Exendin4-PEGDA/HA矿化水凝胶的制备及体外成骨作用的研究

doi:10.13701/j.cnki.kqyxyj.2020.08.007

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (8): 731-735.DOI: 10.13701/j.cnki.kqyxyj.2020.08.007

Exendin4-PEGDA/HA矿化水凝胶的制备及体外成骨作用的研究

王岩松, 刘建林^*, 陈晓霞 ,白姝婷

广州中医药大学深圳医院广东深圳 518034

收稿日期:2019-11-25 出版日期:2020-08-28 发布日期:2020-08-18
通讯作者: *刘建林,E-mail: wangyansong15985@163.com
作者简介:王岩松(1982~ ),男,辽宁沈阳人,硕士,主治医师,主要从事种植修复临床治疗工作。
基金资助:
福田区卫生公益性科研项目(编号:FTWS2017001)

Preparation and in vitro Osteogenesis of Exendin4-PEGDA/HA Mineralized Hydrogel

WANG Yansong, LIU Jianlin^*, CHEN Xiaoxia, BAI Shuting

Shenzhen Hospital of Guangzhou University of Traditional Chinese Medicine, Shenzhen 518034, China

Received:2019-11-25 Online:2020-08-28 Published:2020-08-18

摘要/Abstract

摘要： 目的: 制备一种负载具有诱导成骨分化作用Exendin4的高强度PEGDA/HA矿化水凝胶。方法: 通过光聚合反应制备PEGDA水凝胶,用原位沉淀法制备PEGDA/HA矿化水凝胶,通过凝胶吸附作用将Exendin4载入。探讨不同钙磷浓度对PEGDA/HA水凝胶强度影响,检测Exendin4的载药量,体外释放及Exendin4-PEGDA/HA体外生物相容性和成骨活性。结果: PEGDA/HA矿化支架具有疏松多孔结构,较高的力学强度;在20 d内展现出良好缓释曲线;细胞实验显示该高强度水凝胶具有良好的生物相容性和较高的成骨活性。结论: 高强度Exendin4-PEGDA/HA水凝胶在骨样组织再生过程中展现良好的成骨活性,有作为骨组织修复生物材料的潜力。

关键词: Exendin4, PEGDA, 矿化水凝胶, 缓释

Abstract: Objective: To prepare a high strength PEGDA/HA mineralized hydrogel loaded with Exendin4 to induce the differentiation of osteoblast. Methods: PEGDA hydrogel was prepared by photopolymerization, and PEGDA/HA mineralized hydrogel was prepared by in-situ precipitation method, and Exendin4 was loaded by gel adsorption. The effects of different ratios of calcium and phosphorus concentrations on the strength of PEGDA/HA hydrogel were detected. The drug loading, Exendin4 release profile, biocompatibility and osteogenic activity of Exendin4-PEGDA/HA were also detected. Results: The PEGDA/HA mineralized scaffold had loose porous structure and high mechanical strength, meanwhile showed good sustained release curve within 20 days. Cell experiments showed that the high strength hydrogel had good biocompatibility and high osteogenic activity. Conclusion: The high strength Exendin4-PEGDA/HA showed good osteogenic activity in bone tissue regeneration, and has potential as a biomaterial for bone tissue repair.

Key words: Exendin4, PEGDA, mineralized hydrogel, sustained release

王岩松, 刘建林, 陈晓霞 ,白姝婷. Exendin4-PEGDA/HA矿化水凝胶的制备及体外成骨作用的研究[J]. 口腔医学研究, 2020, 36(8): 731-735.

WANG Yansong, LIU Jianlin, CHEN Xiaoxia, BAI Shuting. Preparation and in vitro Osteogenesis of Exendin4-PEGDA/HA Mineralized Hydrogel[J]. Journal of Oral Science Research, 2020, 36(8): 731-735.

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Exendin4-PEGDA/HA矿化水凝胶的制备及体外成骨作用的研究

Preparation and in vitro Osteogenesis of Exendin4-PEGDA/HA Mineralized Hydrogel

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