口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (6): 597-600.DOI: 10.13701/j.cnki.kqyxyj.2017.06.005

• 基础研究论著 • 上一篇    下一篇

Satb2过表达对牙髓干细胞增殖和迁移能力的影响

杨旭, 何丽娜, 潘爽, 李艳萍, 牛玉梅*   

  1. 哈尔滨医科大学口腔医学院牙体牙髓病科 黑龙江 哈尔滨 1540000)
  • 收稿日期:2016-11-20 出版日期:2017-06-20 发布日期:2017-06-26
  • 通讯作者: 牛玉梅,E-mail:niuym@hrbmu.edu.cn
  • 作者简介:杨旭(1990~ )男,黑龙江人,医师,硕士在读,主要从事牙体牙髓疾病的临床治疗工作。
  • 基金资助:
    黑龙江省自然科学基金项目(编号:H201440)

Effect of Satb2 Overexpression on Proliferation and Migration of Human Dental Pulp Stem Cells.

YANG Xu, HE Li-na, PAN Shuang, LI Yang-ping, NIU Yu-mei*.   

  1. Department of Endodontics, Stomatology College of Harbin Medical University. Harbin 150001, China。
  • Received:2016-11-20 Online:2017-06-20 Published:2017-06-26

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摘要： 目的:通过慢病毒介导Satb2(special AT-rich binding protein 2,Satb2)感染人牙髓干细胞(human Dental pulp stem cells,hDPSCs),观察Satb2过表达对人牙髓干细胞迁移和增殖能力的影响。方法:构建Satb2过表达慢病毒感染人牙髓干细胞,通过筛选得到稳定过表达Satb2细胞克隆。CCK8实验检测过表达Satb2对人牙髓干细胞的增殖能力的影响。划痕实验和Transwell细胞迁移实验观察细胞迁移能力的变化。免疫荧光染色观察细胞骨架微管的变化。结果:过表达Satb2的人牙髓干细胞相比对照组增殖能力增强(P＜0.05),微管更粗大,细胞的迁移能力增强。结论:过表达Satb2能使人牙髓干细胞的增殖和迁移能力增强。

关键词: 特异AT序列结合蛋白2, 人牙髓干细胞, 增殖, 迁移

Abstract: Objective: To study the influence of special AT-rich binding protein 2 (Satb2) overexpressionon the proliferation and migration of human dental pulp stem cells (hDPSCs). Methods: Lentiviral-mediated gene was used to transfer Satb2 overexpression into hDPSCs, while control groups were normal hDPSCs and hDPSCs infected with empty vector. Cellular proliferation was determined by CCK8 assay. Immunofluorescence staining was performed to observe the structure of microtubules. Scratch-wound assay and migration assay were used to detect the migration of Satb2-overexpressing hDPSCs. Results: After 1, 3, 5, 7 days of cultivation, the optical density (OD) in the Satb2-overexpressing hDPSCs group was significantly higher than that in the control groups (P＜0.05). The microtubules in Satb2-overexpressing hDPSCs group were more slender than those in the control groups. Scratch-wound and migration assay showed that overexpression of Satb2 promoted the migration of hDPSCs. Conclusion: Overexpression of Satb2 promoted the proliferation and migration of hDPSCs.

Key words: Special , AT-rich binding protein 2, Human dental pulp stem cells , Proliferation, Migration

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