作者: 史久慧,E-mail:tsjj926@163.com刘忠华,E-mail:liu086@126.com#为共同第一作者
  • 作者简介:徐冉(1989~ ),女,山东莱州市人,硕士,主要从事成骨、iPS的研究工作。
    张宇(1983~ ),女,哈尔滨市人,博士,助理研究员,主要从事成骨、iPS的研究工作。
  • 基金资助:
    国家自然科学基金(项目号:31301211)哈尔滨医科大学心肌缺血省部共建教育部重点实验室开放课题基金(项目号:KF201317)东北农业大学青年才俊项目资助(项目号:14QC01)

Effect of BMP7 on Osteogenic Differentiation of Mouse Induced Pluripotent Stem Cells in 3D Culture System.

XU Ran1, ZHANG Yu2#, XING Jian-yu1, WANG Yi-bo1, DING Chao1, LV Jia-wei2, WEI Ren-yue2, SHI Jiu-hui1*, LIU Zhong-hua2*.   

  1. 1. The First Affiliated Hospital to Harbin Medical University, Harbin 150006, China;
    2. College of Life Science, Northeast Agricultural University, Harbin 150030, China
  • Received:2015-10-13 Online:2016-04-28 Published:2016-04-28

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198

被引次数

2

摘要： 目的:探讨明胶海绵作为体外成骨支架材料的可行性及BMP7对小鼠诱导型多能干细胞(miPSCs)在三维培养条件下骨向分化能力的影响。方法:miPSCs经体外培养形成拟胚体(EBs),将消化所得的单细胞于明胶海绵上成骨诱导,7 d后检测其成骨能力及贴附情况。结果:miPSCs可以贴附并沿着明胶纤维伸展;BMP7添加组的钙结节含量及成骨基因表达水平高于未添加组。结论:明胶海绵可以作为miPSCs体外成骨的培养支架;BMP7对三维培养条件下miPSCs的成骨能力有促进作用。

关键词: 诱导型多能干细胞, 成骨分化, BMP7, 3D

Abstract: Objective: To investigate the effect of BMP7 on the osteogenic differentiation ability of mouse iPSCs in the absorbable gelatin sponge scaffold. Methods: EBs induced from mouse iPSCs were digested into single cells and microinjected into the gelatin sponge. After 7 days of osteogenic induction, the osteogenic and adhesive abilities of the cells were examined. Results: Mouse iPSCs attached and spread along the gelatin sponge. Compared with the group without BMP7 treatment, the group treated with BMP7 had higher levels of deposited calcium and osteoblastic markers. Conclusion: Gelatin sponge can be used as cell culture scaffold for mouse iPSCs and BMP7 promotes osteogenesis of mouse iPSCs in the culture system.

Key words: Mouse

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