过表达突变型SH3BP2基因的Raw 264.7细胞中纤维增殖相关基因的检测

doi:10.13701/j.cnki.kqyxyj.2016.06.005

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (6): 570-574.DOI: 10.13701/j.cnki.kqyxyj.2016.06.005

过表达突变型SH3BP2基因的Raw 264.7细胞中纤维增殖相关基因的检测

占发龙^1,2 ,许舒宇³ ,王海丞³ ,李翠英^1* ,江久汇¹ ,徐欣²

1. 北京大学口腔医院中心实验室北京 100081;
2. 山东大学口腔医学院山东济南 250000;
3. 同济大学口腔医学院上海 200000

收稿日期:2016-03-09 出版日期:2016-06-26 发布日期:2016-06-22
通讯作者: 李翠英,电话:010-82195769
作者简介:占发龙(1989~ ),安徽人,男,硕士,主要从事口腔种植学临床及基础的研究工作。
基金资助:
北京市自然科学基金(编号:7092037)

Genes Related to Fibroblast Proliferation in Raw 264.7 Cells with Overexpressed Mutant SH3BP2.

ZHAN Fa-long^1,2, XU Shu-yu³, WANG Hai-cheng³, LI Cui-ying^1*, JIANG Jiu-hui¹, XU Xin².

1. Central Laboratory, School of Stomatology, Peking University, Beijing 100081, China;
2. School of Stomatology, Shandong University. Jinan 250000, China;
3. School of Stomatology, Tongji University, Shanghai 200000, China

Received:2016-03-09 Online:2016-06-26 Published:2016-06-22

摘要/Abstract

摘要： 目的:检测过表达野生型和突变型SH3BP2(p.Asp419Gly)基因的Raw264.7细胞株中表达差异基因,以明确与巨颌症纤维增殖相关的影响因素。方法:提取两种细胞株总RNA,进行基因芯片分析、数据库检索、RealTimePCR检测,最终确定纤维增殖相关基因。结果:找到表达差异基因共734个,上调基因313个,下调基因421个;综合基因芯片、数据库检索和RealtimePCR结果,发现4个与纤维异常增殖相关,分别为:CXCL10,IL10,Tnfrsf13b,Pf4。结论:发现4个纤维异常增殖相关基因,为进一步巨颌症纤维异常增殖的影响因素和机制研究奠定了基础。

关键词: 巨颌症, 纤维增殖, SH3BP2, 基因芯片

Abstract: Objective: To find genes related to fibroblast proliferation by comparing gene expression profile in RAW 264.7 cells with overexpressed wild type and mutant SH3BP2 gene. Methods: Total RNA in RAW 264.7 cells with overexpressed wild type or mutant SH3BP2 gene were extracted respectively by Trizol and analyzed by microarray to compare differences in gene expression profiles. Database search and real time PCR were performed to verify the most significant related genes in fibroblast proliferation. Results: Microarray data indicated that 734 genes show significant expression differences between two cell lines, among which, 313 genes were up-regulated and 421 down-regulated in cells with mutant SH3BP2 compared with wild type. Following investigation with database search and real time PCR, 4 genes were confirmed the top relevant genes to fibroblast proliferation: CXCL10, IL10, Tnfrsf13b and Pf4. Conclusion: Our study showed expression profiles differences between two cell lines. Four genes were verified to be closely related to fibroblast proliferation and might be the potential key component in the mechanism of Cherubism. This study provides us a bright new window into further study of the disease.

中图分类号:

R782.2

占发龙 ,许舒宇 ,王海丞 ,李翠英 ,江久汇 ,徐欣. 过表达突变型SH3BP2基因的Raw 264.7细胞中纤维增殖相关基因的检测[J]. 口腔医学研究, 2016, 32(6): 570-574.

ZHAN Fa-long, XU Shu-yu, WANG Hai-cheng, LI Cui-ying, JIANG Jiu-hui, XU Xin.. Genes Related to Fibroblast Proliferation in Raw 264.7 Cells with Overexpressed Mutant SH3BP2.[J]. Journal of Oral Science Research, 2016, 32(6): 570-574.

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过表达突变型SH3BP2基因的Raw 264.7细胞中纤维增殖相关基因的检测

Genes Related to Fibroblast Proliferation in Raw 264.7 Cells with Overexpressed Mutant SH3BP2.

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