枯草芽孢杆菌TLO3的全基因组测序及与枯草芽孢杆菌168和XF-1的比较基因组学研究

doi:10.13701/j.cnki.kqyxyj.2018.06.007

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (6): 601-604.DOI: 10.13701/j.cnki.kqyxyj.2018.06.007

枯草芽孢杆菌TLO3的全基因组测序及与枯草芽孢杆菌168和XF-1的比较基因组学研究

张小凤¹, 黄慧敏¹, 易根云¹, 吴生荣¹, 周建业², 李志强^2*, 包广洁^1*

1. 兰州大学口腔医学院甘肃兰州 730000;
2. 西北民族大学口腔医学院甘肃兰州 730030

收稿日期:2017-11-20 出版日期:2018-06-20 发布日期:2018-06-21
通讯作者: 李志强,E-mail:Lizhiqiang6767@163.com; 包广洁,E-mail:2645784430@qq.com
作者简介:张小凤(1991~ ),女,甘肃平凉人,硕士在读,主要从事口腔医学工作。

Whole Genome Sequence of Bacillus Subtilis TLO3 and Comparative Genomics Analysis among That Strain, B.subtilis 168 and B.subtilis XF-1

ZHANG Xiao-feng¹, HUANG Hui-min¹, YI Gen-yun¹, WU Sheng-rong¹, ZHOU Jian-ye², LI Zhi-qiang^2*, BAO Guang-jie^1*

1. School of Stomatology, Lanzhou University, Lanzhou 730000, China;
2. School of Stomatology, Northwest University for Nationalities, Lanzhou 730030, China

Received:2017-11-20 Online:2018-06-20 Published:2018-06-21

摘要/Abstract

摘要： 目的:为了解枯草芽孢杆菌 TLO3的全基因组及分子生物学功能。方法:通过三代联合二代测序技术对枯草芽孢杆菌TLO3进行全基因组测序并使用相关软件对其进行基因组组装、基因预测与功能注释;并与B.subtilis168及XF-1进行比较基因组学分析。结果:枯草芽孢杆菌TLO3全基因组由1条环状染色体和质粒组成;比较基因组研究发现TLO3在细胞复制、重组和修复等过程的基因数量明显高于XF-1和168,抗菌基因簇与XF-1相似。结论:枯草芽孢杆菌TLO3在具有抗菌性的同时,还有更强的生存能力,可能更好地适应口腔复杂环境。

关键词: 枯草芽孢杆菌, 全基因组测序, 基因家族, 比较基因组

Abstract: Objective: To analyze the whole genome and molecular biological function of the B.subtilis TLO3. Methods: The whole genome sequence of B. subtilis TLO3 was performed by a combination of the three-generation and second-generation sequencing technology, and using relevant software for its genome assembly, gene prediction, functional annotation and comparative genomics analysis with B. subtilis 168 and XF-1. Results: The complete genome of B. subtilis TLO3 consists of one circular chromosome and plasmid. The number of genes in cell replication, recombination and repair in strain TLO3 was significantly higher than that of XF-1 and 168, and the antimicrobial gene cluster of TLO3 was similar to XF-1. Conclusion: B.subtilisTLO3, with antimicrobial activity, has a stronger viability and may be better adapted to the complex environment of the oral.

Key words: Bacillus Subtilis, Complete genome sequence, Gene family, Comparative genome

张小凤, 黄慧敏, 易根云, 吴生荣, 周建业, 李志强, 包广洁. 枯草芽孢杆菌TLO3的全基因组测序及与枯草芽孢杆菌168和XF-1的比较基因组学研究[J]. 口腔医学研究, 2018, 34(6): 601-604.

ZHANG Xiao-feng, HUANG Hui-min, YI Gen-yun, WU Sheng-rong, ZHOU Jian-ye, LI Zhi-qiang, BAO Guang-jie. Whole Genome Sequence of Bacillus Subtilis TLO3 and Comparative Genomics Analysis among That Strain, B.subtilis 168 and B.subtilis XF-1[J]. Journal of Oral Science Research, 2018, 34(6): 601-604.

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枯草芽孢杆菌TLO3的全基因组测序及与枯草芽孢杆菌168和XF-1的比较基因组学研究

Whole Genome Sequence of Bacillus Subtilis TLO3 and Comparative Genomics Analysis among That Strain, B.subtilis 168 and B.subtilis XF-1

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