人舌鳞状细胞癌细胞系WU-TSC-1的建立及鉴定

doi:10.13701/j.cnki.kqyxyj.2022.04.013

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (4): 357-361.DOI: 10.13701/j.cnki.kqyxyj.2022.04.013

人舌鳞状细胞癌细胞系WU-TSC-1的建立及鉴定

郑小风^1,2, 孙雅楠^1,2, 胡雅英^1,2, 吕宜楠^1,2, 杨可^1,2, 李易玮^1,2, 张佳莉^1,2*

1.武汉大学口腔医院口腔病理科湖北武汉 430079;
2.口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室,武汉大学口腔医学院湖北武汉 430079

收稿日期:2021-08-26 出版日期:2022-04-28 发布日期:2022-04-22
通讯作者: *张佳莉,E-mail: jiali_zhang@whu.edu.cn
作者简介:郑小风(1996~ ),女,湖北宜昌人,硕士在读,研究方向:口腔组织病理学。
基金资助:
国家自然科学基金(编号:81972552)

Establishment and Identification of Human Tongue Squamous Cell Carcinoma Cell Line WU-TSC-1

ZHENG Xiaofeng^1,2, SUN Yanan^1,2, HU Yaying^1,2, LV Yinan^1,2, YANG Ke^1,2, LI Yiwei^1,2, ZHANG Jiali^1,2*

1. Department of Oral Histopathology, Hospital of Stomatology of Wuhan University, Wuhan 430079, China;
2. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China

Received:2021-08-26 Online:2022-04-28 Published:2022-04-22

摘要/Abstract

摘要： 目的: 建立1株人舌鳞状细胞癌细胞系WU-TSC-1并鉴定其基本生物学特性。方法: 将所获得的舌鳞状细胞癌患者手术切除的组织块标本进行原代培养,传代后建立舌鳞状细胞癌细胞系WU-TSC-1,显微镜下观察并记录其细胞形态和生长特性,行基因组DNA短串联重复序列分析,并对其倍增时间、凝集反应、细胞表面标记物、染色体核型和裸鼠成瘤能力等特性进行检测。结果: WU-TSC-1可稳定传代50余代,为人源肿瘤细胞,无其他细胞交叉污染。细胞呈卵圆形或方圆形,失去接触抑制。染色体核型为非二倍体染色体。体外细胞倍增时间为51.15 h,体内具有成瘤能力。结论: 该研究成功建立了1株人舌鳞状细胞癌细胞系WU-TSC-1,为揭示舌鳞状细胞癌的发病机制,探索诊断指标和治疗方案提供了新的实验细胞系。

关键词: 舌鳞状细胞癌, 细胞系, 原代培养

Abstract: Objective: To establish a human tongue squamous cell carcinoma (TSCC) cell line WU-TSC-1 and to identify its basic biological characteristics. Methods: The excised tissue from a patient with TSCC was primary cultured. After passage, the TSCC cell line WU-TSC-1 was established. Its morphology and growth characteristics were observed and recorded through microscope. Short tandem repeat analysis (STR) of genomic DNA on this cell was conducted. Its doubling time, agglutination reaction, cell surface markers, chromosome karyotype, and tumorigenic ability in nude mice were also examined. Results: WU-TSC-1 cells had been subcultured for more than 50 passages which were confirmed to be human-derived tumor cells with no other cell contamination. In vitro, the cells lost contact inhibition and had a uniform oval or squares shape and non-diploid chromosomes. Its doubling time was calculated as 51.15 h. In vivo, the cell line showed tumorigenesis ability. Conclusion: This study has successfully established a human TSCC cell line WU-TSC-1 which provided a new cell line for revealing the pathogenesis of TSCC as well as exploring useful diagnostic indicators and treatment options.

Key words: tongue squamous cell carcinoma, cell line, primary culture

郑小风, 孙雅楠, 胡雅英, 吕宜楠, 杨可, 李易玮, 张佳莉. 人舌鳞状细胞癌细胞系WU-TSC-1的建立及鉴定[J]. 口腔医学研究, 2022, 38(4): 357-361.

ZHENG Xiaofeng, SUN Yanan, HU Yaying, LV Yinan, YANG Ke, LI Yiwei, ZHANG Jiali. Establishment and Identification of Human Tongue Squamous Cell Carcinoma Cell Line WU-TSC-1[J]. Journal of Oral Science Research, 2022, 38(4): 357-361.

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人舌鳞状细胞癌细胞系WU-TSC-1的建立及鉴定

Establishment and Identification of Human Tongue Squamous Cell Carcinoma Cell Line WU-TSC-1

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