口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (12): 1240-1243.DOI: 10.13701/j.cnki.kqyxyj.2016.12.004

• 基础研究论著 • 上一篇    下一篇

EMP1基因对口腔鳞癌细胞增殖、迁移和侵袭影响的体外研究

张军1*,代晓华2,邹慧儒2,连小丽2,李燕妮2   

  1. 1. 天津市口腔医院口腔颌面外科 天津 300041;
    2. 天津市口腔医院中心实验室 天津 300041
  • 收稿日期:2016-09-15 出版日期:2016-12-25 发布日期:2016-12-26
  • 通讯作者: 张军,电话:022-59080648
  • 作者简介:张军(1975~ ),男,河北唐山人,博士,副主任医师,主要从事口腔颌面外科临床治疗工作。
  • 基金资助:
    天津市卫计委科技基金项目(编号:2013KZ053)

Effects of EMP1 on the Proliferation, Migration and Invasion of Oral Squamous Cancer Cells in Vitro.

ZHANG Jun1*, DAI Xiao-hua2, ZOU Hui-ru2, LIAN Xiao-li2, LI Yan-ni2.   

  1. 1. Department of Oral and Maxillofacial Surgery, Tianjin Hospital of Stomatology, Tianjin 300041, China;
    2. Research Center, Tianjin Hospital of Stomatology, Tianjin 300041, China
  • Received:2016-09-15 Online:2016-12-25 Published:2016-12-26

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摘要： 目的:体外观察EMP1基因对口腔鳞癌细胞增殖、迁移和侵袭的影响。方法:构建pEGFP-N1-EMP1表达载体,稳定转染口腔鳞癌细胞系Tb3.1,并以野生型Tb3.1和pEGFP-N1稳转的Tb3.1为对照,用MTT检测细胞增殖,绘制细胞生长曲线;流式细胞仪检测细胞周期变化;用Transwell小室检测细胞的迁移和侵袭能力;组间比较采用单因素方差分析。结果:相比野生型Tb3.1和Tb3.1-pEGFP-N1细胞,Tb3.1-pEGFP-N1-EMP1细胞生长受到抑制,同时其S+G2-M期细胞比例减少(P＜0.05),迁移和侵袭细胞数目减少(P＜0.05)。结论:EMP1在口腔鳞癌的发生、发展中作为抑癌基因可能对口腔鳞癌细胞的增殖、迁移和侵袭起抑制作用。

关键词: EMP1基因, 口腔鳞癌细胞, 转染, 增殖, 迁移, 侵袭

Abstract: Objective: To investigate the effects of EMP1 on the proliferation and invasion of oral squamous cancer cells(OSCC) in vitro. Methods: pEGFP-N1-EMP1expression vectors were constructed and transfected stably into Tb3.1 cell. Tb3.1-pEGFP-N1-EMP1 cell, wild type Tb3.1 cell and Tb3.1-pEGFP-N1 cell were tested using MTT to assay cell proliferation. Cell cycle and invasion were analyzed by flow cytometry and invasion assay, respectively. One-way ANOVA was adopted to compare among groups. Results: Compared with wild type Tb3.1 cell and Tb3.1-pEGFP-N1 cell, the growth of Tb3.1-pEGFP-N1-EMP1 cell was inhibited, and the proportion of cells in S and G2-M phase and the number of invasive cells were reduced in Tb3.1-pEGFP-N1-EMP1 cell (P＜0.05). Conclusion: EMP1 may negatively regulate the proliferation, migration and invasion of tumor cells in the oncogenesis and development of OSCC.

Key words: EMP1 Oral, squamous cancer cells, Transfection , Proliferation Migration , Invasion

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