小鼠涎腺发育晚期miRNA基因筛选及功能预测

doi:10.13701/j.cnki.kqyxyj.2017.08.013

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (8): 852-855.DOI: 10.13701/j.cnki.kqyxyj.2017.08.013

小鼠涎腺发育晚期miRNA基因筛选及功能预测

崔丽娟, 刘国勤^*

东方肝胆外科医院上海 201805

收稿日期:2017-03-07 出版日期:2017-08-20 发布日期:2017-08-28
通讯作者: 刘国勤,电话:13585594887
作者简介:崔丽娟(1982~ ),女,硕士,住院医师,主要从事涎腺组织工程研究工作。

Screening of Key MiRNAs during Salivary Gland Development in Mice and Functional Prediction.

CUI Li-juan, LIU Guo-qin^*.

Eastern Hepatobiliary Surgery Hospital, Shanghai 201805, China.

Received:2017-03-07 Online:2017-08-20 Published:2017-08-28

摘要/Abstract

摘要： 目的:筛选小鼠涎腺发育晚期差异表达的miRNA,为涎腺组织工程垫定实验基础。方法: 应用生物基因芯片构建小鼠涎腺发育胚胎第18天(E18.5)、19天(E19.5)、出生后0 d(P0)、出生后3 d(P3),4个时期下颌下腺miRNA表达谱,应用GENESPRING10.0进行Quantile normalization。基于Gene Ontology数据库,预测基因的功能。结果:建立小鼠涎腺发育晚期的miRNA表达谱。其中显著变化的有:mmu-miR-133a*,mmu-miR-721,mmu-miR-1,mmu-miR-133b,mmu-miR-133a ,mmu-miR-206。结论:显著变化的miRNA在涎腺发育过程中有可能起重要作用。

关键词: miRNA, 涎腺, 基因芯片

Abstract: Objective: To screen differential expression of miRNAs during salivary gland development in mice. Methods: Using microarrays, miRNA expression profiles were established at selected time points during development (E18.5, E19.5, P0, P3) of the murine submandibular gland. Furthermore, using GENESPRING10.0, Quantile normalization was established. According to Gene Ontology, the functions were predicted. Results: The expressive profiles of miRNA were established. The miRNAs whose expressive signal strength changed greatly were mmu-miR-133a, mmu-miR-721, mmu-miR-1, mmu-miR-133b, mmu-miR-133a, and mmu-miR-206. Conclusion: The miRNAs whose expressive signal strength changed greatly may play an important role in the salivary gland development.

Key words: MicroRNA , Submandibular gland , Microarray

中图分类号:

R782.7

崔丽娟, 刘国勤. 小鼠涎腺发育晚期miRNA基因筛选及功能预测[J]. 口腔医学研究, 2017, 33(8): 852-855.

CUI Li-juan, LIU Guo-qin.. Screening of Key MiRNAs during Salivary Gland Development in Mice and Functional Prediction.[J]. Journal of Oral Science Research, 2017, 33(8): 852-855.

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小鼠涎腺发育晚期miRNA基因筛选及功能预测

Screening of Key MiRNAs during Salivary Gland Development in Mice and Functional Prediction.

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