牙本质涎蛋白通过整合素β6调节Smad蛋白活性

doi:10.13701/j.cnki.kqyxyj.2019.06.007

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (6): 537-540.DOI: 10.13701/j.cnki.kqyxyj.2019.06.007

牙本质涎蛋白通过整合素β6调节Smad蛋白活性

勾晓辉,柴纪华,袁国华^*

武汉大学口腔医学院,湖北省口腔基础医学重点实验室——省部共建国家重点实验室培育基地,口腔生物医学教育部重点实验室湖北武汉 430079

收稿日期:2019-02-04 出版日期:2019-06-28 发布日期:2019-06-27
通讯作者: 袁国华,E-mail:yuanguohua@whu.edu.cn
作者简介:勾晓辉(1992~ ),女,山西运城人,硕士,主要从事儿童口腔医学相关研究及临床工作。
基金资助:
国家自然科学基金(编号:81470708)

DSP^aa183-219 Regulates Activity of Smad Protein by Interacting with Integrin β6.

GOU Xiao-hui, CHAI Ji-hua, YUAN Guo-hua^*

State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine of Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.

Received:2019-02-04 Online:2019-06-28 Published:2019-06-27

摘要/Abstract

摘要： 目的:研究牙本质涎蛋白片段DSP^aa183-219与成牙本质细胞膜上整合素β6结合后对细胞内Smad蛋白的影响。方法:DSP^aa183-219刺激后,免疫荧光和免疫印迹检测胞内Smad蛋白磷酸化和核转移情况。整合素β6抗体阻断β6受体,DSP^aa183-219刺激后检测胞内Smad蛋白磷酸化和核转移情况。结果:DSP^aa183-219刺激后,胞内磷酸化Smad1/5/8蛋白表达上调,并向核转移;整合素β6抗体阻断后,DSP^aa183-219刺激不再影响细胞内Smad1/5/8蛋白的磷酸化水平及向核转移情况。结论:DSP^aa183-219能够通过成牙本质细胞膜上整合素β6受体来提高胞浆内Smad1/5/8蛋白的磷酸化水平并促进磷酸化Smad1/5/8向核转移。

关键词: 牙本质涎蛋白, 整合素β6, Smad蛋白, 磷酸化

Abstract: Objective: To investigate the effects of DSP^aa183-219 on the intracellular activity of Smad protein through binding to integrin β6. Methods: Immunofluorescence and western blot assay were used to detect the expression levels of Smad1/5/8 and phospho-Smad1/5/8 protein in the odontoblasts after treated with DSP^aa183-219 fragments. Anti-β6 antibody was used to inhibit integrin β6, and immunofluorescence and western blot assay were used to determine the expression pattern of Smad1/5/8 protein in the odontoblasts after treated with DSP^aa183-219 fragments. Results: After stimulated by DSP^aa183-219 fragments, phosphorylated-Smad1/5/8 in the odontoblasts was up-regulated and translocated to the nucleus. After treated with integrin β6 inhibitor (anti-β6 antibody), and followed by adding DSP^aa183-219 to the medium for different time periods, phosphorylation and nuclear translocation of Smad1/5/8 in the odontoblasts induced by DSP^aa183-219 were effectively blocked. Conclusion: DSP^aa183-219 binds to integrin β6 on the cell surface and induces Smad1/5/8 phosphorylation and nuclear translocation in the odontoblasts.

Key words: Dentin sialoprotein Integrin β6, Smad protein, Phosphorylation

勾晓辉,柴纪华,袁国华. 牙本质涎蛋白通过整合素β6调节Smad蛋白活性[J]. 口腔医学研究, 2019, 35(6): 537-540.

GOU Xiao-hui, CHAI Ji-hua, YUAN Guo-hua. DSP^aa183-219 Regulates Activity of Smad Protein by Interacting with Integrin β6.[J]. Journal of Oral Science Research, 2019, 35(6): 537-540.

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牙本质涎蛋白通过整合素β6调节Smad蛋白活性

DSP^aa183-219 Regulates Activity of Smad Protein by Interacting with Integrin β6.

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牙本质涎蛋白通过整合素β6调节Smad蛋白活性

DSPaa183-219 Regulates Activity of Smad Protein by Interacting with Integrin β6.

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DSP^aa183-219 Regulates Activity of Smad Protein by Interacting with Integrin β6.