口腔医学研究 ›› 2019, Vol. 35 ›› Issue (3): 203-207.DOI: 10.13701/j.cnki.kqyxyj.2019.03.001

• 特约述评 •    下一篇

细胞外囊泡的可视化研究

陈刚1,2*, 夏厚福1   

  1. 1. 武汉大学口腔医学院口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室 湖北 武汉 430079;
    2. 武汉大学口腔医院口腔颌面外科 湖北 武汉 430079
  • 收稿日期:2019-01-08 出版日期:2019-03-28 发布日期:2019-04-01
  • 通讯作者: * 陈刚,E-mail:geraldchan@whu.edu.cn

Visualization Research on Extracellular Vesicles

CHEN Gang1,2*, XIA Hou-fu1   

  1. 1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
    2. Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
  • Received:2019-01-08 Online:2019-03-28 Published:2019-04-01

摘要: 细胞外囊泡(extracellular vesicles, EVs)可通过介导细胞间通讯参与调控多项重要的病理生理学过程。深入研究细胞外囊泡的生物学行为和功能及其与重大疾病的相关性不仅有助于揭示相关疾病的发病机制,还有望为疾病的早期诊断及预后评估提供新的液体活检标志物。然而,目前针对细胞外囊泡的研究手段,尤其是在可视化方面还存在较大局限性,这在很大程度上阻碍了对细胞外囊泡生物学行为和功能的深入研究。因此,建立稳定可靠的示踪成像技术,实现对细胞外囊泡生物学行为和功能的可视化研究是当前细胞外囊泡相关领域的前沿热点和迫切需求。本文将简要介绍细胞外囊泡可视化研究的现状,并对其未来发展方向作一展望。

关键词: 细胞外囊泡, 可视化研究, 动态示踪

Abstract: Extracellular vesicles (EVs) are involved in the regulation of many pathophysiological processes by mediating intercellular communication. In-depth study of the biological behaviors and functions of EVs and their association with the development of diseases are the main research purpose at present. It will not only help to reveal the pathogenesis of related diseases, but will also facilitate the development of novel liquid biopsy biomarkers for early diagnosis and prognosis. However, current research methods for EVs, especially in regard to visualization of EVs, are still quite limited. This has hampered the better understanding of EVs and meanwhile posed great challenges for their potential applications. Therefore, developing reliable technologies to achieve the visualization of EVs' biological behaviors and functions is currently the frontier hot spot and urgent need in the research field of EVs. This article aims to briefly introduce the present visualization research on EVs, and prospect its future directions.

Key words: Extracellular vesicles, Visualization research, Dynamic tracking