载柚皮苷胶原蛋白凝胶对人牙髓干细胞增殖的影响

doi:10.13701/j.cnki.kqyxyj.2019.06.008

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (6): 541-545.DOI: 10.13701/j.cnki.kqyxyj.2019.06.008

载柚皮苷胶原蛋白凝胶对人牙髓干细胞增殖的影响

肖婉鲁,潘爽,侯婷婷,李艳萍,何丽娜,牛玉梅^*

哈尔滨医科大学附属口腔医院牙体牙髓病科黑龙江哈尔滨 150001

收稿日期:2019-02-11 出版日期:2019-06-28 发布日期:2019-06-27
通讯作者: 牛玉梅,E-mail:niuym@hrbmu.edu.cn
作者简介:肖婉鲁(1991~ ),女,山东人,硕士,住院医师,主要研究牙髓干细胞再生的相关研究。
基金资助:
国家自然科学基金(编号:81570963)

Effects of Collagen Gel Encapsulating Naringin on Proliferation of Human Dental Pulp Stem Cells.

XIAO Wan-lu, PAN Shuang, HOU Ting-ting, LI Yan-ping, HE Li-na, NIU Yu-mei^*

Department of Endodontics, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.

Received:2019-02-11 Online:2019-06-28 Published:2019-06-27

摘要/Abstract

摘要： 目的:构建柚皮苷/胶原蛋白凝胶复合体,研究其对人牙髓干细胞(human dental pulp stem cells,hDPSCs)体外增殖的影响。方法:体外分离并培养hDPSCs,CCK-8实验检测不同浓度的胶原蛋白凝胶对hDPSCs增殖的影响,进而筛选出最佳浓度,并以最佳浓度(1 g/L)胶原蛋白凝胶载不同质量的柚皮苷。通过CCK-8实验检测载柚皮苷胶原蛋白凝胶对hDPSCs增殖的影响。结果:1 g/L浓度的胶原蛋白凝胶更适合hDPSCs的生长;当柚皮苷与胶原蛋白凝胶质量之比为0.2∶1时能更好地促进hDPSCs的粘附和生长。结论:一定比例的柚皮苷胶原蛋白凝胶与hDPSCs具有良好的生物相容性,可作为一种理想支架材料为hDPCs的生长提供空间。

关键词: 人牙髓干细胞, 柚皮苷, 胶原蛋白凝胶, 细胞增殖

Abstract: Objective: Naringin/collagen gel complex was constructed and its effects on the proliferation of human dental pulp stem cells (hDPSCs) were investigated. Methods: HDPSCs were isolated and cultured in vitro. Proliferation ability of hDPSCs in various concentrations of collagen gel was analyzed using a cell counting kit -8 (CCK-8) assays. HDPSCs and different concentrations of naringin were encapsulated in the collagen gel. CCK-8 assay was used to evaluate the effect of naringin/collagen gel complex on the proliferation of hDPSCs. Results: The optimal collagen gel concentration was 1 g/L, which could enhance the adherence and growth of hDPSCs. In addition, the best ratio of naringin to collagen gel was 0.2∶1, which promoted the growth and proliferation of hDPSCs. Conclision: Naringin/collagen gel complex possesses a good cellular compatibility with hDPSCs. As a scaffold material, it can provide space for hDPSCs to proliferate.

Key words: Human dental pulp stem cells, Naringin, Collagen gel, Cell proliferation

肖婉鲁,潘爽,侯婷婷,李艳萍,何丽娜,牛玉梅. 载柚皮苷胶原蛋白凝胶对人牙髓干细胞增殖的影响[J]. 口腔医学研究, 2019, 35(6): 541-545.

XIAO Wan-lu, PAN Shuang, HOU Ting-ting, LI Yan-ping, HE Li-na, NIU Yu-mei. Effects of Collagen Gel Encapsulating Naringin on Proliferation of Human Dental Pulp Stem Cells.[J]. Journal of Oral Science Research, 2019, 35(6): 541-545.

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载柚皮苷胶原蛋白凝胶对人牙髓干细胞增殖的影响

Effects of Collagen Gel Encapsulating Naringin on Proliferation of Human Dental Pulp Stem Cells.

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