口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (5): 445-448.DOI: 10.13701/j.cnki.kqyxyj.2016.05.005

• 基础研究论著 • 上一篇    下一篇

信号素3A对成牙骨质细胞系OCCM-30增殖和矿化功能影响的实验研究

邱雨1,2 ,任嫒姝2,3 ,庞琴1,2 ,马小娟1,2 ,付钢1,4*   

  1. 1. 重庆医科大学附属口腔医院种植科 重庆 401147;
    2. 口腔疾病与生物医学重庆市重点实验室 重庆 401147;
    3. 重庆医科大学附属口腔医院正畸科 重庆 401147;
    4. 重庆市高校市级口腔生物医学工程重点实验室 重庆 401147
  • 收稿日期:2015-09-16 出版日期:2016-05-26 发布日期:2016-05-26
  • 通讯作者: 付钢,E-mail:fg.ras@hotmail.com
  • 作者简介:邱雨(1990~ ),女,湖北人,硕士,主要从事口腔修复学工作。
  • 基金资助:
    重庆市自然科学基金资助项目(CSTC,2011jjA10086)
    重庆市渝中区科委资助项目( 20140128)
    2013年重庆高校创新团队建设计划资助项目

Effects of Sema3A on the Proliferation and Mineralization of a Cementoblast Cell Line OCCM-30.

QIU Yu1,2, REN Ai-shu2,3, PANG Qin1,2, MA Xiao-juan1,2, FU Gang1,4*.   

  1. 1. Department of Implant Dentistry, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China;
    2. Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing 401147, China;
    3. Department of Orthodontics, Affiliated Hospital of Stomatology, Chongqing Medical University , Chongqing 401147, China;
    4. Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China
  • Received:2015-09-16 Online:2016-05-26 Published:2016-05-26

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摘要： 目的:初步探讨信号素3A(Semaphorin3A, Sema3A)对成牙骨质细胞增殖、矿化的影响。方法:采用不同浓度的重组人Sema3A(0、0.5、1 mg/L)处理成牙骨质细胞OCCM-30。MTT法检测Sema3A对成牙骨质细胞增殖的影响,酶比活性法检测细胞碱性磷酸酶(ALP)活性的变化,实时荧光定量 PCR法检测骨钙素(OCN),骨桥蛋白(OPN)和Runx2基因的表达,茜素红染色法检测矿化结节的形成。结果:Sema3A对成牙骨质细胞的增殖和ALP活性无明显影响。Sema3A抑制OCN和Runx2基因的表达(P<0.05),并抑制矿化结节的形成。结论:Sema3A对成牙骨质细胞的增殖无明显影响,但对细胞的矿化功能起到抑制作用。

关键词: 信号素3A, 成牙骨质细胞, 增殖, 矿化

Abstract: Objective: To investigate the effects of Semaphorin3A (Sema3A) on the proliferation and mineralization of cementoblasts. Methods: The immortalized murine cementoblast cell line OCCM-30 was treated with recombinant human Sema3A (0, 0.5, 1mg/L). Cell proliferation was tested by MTT assay. Alkaline phosphotase (ALP) activity was detected using an ALP kit. The mRNA levels of osteocalcin (OCN), osteopontin (OPN) and Runx2 were investigated by quantitative reverse transcription PCR (RT-PCR). Calcium deposition was assayed with Alizarin red staining (ARS). Results: The proliferation and the ALP activity of OCCM-30 treated with Sema3A were not significantly different from those of the control. The OCN and Runx2 mRNA expressions of the OCCM-30 treated with Sema3A significantly declined in contrast to that of the control. Sema3A also inhibited the mineral nodule formation in vitro compared to untreated cells. Conclusion: Sema3A did not obviously affect the proliferation, but suppressed the mineralization of the cementoblasts.

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